Detection of natural infection and reaction of tomato lines to potato virus Y in Pakistan

Paper Details

Research Paper 01/07/2017
Views (299) Download (72)

Detection of natural infection and reaction of tomato lines to potato virus Y in Pakistan

Adnan Ahmad, Muhammad Ashfaq, Tariq Mukhtar, Saad Imran Malik, Irfan Anwer, Muhammad Ahsan, Tazeem Riaz
Int. J. Biosci.11( 1), 343-350, July 2017.
Certificate: IJB 2017 [Generate Certificate]


Tomato (Solanum lycopersicum L.) is an important solanaceous crop worldwide including Pakistan. In Pakistan the successful production of tomato is hampered due to many viral diseases including PVY which causes havoc and colossal yield losses. For management of plant viruses, accurate and proper identification of plant viruses and resistance sources is very significant. In present study, a total of 595 tomato samples with symptoms like mosaic, vein chlorosis and mild mottling were collected from tomato fields in Pakistan. All symptomatic samples were screened for the presence of Potato virus Y by DAS-ELISA using virus specific polyclonal antiserum (Bioreba AG, Switzerland). Among symptomatic samples, 104 were positive for PVY infection, of which, only eight were further screened for the presence of PVY by RT-PCR using primer pair PVYPK-F/R, that resulted in amplification of 1050 bp fragments. A total of 1050 nucleotides were obtained by sequencing each amplicon comprising a full length coat protein gene including 300 bases of UTR. The sequences of two isolates were submitted to Genbank under accession number KX816568 and KX816570. The isolate AARTPK (KX816568) was used in screening of 11 tomato cultivars. The cultivars; Kalam, NSC-92, Yaqui were found resistant (R), Rio-grandi as moderately resistant (MR) and Super-SPC and Giant-cluster as moderately susceptible (MS). Similarly, the response of BSS-30 and Gala was recorded as susceptible (S) and of Junny-2144, CKD-267 and Jagular as highly susceptible (HS). The identified resistant cultivars can be used as genetic source in developing resistant varieties against PVY in future.


Abbas MF, Hameed S, Rauf A, Nosheen Q, Ghani A, Qadir A, Zakia S. 2012. Incidence of six viruses in potato growing areas of Pakistan. Pakistan Journal of Phytopathology 24, 44-47.

Ahmad A, Ashfaq M. 2017. First report of chilli veinal mottle virus in tomato in Pakistan. Journal of Plant Pathology 99, 287-304.

Ahmad N, Khan MA, Khan NA, Binyamin R, Khan MA. 2011. Identification of resistance source in potato germplasm against PVX and PVY. Pakistan Journal of Botany 43, 2745-2749.

Alam MJ, Ashraf KUM, Gupta SD, Emon MAK. 2013. Computational approach for the prediction of potential MHC binding peptides and epitope mapping in order to develop sero-diagnostic immunogenic against potato virus Y. International Journal of Computational Bioinformatics and In Silico Modelling 2, 186-198.

Ali MC, Maokac T, Natsuakid KT, Natsuakia T. 2010. The simultaneous differentiation of Potato virus Y strains including the newly described strain PVYNTNNW by multiplex PCR assay. Journal of Virological Methods 165, 15-20.

Anith KN, Momol MT, Kloepper JW, Marois JJ, Olson SM, Jones JB. 2004. Efficacy of plant growth-promoting rhizobacteria, acibenzolar-S-methyl, and soil amendment for integrated management of bacterial wilt on tomato. Plant Disease 88, 669-673.

Aramburu J, Galipienso L, Matas M. 2006. Characterization of potato virus Y isolates from tomato crops in northeast Spain. European Journal of Plant Pathology115, 247-258.

Ashfaq M, Iqbal S, Mukhtar T, Shah H. 2014. Screening for resistance to cucumber mosaic cucumo-virus in chilli pepper. Journal of Animal and Plant Sciences 24, 791-795.

Ashfaq M, Saeed U, Mukhtar T, Haq MI. 2015. First report of Zucchini yellow mosaic virus in ridge gourd in Pakistan. Plant Disease 99, 1870.

Ashfaq M. 2007. Characterization of epidemiological and biochemical factors in relation to resistance against Urdbean Leaf Crinkle Virus (ULCV) and its management. PhD Thesis. Department of Plant Pathology Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan.

Aslam MN, Mukhtar T, Ashfaq M, Asad MJ, Hussain MA. 2015. Incidence and prevalence of bacterial wilt of chili in Punjab, Pakistan. Mycopathology 13, 37-41.

