Detection, susceptibility and molecular characterisation of ESBL- producing E. coli causing urinary tract infection

Paper Details

Research Paper 01/07/2014
Views (254) Download (6)
current_issue_feature_image
publication_file

Detection, susceptibility and molecular characterisation of ESBL- producing E. coli causing urinary tract infection

Fereshteh Javadian, Zahra Sepehri, Hamideh Khaje, Raziyeh Farazmand, Zahra Miri, Naghmeh Gholipoura, Zahra Shahi
J. Bio. Env. Sci.5( 1), 291-299, July 2014.
Certificate: JBES 2014 [Generate Certificate]

Abstract

In this study we evaluate role of a rapid polymerase chain reaction (PCR) assay compared with traditional empiric therapy in extended spectrum b-lactamases production E. coli detection, using a literature-derived model. A cross-sectional study was performed. Sample were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2010- 2011. Ninety isolates of E. coli from urinary tract infection were collected, tested for antibiotics with disc diffusion method, minimum inhibitory concentration (MIC) for ceftazidime and resistant gene TEM were detected by PCR. The result showed forty out of ninety E. coli isolates were ESBLs producing organisms by disc diffusion. Antibiotic susceptibility of E. coli isolates was evaluated for 9 antimicrobial. However, overall, E. coli were resistance to 9 of the agent including ceftriaxone (100%), ceftazidime (100%) amoxicillin (100%), azithromycin (95%), cefixime (87.5%), tetracyclin (87.5%), erythromycin (100%). nalidixin acid (85%) and difloxcain (75%) respectively. The distribution of antibiotic-resistant TEM gene according to PCR was 30%. Totally 82.5% were MIC observed as ceftazidime-resistant. We conclude that the TEM gene PCR assay is a rapid, sensitive and clinically useful test, particularly for the early detection of ESBLs- producing E. coli.

VIEWS 14

Anilakumar KR, Sarthe V, Farhat K, Bawa AS. 2009. Ameliorative effect of ajwain extract on hexachlorocyclohexane-induced lipid peroxidation in rat liver. Food and Chemical Toxicology 47, 279-282. http://dx.doi.org/10.1016/j.fct.2008.09.061

Al-Zahrani AJ, Akhtar N. 2005. Susceptibility Patterns of Extended Spectrum ß- Lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolated in a teaching hospital. Pakistan Journal of medicinal Research 44 (2).

Anushia C, Sampathkamur P, Ramkumar L 2009. Antibacterial and antioxidant activities in Cassia auriculata. Global Journal of Pharmacology 3(3), 127-130.

Aboaba O, Efuwape BM. 2001.Antibacterial Properties of Some Nigerian Species. Biochemical and Biophysical Research Communications 13, 183-188.

Ahmed OB, Omar AO, Asghar AO, Elhassan MM. 2013.Prevalence of TEM, SHV and CTX-M genes in Escherichia coli and Klebsiella spp Urinary Isolates from Sudan with confirmed ESBL phenotype. Life Science Journal 10 (2).

Alipourfard I,Yeasmin Nili N. 2010. Antibiogram of Extended Spectrum Beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumonia isolated from Hospital Samples. Bangladesh Journal of Medical Microbiology 4 (1), 32-36. http://dx.doi.org/10.3329/bjmm.v4i1.8467

Aminzadeh Z, Sadat KM, Sha’bani M.  2008. Bacteriuria  by  extended  spectrum  Beta-lactamase-producing E. coli and Klebsiella pneumoniae: isolates in a governmental hospital in South of Tehran, Iran Iranian Journal of Kidney Diseases 2, 197-200.

