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Detection of natural cryptosporidial infection in slaughtered broiler chicken in local markets of Baghdad province

Research Paper | November 1, 2018

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Atheer Kareem Kadhim, Mohammed Thabit Salih Al-Zubaidi

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Int. J. Biosci.13( 5), 141-150, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.141-150


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The present study aimed investigate the detection of  cryptosporidiosis in 280 fecal samples from slaughtered broiler chicken carcasses in local markets in some areas of Baghdad city (AL-Aameriah, AL-Bayaa, AL-Doura Al –Kadhimiya, AL-Yarmok, AL-Turath, and Al-Shuala ), during the period from the beginning of  November 2017 to end of April 2018. Four laboratory diagnostic techniques used, flotation by Sheather’s sugar solution, staining with Modified Zeihl-Neelsen stain, measuring of isolated Cryptosporidium oocysts (by ocular micrometer) and histopathological examination for natural infected samples to determine the type of Cryptosporidium species by measuring of oocystsand site of pathological lesion, and for conform that the isolated species of parasite from infected cases belong to the C.baileyi. The study demonstrated that the overall percentage of infection was 33.21%. The highest infection rate recorded in AL-Bayaa 47.5% (19/40), while the lowest rate recorded in AL-Yarmok 22.5% (8/40) with significant deference among regions. The results showed that the highest infection rate of Cryptosporidium parasite is occured in March and April which reach to46.80 %( 22/47), 41 %( 20/48) respectively. The results of calibration of isolated oocysts, showed that the mean of measurement size of Cryptosporidium oocyst was 6.2x 4.7 micrometers. The histopathological section made to the specimens of natural infected samples ( small intestine and trachea) of slaughtered broiler chicken recoded the pathological lesions in these infected tissues. In conclusion: The total infection of Cryptosporidium in fecal samples was 33.21% (93/280).


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