Chohan S, Perveen R, Mehmood MA, Rehman AU. 2016. Fungi colonizing different parts of tomato plant (Lycopersicon lycopersicum L.) Karst. in Pakistan. Pakistan Journal of Phytopathology 28, 25-33.

Clark MF, Adams AN. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475-483.

Danci O, Ziegler A, Torrance L, Gasemi S, Danci M. 2009. Potyviridae family-short review. The Journal of Horticultural Science and Biotechnology 3, 410-420. www.journal-hfb.usab

Dombrovsky A, Huet H, Chejanovsky H, Raccah B. 2005. Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein. Archives of Virology 150, 287-298.

FAOSTAT. 2014. Food and Agricultural Organization of the United Nations. Rome. Accessed on 15 June, 2015.

Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA. 2005. Virus Taxonomy: Classification and Nomenclature of Viruses. 8th Report of the International Committee on the Taxonomy of Viruses. New York: Elsevier Academic Press, 1259.

Georgiev KH, Vladimirov B, Baralieva D. 1988. Venera- a new tomato variety for canning. Restenievdni-Nauki 25, 77-80.

Gibbs AJ, Ohshima K, Phillips MJ, Gibbs MJ. 2008. The prehistory of potyviruses: their initial radiation was during the dawn of agriculture. PLoS One 3, 2523.

Gray S, Boer SD, Lorenzen J, Karasev A, Whitworth J, Nolte P, Singh R, Boucher A, Xu H. 2010. Potato virus Y: An evolving concern for potato crops in the United States and Canada. Plant Disease 94, 1384-1397.

Green SK, Kim JS. 1991. Characteristics and control of viruses infecting peppers: a literature review. Asian Vegetable Research and Development Centre. Technical Bulletin No. 18, 60.

Horvath J. 1983. New artificial hosts and non-hosts of plant viruses and their role in the identification and separation of viruses. XVIII. Concluding remarks. Acta Phytopathologicaet Entomologica Hungarica 18, 121-161.

Iqbal U, Mukhtar T, Iqbal SM. 2014. In vitro and in vivo evaluation of antifungal activities of some antagonistic plants against charcoal rot causing fungus, Macrophomina phaseolina. Pakistan Journal of Agricultural Sciences 51, 689-694.

Iqbal U, Mukhtar T. 2014. Morphological and pathogenic variability among Macrophomina phaseolina isolates associated with mungbean (Vigna radiata L.) Wilczek from Pakistan. The Scientific World Journal.

Kamran M, Anwar SA, Javed N, Khan SA, Haq IU, Ullah I. 2012. Field evaluation of tomato genotypes for resistance to Meloidogyne incognita. Pakistan Journal of Zoology 44, 1355-1359.

Karasev AV, Gray SM. 2013. Genetic diversity of Potato virus Y complex. American Jouranl of Potato Research 90, 7-13.

Karasev AV, Nikolaeva OV, Hu X, Sielaff Z, Whitworth J, Lorenzen JH, Gray SM. 2010. Serological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PVYN misidentification. American Journal of Potato Research, 87, 1-9.

Kayani MZ, Mukhtar T, Hussain MA. 2017. Effects of southern root knot nematode population densities and plant age on growth and yield parameters of cucumber. Crop Protection 92, 207-212.

Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N. 2010. Chilli peppers-a review on tissue culture and transgenesis. Biotechnology Advances 28, 35-48.

Lohano HD. Mari FM. 2005. Spatial Price linkages in regional onion markets of Pakistan. Journal of Agricultural and Social Sciences 1, 318-21.

Nie X, Singh M. 2013. Response of potato, tobacco and Physalis floridana plants to mixed infection with PVX, PVYNTN and PVY° strains. Canadian Journal of Plant Pathology 35, 390-401.

Revers F, García JA. 2015. Chapter Three-Molecular Biology of Potyviruses. Adv. Virus Research 92, 101-199.

Shukla DD, Ward CW, Brunt AA. 1994. The Potyviridae. CAB International, Wallingford, UK. 516.

Spetz C, Taboada AM, Darwich S, Ramsell J, Salazar LF, Valkonen JPT. 2003. Molecular resolution of a complex of potyvirus infecting solancaeous crops at the centre of origin in Peru. Journal of General Virology 84, 2565-2578.

Tahir A, Shah H, Sharif M, Akhtar W, Akmal N. 2012. An overview of tomato economy of Pakistan: Comparative analysis. Pakistan Journal of Agricultural Research 25, 4.

Tiwari JK, Mehta N, Singh MK, Tiwari PS. 2012. Screening of tomato genotypes against bacterial wilt (Ralstonia solanacearum) under field condition for Chhattisgarh. Global Journal of Biosciences and Biotechnology 1, 168-170.