Bradford P. 2001. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clinical Microbiology Reviews 14, 933- 951. http://dx.doi.org/ 10.1128/CMR.14.4.933-951.2001

Blomgran R, Zheng L, Stendahl O. 2004. Uropathogenic Escherichia coli trigger oxygen-dependent apoptosis in human neutrophils through the cooperative effect of type 1 fimbriae and lipopolysaccharide. Infection and Immunity 72, 4570-4578. http://dx.doi.org /10.1128/IAI.72.8.4570-4578.2004

Bradford PA. 2001. Extended spectrum beta-lactamases in the 21st century:characterization, epidemiology and the detection of this important resistance threat. Clinical Microbiology Reviews 14, 933-951. http://dx.doi.org /10.1128/CMR.14.4.933-951.2001

Baron EJ, Finegold SM. 1990. Bailey and Scott’s Diagnostic Microbiology, 171-178. 8th Ed. Mosby, St. Louis

Wayne Pa. 2007. Performance standards for antimicrobial disk susceptibility testing- 11th ed. Approved Standard M100-S17. Clinical and Laboratory Standards Institute. USA.

Gold HS, Moellering RC. 1996. Antimicrobial Drug Resistance. The New England Journal of Medicine 335, 1442-1454. http://dx.doi.org /10.1056/NEJM199611073351907

Fauci A, Longo D, Braunwald E. 2008. Harrison’s  principles of internal medicine. 17thed. USA: McGraw-Hill, 858.

Holt JG, Krieg NR, Sneath PH, Staley JT, Williams T, Hensyl WR.1994. Harada Y, Morinaga Y, Yamada K, Migiyama Y, Nagaoka K, Uno N, Nakamura S. 2013. Clinical and Molecular Epidemiology of Extended-Spectrum β-lactamase-Producing Klebsiella pneumonia and Escherichia Coli in a Japanese Tertiary Hospital. Journal of Medical Microbiology & Diagnosis 2(3), 1-4. http://dx.doi.org/10.4172/2161-0703.1000127

Jabeen K, Zafar A, Hasan R. 2005. Frequency and sensitivity pattern of extended-spectrum β-lactamase producing isolates in a tertiary care hospital laboratory of Pakistan. Journal of Pakistan Medical Association 55, 436-439.

Jalalpoor SH, Mobasherizadeh S. 2009. Frequence of ESBLs and antibiotic resistant pattern in to E.coli and K.pneumoniae strains isolated of hospitalized and out patients acquired urinary tract infection (Esfahan/2008-2009). Journal of Microbial World 2(2), 105-111.

Jalalpour Sh, Mobasherizadeh S. 2011. Prevalence of extended-spectrum betalactamase in Escherichia coli strains isolated from urinary tract infection. Zahedan Journal of Research in Medical Sciences 13(6), 47.

Johann DDP, Laupland KB. 2008. Extended-spectrum β-lactamase-producing Enterobacteriaceae: an emerging public-health concern. The Lancet Infectious Diseases 8, 159-166. http://dx.doi.org/10.1016/S1473-3099 (08)70041-0

Jalalpour Sh. 2011. Survey frequency of extended-spectrum betalactamases (ESBLs) in Escherichia coli and Klebsiella pneumoniae strains isolated from urinary tract infection in Iran. African Journal of Microbiology Research 5(22), 3711-3715.

Jha N, Bapats K. 2005. A study of sensitivity and resistance of pathogenic microorganisms causing UTI in Kathmandu valley. Kathmandu University Medical Journal 3, 123-129.

Khaleghi M, Kasra Kermanshahi R, Yaghoobi MM, Zarkesh-Esfahani SH., 2010. Assessment of bile salt effects on S-layer production, slp gene expression, and some physicochemical properties of Lactobacillus acidophilus ATCC4356. Journal of Microbiology and Biotechnology 20, 749-756.

Kim YK, Pai H, Lee HJ, Park SE, Choi EH, Kim J. 2002. Bloodstream infections by extended-spectrum β- lactamase-producing Escherichia coli and Klebsiella pneumoniae in children: epidemiology and clinical outcome. Antimicrobial Agents and Chemotherapy 46, 1481-1491. http://dx.doi.org/10.1128/AAC.46.5.1481-1491.2002

Kotra LP, Samama J, Mobashery S. 2002. Beta-lactamases and their resistance to beta-lactam antibiotics. In: Lewis K, Salyers AA, Taber HW, Wax RG eds. Bacterial resistance to antimicrobials. New York: Marcel Decker, 123-60.

