Determination of insecticidal resistance in Culex mosquitoes against temephos, cypermethrin and deltamethrin

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Research Paper 01/02/2019
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Determination of insecticidal resistance in Culex mosquitoes against temephos, cypermethrin and deltamethrin

Sadia Abbas, Shabab Nasir, Muhammad Kashif Zahoor, Muhammad Asrar
Int. J. Biosci.14( 2), 18-23, February 2019.
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Pakistan is an agricultural country, so farmers use huge amount of insecticides without discrimination to save their crops. In the mean while they also use those pesticides in their homes for the control of house hold pests. So, this study was carried out to know the status of resistance of temephos and pyrethroids in mosquitoes. For this purpose, mosquitoes were collected, identified and reared in laboratory for bioassay. Larvae were treated in beakers while adults with impregnated papers. In case of temephos, LC50 values from 0.009 to 0.327 ppm were noted  and highest value was found from Lahore mosquitoes that were about 34 fold resistant than susceptible strain. In case of adulticides, cypermethrin showed up to 17.67 fold variation in susceptibility level across all populations while deltamethrin showed up to 33.88 fold variation. The results showed that Lahore mosquitoes were more resistant followed by Rawalpindi and least in case of Faisalabad. Higher level of activity in case of different enzymes like esterase, mixed-function oxidase, glutathione S-transferase and acetyl-cholinesterase were found in resistant populations as compared to susceptible strain. We should use selective chemicals with new mode of action to minimise this problem.


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