Welcome to International Network for Natural Sciences | INNSpub

Detection of multi-drug resistant Streptococcus pyogenes from raw milk samples in Faisalabad

Research Paper | December 1, 2020

| Download 12

Muhammad Fahad Chaudhary, Ali Abbas Dilawar, Muhammad Asim, Suneel Ahmad, Unsa Saleem

Key Words:

Int. J. Biosci.17( 6), 353-359, December 2020

DOI: http://dx.doi.org/10.12692/ijb/17.6.353-359


IJB 2020 [Generate Certificate]


Milk is a very important source of nutrition for humans as well as animals. It contains carbohydrates, fats, vitamins, and minerals. Due to its nutritious composition, it is an excellent medium for the growth of microorganisms. Milk gets contaminated by different microorganisms including Streptococcus species.  Considering its importance, the present study was designed to check the microbial contamination of raw milk. 90 samples were collected from different areas of Faisalabad city. Mainly Streptococcus species were targeted and isolated. Out of 90, only 23 samples were found positive for Streptococcus species. Most species were identified as Streptococcus pyogenes by biochemical characteristics. Antibiotic susceptibility testing was performed and isolates were found to be susceptible to Amikacin (30µg), Tazobactam (110µg), Levofloxacin (5µg), Tobramycin (30µg), Ampicilin-sulbactam (20µg), Chloromphenicol (30µg), and Gentamycin (30µg) and were resistant to Cefoxitin (30µg), Tigecycline (15µg), Cefipime (30µ), Ampicilin (10µ), Cefotoxime (10µg), Trimethoprim (25µg), Cefuroxime (30µg), Cefixime (5µg), Tetracycline (10µg). For further confirmation, Minimum Inhibitory Concentration was performed according to CLSI guidelines and results showed that isolates were susceptible to only Ampicillin, Amikacin and Erythromycin. Animals suffering from clinical or sub-clinical mastitis are the source of Streptococcal contamination of milk. The current study supports the finding that raw milk can be regarded as a critical source of MDR bacteria. Strict monitoring and the implementation of effective hygienic should be implemented.


Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Detection of multi-drug resistant Streptococcus pyogenes from raw milk samples in Faisalabad

Elmoslemany AM, Keefe JP, Dohoo IR, Dingwell RT. 2009. Microbiological quality of bulk tank raw milk in Prince Edward Island dairy herds. Journal of Dairy Science 92(9), 4239-4248. http://dx.doi.org/10.3168/jds.2008-1751

Aijaz Hussain Smmoro, Arain MA, Khaskheli M, BachalBhutto. 2002. Isolation of Escherichia Coli from Raw Milk and Milk Products in Relation to Public Health Sold under Market Conditions at Tandojam, Pakistan. Pakistan Journal of Nutrition 1(3), 151-152. http://dx.doi.org/10.3923/pjn.2002.151.152

Carla Maria Lopes de Azevedo, Diana Pacheco, Luisa Soares, Ricardo Romao, Monica Moitoso, Jaime Maldonado, Roger Guix, Joao Simoes. 2015. Prevalence of contagious and environmental mastitis-causing bacteria in bulk tank milk and its relationships with milking practices of dairy cattle herds in São Miguel Island (Azores). Tropical Animal Health and Production 48(2), 451-459. http://dx.doi.org/10.1007/s11250-015-0973-6

Cinthia Alves-Barroco, Catarina Rodrigues, Luis Raposo R, Catarina Bras, Mario Diniz, Joao Caco, Pedro Costa M, lida Santos –Sanches, Alexandra Fernandes R. 2018. Streptococcus dysgalactiae subsp. Dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models, microbiologyOpen 8(17). http://dx.doi.org/10.1002/mbo3.623

Clinton Mcdaniel J, Diana Cardwell M, Robert Moeller B, Gregory Gray C. 2014. Humans and Cattle: A Review of Bovine Zoonoses. Vector borne and zoonotic diseases 14(1), 1-19. http://dx.doi.org/10.1089/vbz.2012.1164

Guerin-Faublee V, Tardy F, Bouveron C, Carret G. 2002. Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows. International journal of antimicrobial agents 19(3), 219-226. http://dx.doi.org/10.1016/s0924-8579(01)00485-x

Guetouache Mourad, Guessas Bettache, Medjekal Samir. 2014. Composition and nutritional value of raw milk. Issues in Biological Sciences and Pharmaceutical Research 2(10), 155-122. http://dx.doi.org/10.15739/IBSPR.005

Thaker HC, Brahmbhatt MN, Nayak JB. 2013. Isolation and identification of Staphylococcus aureus from milk and milk products and their drug resistance patterns in Anand, Gujarat. Veterinary World 5(12), 10-13, http://dx.doi.org/10.5455/vetworld.2013.10-13

Humberto Guanche Garcell E, Guilarte Garcia Pedro Vazquez Pueyo I, Rodriguez Martin, Ariadna Villanueva Arias, Ramon Alfonso Serrano N. 2016. Outbreaks of brucellosis related to the consumption of unpasteurized camel milk. Journal of Infection and Public Health 9(4). http://dx.doi.org/10.1016/j.jiph.2015.12.006

Jan Hudzicki. 2009. kirby-bauer disk diffusion susceptibility test protocol, American Society For Microbiology (ASM)(December 2009), 1–13, https://www.asm.org/Protocols/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test-Pro

Jeykumar, Vinodkumar G, Bimal P Bashir, Sudhakar Krovvidi. 2013. Antibiogram of mastitis pathogens in the milk of crossbred cows in Namakkal district, Tamil Nadu, Veterinary World 6(6), 354-356. http://dx.doi.org/10.5455/vetworld.2013.354-356

Prejit Nanu E, Latha C. 2007. Microbial quality assurance of milk during production, processing and marketing.  American Journal of Food Technology 2(3), 136-144, http://dx.doi.org/10.3923/ajft.2007.136.144

Silpi Basak, Priyanka Singh, Monali Rajurkar. 2016. Multidrug resistant and extensively drug resistant bacteria: A Study. Journal of Pathogens 1-5, http://dx.doi.org/10.1155/2016/4065603

Stephen Oliver P, Bhushan Jayarao M, Raul Almeida A. 2005. Foodborne Pathogens in Milk and the Dairy Farm Environment: Food Safety and Public Health Implications. Foodborne pathogens and disease 2(2), 115-29, http://dx.doi.org/10.1089/fpd.2005.2.115

Wiegand I, Hilpert K, Hancock RE. 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 3(2), 163-175, http://dx.doi.org/10.1038/nprot.2007.521

Yasser Haggag N, Mohamed Nossair A, Alaa Mansour M, Amir Abd el Rahman. 2018. Streptococci in Dairy Farms: Isolation, Antibiogram Pattern and Disinfectant Sensitivity. Alexandria Journal of Veterinary Sciences  59(2),  85-92, http://dx.doi.org/10.5455/ajvs.8031