Kalematizadeh E, 2008. Determination of extended-spectrum beta-lactamases bacteria in Escherichia coli and Klebsiella pneumoniae. Mashhad: Mashhad University of Medical Sciences.

Mansouri M, Ramazanzadeh R. 2009. Spread of extended-spectrum beta-lactamase producing Esherichia coli clinical isolates in Sanandaj hospitals. Journal of Biological Sciences 9, 362-366.

Mohammadi M, GYhasemi E, Mokhayeri H, Purina and Boroun H. 2010. Antimicrobial Resistance Patterns of E. coli Detected from Hospitalized Urine Culture Samples. Asian Journal of Biological Sciences 3, 195-201.

Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. 2010. Antimicrobial resistance among producers and non-producers of extended spectrum betalactamases in urinary isolates at a tertiary hospital in Tanzania. BMC Res Notes, 3,348.

Okeke IN, Fayinka ST, Lamikanra A, Awolowo O. 2000. Antibiotic Resistance in Escherichia coli from Nigerian Students, 1986-1998. Emerging Infectious Diseases 6(4). http://dx.doi.org/ 10.3201/eid0604.009913

Peter-Getzlaff S. 2011. Detection of AmpC beta-lactamase in Escherichia coli: comparison of three phenotypic confirmation assays and genetic analysis. Journal of Clinical Microbiology 49(8), 2924-32. http://dx.doi.org/10.1128/JCM.00091-11

Ramazanzadeh R, Farhadifar F, Mansouri M .2010. Etiology and antibiotic resistance pattern of community-acquired extended-spectrum beta-lactamas-producing gram negative isolates in Sanandaj. Research Journal of Medical Sciences 4, 243-247.

Rajabnia-Chenari M, Gooran S, Fazeli F, Dashipour A. 2012. Antibiotic resistance pattern in urinary tract infections in Imam-Ali hospital, Zahedan (2010-2011). Zahedan Journal of Research in Medical Sciences 14(8), 74-76.

Shanthi M, Sekar U. 2010. Extended Spectrum Beta Lactamase Producing Escherichia Coli and Klebsiella Pneumoniae: Risk Factors for Infection and Impact of Resistance on Outcomes. SUPPLEMENT TO JAPI, VOL. 58.

Samaha-Kfoury JN, Araj GF. 2003. Recent developments in _ lactamases and extended spectrum _lactamases. British Medical Journal 327, 1209-1213. http://dx.doi.org/10.1136/bmj.327.7425.1209

Shahcheragh IF, Nasiri S, Noveiri H. 2010. The Survey of Genes Encoding Beta-Lactamases, in Escherichia coli resistant to beta-lactam and non-beta- lactam antibiotics. Iranian Journal of Basic Medical Sciences 13, 230-237.

Thomson KS, Sanders CC. 1992. Detection of extended-spectrum β-lactamases in members of the family Enterobacteriaceae: Comparison of the double-disk and three-dimensional tests. Antimicrobial Agents Chemotherapy 36, 1877-1882.

Ullah F, Malik SA, Ahmed J. 2009. Antibiotic susceptibility pattern and ESBLS prevallence in nosocomial Escherichia coli from urinary tract infections in Pakistan. African Journal of Biotechnology 8, 3921-3926.

Varaiya A, Dogra J, Kulkarni M, Bhalekar P. 2008. Extended spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae n diabetic foot infection. Indian Journal of Medical Microbiology 26, 281-282.

Well B, Stood RJ. 1989. The genus Shigella. In Bergy’s manual of systemic bacteriology, 8th ed., Krieg, N.R.; Holt, J.G. and others (eds) Wilkins. Hong Kong, 423-427.