Detection of methicillin resistance in Staphylococcus species from clinical samples

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Research Paper 30/01/2022
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Detection of methicillin resistance in Staphylococcus species from clinical samples

S. Jayashree, Dr. Mita D. Wadekar, Pooja C., Dr. Sathish JV.
Int. J. Micro. Myco.14( 1), 1-6, January 2022.
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Staphylococcus aureus that are resistant to many antibiotics are known as as Methicillin-resistant Staphylococcus aureus (MRSA). Infections caused by MRSA can cause severe infection especially in immunocompromised individuals. Compared to ordinary Staphylococcus infections, MRSA infectios are more difficult to treat. This is because the strains of staphylococcus known as MRSA do not respond well to many common antibiotics. The objective of the study is to detect MRSA in Staphylococcus species isolated from various clinical samples. A retrospective study of all staphylococcus species was conducted. Total of 161 isolates were isolated from various samples. They were processed and identified by standard Microbiological procedures. The antibiotics susceptibility testing was performed by Kirby- Bauer disc diffusion method using CLSI guidelines. MRSA was detected by disc diffusion test using Cefoxitin (30μg) disc. Of 161 samples processed, 74 Methicillin-resistant Staphylococcus species were isolated. Methicillin resistance were found to be high in pus (41.8%) samples followed by urine 29(39.1%) and blood 14 (18.9%) and they showed higher sensitivity towards vancomycin followed by linezolid. Methicillin resistance in Staphylococcus species are increasing rapidly and becoming a major problem. Disk diffusion method can be routinely employed to detect these resistant strains to understand the drug resistance patterns of Methicillin resistance producing staphylococcus species.


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