Detection, susceptibility and molecular characterisation of ESBL- producing E. coli causing urinary tract infection

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Research Paper 01/07/2014
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Detection, susceptibility and molecular characterisation of ESBL- producing E. coli causing urinary tract infection

Fereshteh Javadian, Zahra Sepehri, Hamideh Khaje, Raziyeh Farazmand, Zahra Miri, Naghmeh Gholipoura, Zahra Shahi
J. Bio. Env. Sci.5( 1), 291-299, July 2014.
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In this study we evaluate role of a rapid polymerase chain reaction (PCR) assay compared with traditional empiric therapy in extended spectrum b-lactamases production E. coli detection, using a literature-derived model. A cross-sectional study was performed. Sample were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2010- 2011. Ninety isolates of E. coli from urinary tract infection were collected, tested for antibiotics with disc diffusion method, minimum inhibitory concentration (MIC) for ceftazidime and resistant gene TEM were detected by PCR. The result showed forty out of ninety E. coli isolates were ESBLs producing organisms by disc diffusion. Antibiotic susceptibility of E. coli isolates was evaluated for 9 antimicrobial. However, overall, E. coli were resistance to 9 of the agent including ceftriaxone (100%), ceftazidime (100%) amoxicillin (100%), azithromycin (95%), cefixime (87.5%), tetracyclin (87.5%), erythromycin (100%). nalidixin acid (85%) and difloxcain (75%) respectively. The distribution of antibiotic-resistant TEM gene according to PCR was 30%. Totally 82.5% were MIC observed as ceftazidime-resistant. We conclude that the TEM gene PCR assay is a rapid, sensitive and clinically useful test, particularly for the early detection of ESBLs- producing E. coli.


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