Determination of lead biosorption efficacy of soilborne Achromobacter sp. through atomic absorption spectrophotometer (AAS) furnace

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Determination of lead biosorption efficacy of soilborne Achromobacter sp. through atomic absorption spectrophotometer (AAS) furnace

Afrin Priya Talukder, Md. Akhtar-E-Ekram, Md. Nazmul Haque, Shahriar Zaman, Md. Salah Uddin, Md. Abu Saleh
Int. J. Biosci.13( 6), 129-139, December 2018.
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Rapid industrialization has been posed overall environment in jeopardy by discharging huge amount of industrial contaminants, mainly heavy metals into soil and water bodies. Among various heavy metals, lead is a major pollutant and it is necessary to eliminate from environment for minimizing the risk of uptake by plants, animal and human. In view of this, lead contaminated soil sample was collected from industrial area and inoculated in mineral salt (MS) medium supplemented with lead at different concentrations for 5 days for the isolation of bacterial strain. The optimal culture condition of the bacterium was at pH 7.0 and temperature 30°C. Morphological and biochemical properties indicated that the bacterium was gram negative, rod shaped, motile, citrate utilizing, catalase positive and showed negative result for methyl red, urease, starch hydrolysis, mannitol salt agar and lactose fermentation test. Isolated bacterial strain was identified as Achromobacter sp. with 92% homogenity through molecular identification. Isolated Achromobacter sp. was multidrug resistant and showed resistance pattern against cefuroxime, nalidixic acid, cefotaxime, chloramphenicol. Moreover, MIC of gentamycin against the isolated bacterium was determined at 12.5 µg/ml concentration. Lead toxicity was also evaluated against Artemia salina and LC50 value was 2.769± 0.018 mg/ml after 24 hour of exposure which exhibited higher toxicity of lead. Finally, lead biosorption efficiency of the Achromobacter sp. was determined through AAS furnace and highest degradation rate (90%) was observed at 100 μg/ml concentration at day 7 of exposure. Therefore, the study concluded that Achromobacter sp. proved to be a potential biosorbent for the removal of lead from contaminated soil.


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