Detection of Plasmid Mediated Qnr Genes in Clinical Isolates of ESBL Producing Enterobacteriaceae from Tertiary Care Hospital in Rawalpindi, Pakistan

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Research Paper 01/01/2021
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Detection of Plasmid Mediated Qnr Genes in Clinical Isolates of ESBL Producing Enterobacteriaceae from Tertiary Care Hospital in Rawalpindi, Pakistan

Muhammad Uzair Mansoor, Naureen Ehsan Ilahi, Muhammad Tamour Ashiq, Saima Ishtiaq, Muhammad Arshad Malik
Int. J. Biosci.18( 1), 53-59, January 2021.
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ESBL producing Enterobacteriacae harbouring multidrug-resistant plasmids is an emerging worldwide threat. One of the major concerns is the prevalence of plasmid-mediated quinolone resistance (PMQR) to Quinolones which has hindered the viability of these antibiotics. To date, very little data is available regarding the co-existence of ESBL and PMQR in Pakistan. This study aimed to detect PMQR genes in clinical isolates of ESBL producing Enterobacteriaceae in the city of Rawalpindi. A total of 60 clinical ESBL positive Enterobacteriaceae isolates were obtained from hospitalized patients at Holy family Hospital, Rawalpindi. It included strains of E. coli (n=24), K. pneumoniae (n=25), and Enterobacter spp (n=11). Antimicrobial susceptibility was determined by the standard disk diffusion method. Genes encoding quinolone resistance (qnrA, qnrB and qnrS) were detected using PCR. Out of 60 tested strains, 38 (63%) yielded PMQR determinants. QnrB was the most prevalent occurring alone and in combination with other qnr genes in about 89% (34/38) of the ESBL producing isolates. It was followed by qnrS (22/38 i.e 58%) and qnrA (12/38 i.e 32%). E.coli was the predominant qnrA (43%) and qnrS (69%) harbouring species.


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