Determination of 96-hr LC50 and lethal responses of Wallago attu to water-borne chromium
Paper Details
Determination of 96-hr LC50 and lethal responses of Wallago attu to water-borne chromium
Abstract
(Importance)Water pollution by heavy metals, especially chromium pollution from industrial source can affect aquatic life, all ecosystems and human health directly or indirectly through food chain. The present research work was conducted under laboratory conditions to determine 96-hr LC50 and lethal responses of a fish, Wallago attu, to water-borne chromium. (Brief Method) The fingerlings of fish were kept in glass aquaria having 35-L water capacity. The toxicity tests were conducted at constant total hardness (250mgL-1), water temperature (28 ºC) and pH (8) with three replications for each test concentration, separately. Physico-chemical parameters of water viz. total hardness, temperature, pH, carbon dioxide, dissolved oxygen, total ammonia, sodium and potassium were monitored twice a day during each 96-hr trial. The 96 hour LC50 and lethal concentrations of chromium, with 95% confidence interval, were computed by using the Probit analysis method. Regression/correlation analyses were also performed to find-out relationships among different variables under study. (Results/key findings) This investigation revealed significant differences between 96-hr LC50 and lethal concentrations of chromium for Wallago attu. The mean 96-hr LC50 and lethal concentrations of chromium for this fish were computed as 59.17±1.45 and 90.65±1.09mgL-1, respectively. (Conclusions)The present investigation also revealed that metallic ion concentrations had significant impacts on the physico-chemical parameters of the test media as total ammonia and carbon dioxide contents showed significant increase with concomitant increase in chromium concentration while dissolved oxygen showed significant decrease. The dissolved oxygen revealed negatively significant relationship with total ammonia. Calcium exhibited statistically highly significant but negative relation with magnesium contents of the test media.
Abedi Z, Khalesi KM, Eskandari KS, Rahmani H. 2012. Comparison of lethal concentrations (LC50 96-hr) of CdCl2, CrCl3 and Pb(NO3)2 in Common Carp (Cyprinus carpio) and Sutchi catfish (Pangasius hypophthalmus). Iranian Journal of Toxicology 6, 672- 680.
Adefemi SO, Asaolu SS, Olaofe O. 2008. Determination of heavy metals in Tilapia mossambicuis fish, associated water and sediment from Ureje Dam in South-Western Nigeria. Research Journal of Environmental Sciences 2, 151- 155. http://dx.doi.org/10.3923/rjes.2008.151.155
American Public Health Association (A.P.H.A). 1998. Standard methods for the examination of water and wastewater. 20th Ed. American Public Health Association, New York, p. 1193.
Bagchi D, Stohs SJ, Downs BW, Bagchi M, Preuss HG. 2002. Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology 180, 5- 22. http://dx.doi.org/10.1016/S0300-483X(02)00378-5
Chakrabarti P, Ghosh SK. 2008. Ultrastructural organisation and functional aspects of the olfactory epithelium of Wallago attu (Bleeker). Folia Morphologica 68, 40- 44.
Dhanalakshmi B. 2013. Acute and chronic toxicity of chromium on biochemical composition of the fresh water major carp Cirrhinus mrigala (hamilton). Asian Journal of Science and Technologies 4, 21- 26.
Domingues I, Oliveira R, Lourenco J, Grisolia KC, Mendo S, Soares MVMA. 2010. Biomarkers as a tool to assess effects of chromium (VI): Comparison of responses in Zebrafish early life stages and adults. Comparative Biochemistry and Physiology 152, 338- 345. http://dx.doi.org/10.1016/j.cbpc.2010.05.010
Farag AM, May T, Marty DG, Easton M, Harper DD, Little EE, Cleveland L. 2006. The effect of chronic Cr exposure on the health of Chinook salmon (Onchorhynchus tshawytscha). Aquatic Toxicology 76, 246- 257. http://dx.doi.org/10.1016/j.aquatox.2005.09.011
Guedenon P, Edorh PA, Hounkpatin YSA, Alimba GC, Ogunkanmi A, Nwokejiegbe GE, Boko M. 2012. Acute Toxicity of chromium (CrCl3) to African Catfish, Clarias gariepinus. Research Journalof Chemical Sciences 2, 41- 45.
Joshi SN, Patil SH. 2002. Effect of water temperature, pH and hardness on the toxicity of chromium trioxide to fish Clarias batrachus. Proc. Indian natnional Science Academy 6, 347- 350.
