Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | February 1, 2014

| Download 1

Detection of the prevalence of Aeromonas hyrophila in shrimp samples by polymerase chain reaction (PCR) and cultural method in the Iran

Faham Khamesipour, Mitra Moradi, Esmat Noshadi, Manouchehr Momeni Shahraki

Key Words:

J. Bio. Env. Sci.4(2), 47-52, February 2014


JBES 2014 [Generate Certificate]


Shrimp is one of the most important fishery products of Persian Gulf coastal provinces of Iran. Genus Aeromonas has emerged as an important human pathogen because of suspected food-borne outbreaks and the increased incidence of its isolation from patients with traveller’s diarrhea. This study was conducted to determine the prevalence rate of A. hydrophila in shrimp caught off the south coast of Iran. A total of 36 shrimp were collected in one provinces along Persian Gulf in the south coast of Iran. Samples were collected from September 2013 to May 2013. Using conventional bacteriological techniques, 14 A. hydrophila isolates were identified in which 5 strains were confirmed by PCR assay targeting 16S rDNA gene of A. hydrophila. Using PCR assays targeting the A. hydrophila 16S rDNA gene, 5 (13.888%) isolates were positive. To our knowledge, the present study is the isolation of A. hydrophila from shrimp in Iran.


Copyright © 2014
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Detection of the prevalence of Aeromonas hyrophila in shrimp samples by polymerase chain reaction (PCR) and cultural method in the Iran

Bin Kingombe CI, Huys G, Howald D, Luthi E, Swings  J,  Jemmi  T.  2004.  The  usefulness  of molecular techniques to assess the presence of Aeromonas spp.  harboring  virulence  markers  in foods.  International  Journal  of  Food  Microbiology 94, 113-121. http://dx.doi.org/10.1016/S0168-1605(03)00105-3

Chopra  AK,  Houston  CW.  1999.  Enterotoxins in Aeromonas associated gastroenteritis. Microbes and Infection 1, 1129-1137. http://dx.doi.org/10.1016/S1286-4579(99)00202-6

Chu WH, Lu CP. 2005. Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila. Journal of Fish Diseases 28, 437-441.

Colakoglu FA, Sarmasik A, Koseoglu B. 2006. Occurrence of Vibrio spp. and Aeromonas spp. in shellfish harvested off Dardanelles of Turkey. Food Control 17, 648-652.

Devlieghere F, Lefevere I, Magnin A, Debevere J. 2000. Growth of Aeromonas hydrophila in modified-atmospherepacked  cooked  meat  products. Food Microbiology 17, 185-196.

Dorsch M, Ashbolt NJ, Cox PT, Goodman AE. 1994. Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: a molecular approach based on screening of environmental isolates. Journal of Applied Bacteriology 77, 722-726.

Ebrahimzadeh Mousavi HA, Akhondzadeh Basti A, Mirzargar SS, Soltani M, Taheri Mirghaed A, Esmaeili H, Firouzbakhsh F. 2011. Vibro parahaemolyticus in shrimps and their environment in South Iran cultured. International Journal of Veterinary Research 5(3), 149-150.

Gobat PF, Jemmi T. 1993. Distribution of mesophilic Aeromonas species in raw and ready-to-eat fish and meat products in Switzerland. International Journal of Food Microbiology 20, 117-120. DOI:10.1016/0168-1605(93)90099-3.

Hudson JA, De Lacey KM. 1991. Incidence of motile aeromonads in New Zealand retail foods. Journal of Food Protection 54, 696-699.

Illanchezian S, Jayaraman S, Manoharan MS, Valsalam S. 2010. Virulance and cytotoxicity of seafood borne Aeromonas hydrophila. Brazilian Journal of Microbiology 41, 978-983. http://dx.doi.org/10.1590/S1517-838220100004000016

Khamesipour F, Khodadoustan Shahraki A, Moumeni M, Khadivi Boroujeni R, Yadegari M. 2013. Prevalence of Listeria monocytogenes in the crayfish (Astacus leptodactylus) by polymerase chain reaction in Iran. International Journal of Biosciences 3(10), 160-169. http://dx.doi.org/10.12692/ijb/3.10.160-169

Khamesipour F, Noshadi E, Moradi M. 2014 Detection of Vibrio spp. in shrimp from aquaculture sites in Iran using polymerase chain reaction (PCR). AACL Bioflux 7 (1), 1-7.

Kirov SM. 2003. Aeromonas species, 553–575. In: Hocking AD. (ed.), Foodborne Microorganisms of Public Health Significance. AIFST Inc., 752.

Niamah AK. 2012. Detected of aero gene in Aeromonas hydrophila isolates from shrimp and peeled shrimp samples in local markets. Journal of Microbiology, Biotechnology and Food Sciences 2 (2), 634-639.

Ottaviani D, Santarelli S, Bacchiocchi S, Masini L, Ghittino C, Bacchiocchi I. 2006. Occurrence and characterization of Aeromonas spp. in mussels from the Adriatic Sea. Food Microbiology 23, 418-422. http://dx.doi.org/10.1016/j.fm.2005.08.001

Palu AP, Gomes LM, Miguel MAL, Balassiano IT, Queiroz MLP, Freitas-Almeida AC, de Oliveir SS. 2006. Antimicrobial resistance in food and clinical Aeromonas isolates. Food Microbiology 23, 504-509.

Panangala VS, Shoemaker CA, van Santen VL, Dybvig K, Klesius PH. 2007. Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila. Diseases of aquatic organisms 74, 199-208. doi:10.3354/dao074199.

Parker JL, Shaw JG. 2011. Aeromonas spp. Clinical microbiology and disease. Journal of Infection 62, 109-118. http://dx.doi.org/10.1016/j.jinf.2010.12.003

Radu S, Ahmad N, Ling FH, Reezal A. 2003. Prevalence and resistance to antibiotics for Aeromonas species from retail fish in Malaysia. International Journal of Food Microbiology 81, 261-266. http://dx.doi.org/10.1016/S0168-1605(02)00228-3

Sambrook J, Russell DW. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 517.

Swaminathan TR, Rathore G, Abidi R, Kapoor D. 2004. Detection of Aeromonas hydrophila by polymerase chain reaction. Indian J. Fish 51(2), 251-254.

Tsai GJ, Chen TW. 1996. Incidence and toxigenicity of Aeromonas hydrophila in seafood. International Journal of Food Microbiology 31, 121-131.

Vivekanandhan G, Hatha AAM, Lakshmanaperumalsamy P. 2005. Prevalence of Aeromonas hydrophila in  fish  and  prawns  from the seafood market of Coimbatore, South India. Food Microbiology 22, 133-137. http://dx.doi.org/10.1016/j.fm.2004.01.015

Xanthopoulos  V,  Tzanetakis  N,  Litopoulou-Tzanetaki E. 2010. Occurrence and characterization of Aeromonas hydrophila and Yersinia enterocolitica in minimally processed fresh vegetable salads. Food Control 21, 393-398. http://dx.doi.org/10.1016/j.foodcont.2009.06.021

Yousr AH, Napis S, Rusul GRA, Son R. 2007. Detection of Aerolysin and Hemolysin Genes in Aeromonas spp. Isolated from Environmental and Shellfish Sources by Polymerase Chain Reaction. ASEAN Food Journal 14 (2), 115-122.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background