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Detection of the prevalence of Aeromonas hyrophila in shrimp samples by polymerase chain reaction (PCR) and cultural method in the Iran

Faham Khamesipour, Mitra Moradi, Esmat Noshadi, Manouchehr Momeni Shahraki

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J. Bio. Env. Sci.4(2), 47-52, February 2014

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Abstract

Shrimp is one of the most important fishery products of Persian Gulf coastal provinces of Iran. Genus Aeromonas has emerged as an important human pathogen because of suspected food-borne outbreaks and the increased incidence of its isolation from patients with traveller’s diarrhea. This study was conducted to determine the prevalence rate of A. hydrophila in shrimp caught off the south coast of Iran. A total of 36 shrimp were collected in one provinces along Persian Gulf in the south coast of Iran. Samples were collected from September 2013 to May 2013. Using conventional bacteriological techniques, 14 A. hydrophila isolates were identified in which 5 strains were confirmed by PCR assay targeting 16S rDNA gene of A. hydrophila. Using PCR assays targeting the A. hydrophila 16S rDNA gene, 5 (13.888%) isolates were positive. To our knowledge, the present study is the isolation of A. hydrophila from shrimp in Iran.

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Detection of the prevalence of Aeromonas hyrophila in shrimp samples by polymerase chain reaction (PCR) and cultural method in the Iran

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