Kawade SJ, Khillare KY. 2012. Toxicity of chromium on the biochemical contents of certain tissues of freshwater fish, Channa gachua (ham.) International Journal of Applied Biology and Pharmaceutical technology 3, 242- 251.
Khare S, Singh S. 2002. Histopathological lessons induced by copper sulphate and lead nitrate in the gills of fresh water fish Nandus. Journal of Ecotoxicology and Environmental Monitoring 12, 105- 111.
Lushchak OV, Kubrak IO, Nykorak ZM, Storey BK, Lushchak IV. 2008. The effect of potassium dichromate on free radical processes in goldfish: Possible protective role of glutathione. Aquatic Toxicology 87, 108- 114. http://dx.doi.org/10.1016/j.aquatox.2008.01.007.
Malik N, Biswas AK, Qureshi TA, Borana K, Virha R. 2010. Bioaccumulation of heavy metals in fish tissues of a freshwater lake of Bhopal. Environmental Monitoring and Assessment 160, 267- 276. http://dx.doi.org/10.1007/s10661-008-0693-8.
Mijkherjee M, Praharaj A, Das S. 2002. Conservation of endangered fish stocks through artificial propagation and larval rearing technique in West Bengal, India. Aquaculture Asia 7, 8- 11.
Mirza MR. 2003. Check list of freshwater fishes of Pakistan. Pakistan Journal of Zoology Supplement series 3, 1- 30.
Murti R, Omkar, Shukla GS. 2005. Chromium toxicity to a freshwater teleost Channa punctatus. Toxicology Letters 18, 257- 261.
Patra MK, Acharjee SK, Chakraborty SK. 2005. Conservation categories of siluroid fishes in North-East Sundarbans, India. Biodiversity and Conservation 14, 1863- 1876.
Piotrowska A, Mlyni K, Siwek A, Dybala M, Opoka W, Poleszak E, Nowak G. 2008. Antidepressant like effect of chromium chloride in the mouse forced swim test: involvement of glutamatergic and serotonergic receptors. Pharmacological Reports 60, 991- 995.
Prasath PMD, Arivoli S. 2008. Biochemical study of freshwater fish Catla catla with reference to mercury chloride. Iranian Journal of Environmental Health Science and Engineering 5, 109- 116.
Sanjay P, Ravindra K, Shilpi S, Nagpure SN, Satish K, Srivastava S, Verma SM. 2006. Acute toxicity of Cr exposed Channa marulius. Indian Journal of Environmental sciences 38, 118- 121.
Selvam A, Anandhan R, Kavitha V. 2014. Acute toxicity of trivalent chromium to freshwater fish Labeo rohita (ham). International Journal of Research in Fisheries and Aquaculture 4, 104- 107.
Shaukat T, Javed M. 2013. Acute toxicity of chromium for Ctenopharyngodon idella, Cyprinus carpio and Tilapia nilotica. International Journal of Agriculture and Biology 15, 590- 594. http://dx.doi.org/12–589/2013/15–3–590–594
Singh AP, Singh S, Bhartiya P, Yadav K. 2010. Toxic effect of hexavalent chromium on the serum biochemical parameters of snake headed fish Channa punctatus (Bloch). Advanced Biomedical research 1, 177- 181.
Singh D, Singh A. 2002. Biochemical alteration in freshwater fish Channa punctatus. Environmental Toxicology andPharmacology 12, 129- 136. http://dx.doi.org/10.1016/S1382-6689(02)00016-9
Steinhagen D, Helmas D, Maurers S. 2004. Effect of hexavalent carcinogenic chromium on carp, Cyprinus carpio immune cells. Diseases of Aquatic Organisms 23, 155- 161.
Valko M, Morris H, Cronin TM. 2005. Metals toxicity and oxidative stress. Current Medicinal Chemistry 12, 1161- 1208. http://dx.doi.org/0929-8673/05$50.00+.00
Wepener V, Vurer VJHJ, Depreez HH. 2001. Uptake and distribution of a copper, iron and zinc mixture in gill, liver and plasma of a freshwater teleost, Tilapia sparrmani. Water 27, 99- 108.
Sumra Naz, Muhammad Javed, 2019. Determination of 96-hr LC50 and lethal responses of Wallago attu to water-borne chromium. Int. J. Biosci., 14(3), 251-257.
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