Determination of lead biosorption efficacy of soilborne Achromobacter sp. through atomic absorption spectrophotometer (AAS) furnace

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Determination of lead biosorption efficacy of soilborne Achromobacter sp. through atomic absorption spectrophotometer (AAS) furnace

Afrin Priya Talukder, Md. Akhtar-E-Ekram, Md. Nazmul Haque, Shahriar Zaman, Md. Salah Uddin, Md. Abu Saleh
Int. J. Biosci.13( 6), 129-139, December 2018.
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Abstract

Rapid industrialization has been posed overall environment in jeopardy by discharging huge amount of industrial contaminants, mainly heavy metals into soil and water bodies. Among various heavy metals, lead is a major pollutant and it is necessary to eliminate from environment for minimizing the risk of uptake by plants, animal and human. In view of this, lead contaminated soil sample was collected from industrial area and inoculated in mineral salt (MS) medium supplemented with lead at different concentrations for 5 days for the isolation of bacterial strain. The optimal culture condition of the bacterium was at pH 7.0 and temperature 30°C. Morphological and biochemical properties indicated that the bacterium was gram negative, rod shaped, motile, citrate utilizing, catalase positive and showed negative result for methyl red, urease, starch hydrolysis, mannitol salt agar and lactose fermentation test. Isolated bacterial strain was identified as Achromobacter sp. with 92% homogenity through molecular identification. Isolated Achromobacter sp. was multidrug resistant and showed resistance pattern against cefuroxime, nalidixic acid, cefotaxime, chloramphenicol. Moreover, MIC of gentamycin against the isolated bacterium was determined at 12.5 µg/ml concentration. Lead toxicity was also evaluated against Artemia salina and LC50 value was 2.769± 0.018 mg/ml after 24 hour of exposure which exhibited higher toxicity of lead. Finally, lead biosorption efficiency of the Achromobacter sp. was determined through AAS furnace and highest degradation rate (90%) was observed at 100 μg/ml concentration at day 7 of exposure. Therefore, the study concluded that Achromobacter sp. proved to be a potential biosorbent for the removal of lead from contaminated soil.

VIEWS 9

Abbas AA, Mohamed SM, Zahir HA. 2016. Biosorption of heavy metals by Pseudomonas bacteria. International Research Journal of Engineering and Technology 3, 1446-1450.

Bandela NN, Satar AG, Tarini M, Geetanjali K. 2016. Heavy metal removal by isolates from domestic and industrial waste water. Journal of Materials and Environmental Science 7, 4589-4595.

Barahona MV, Sánchez-Fortún S. 1999. Toxicity of carbamates to the brine shrimp Artemia salina and the effect of atropine, BW284c51, iso-OMPA and 2-PAM on carbaryl toxicity. Environmental Pollution 104, 469-476.

Bauer AW, Kirby WM, Sherris JC, Turek M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 493-496.

Bennett LE, Burkhead JL, Hale KL, Tery N, Pilon M, Pilon-Smits EAH. 2003. Analysis of transgenic Indian mustard plants for phytoremediation of metal contaminated mine tailings. Journal of Environmental Quality 32, 432–440.

Bergey’s Mannual of Determinative Bacteriology. 1957. Seventh Edition. The Wiliams and Wilkins Company, p 300-301.

Carrasco JA, Armario P, Pajuelo E, Burgos A. 2005. Isolation and characterization of symbiotically effective Rhizobium resistant to arsenic and heavy metals after the toxic spill at the Aznalcollar pyrite mine. Soil Biology and Biochemistry 37, 1131-1140. https://doi.org/10.1016/j.soilbio.2004.11.015

Clarridge JE. 2004. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews 17, 840–862. https://doi.org/10.1128/CMR.17.4.840-862.2004

Cleveland LM, Minter ML, Cobb KA, Scott AA, German VF. 2008. Lead hazards for pregnant women and children, Part 1: immigrants and the poor shoulder most of the burden of lead exposure in this country. American Journal of Nursing 108, 40–49. https://doi.org/10.1097/01.NAJ.0000337736.76730.66

Dopson M, Austin CB, Koppineedi PR, Bond PL. 2003. Growth in sulfidic minerals environments: metal resistance mechanisms in acidophilic micro-organisms. Microbiology 149, 1959-1970. https://doi.org/10.1099/mic.0.26296-0

Edwards BD, Greysson-Wong J, Somayaji R, Waddell B, Whelan FJ, Storey DG, Rabin HR, Surette MG, Parkins MD. 2017. Prevalence and outcomes of Achromobacter species infections in adults with cystic fibrosis: a North American cohort study. Journal of Clinical Microbiology 55, 2074 –2085. https://doi.org/10.1128/JCM.02556-16

Eghomwanre AF, Obayagbona NO, Osarenotor O, Enagbonma BJ. 2016. Evaluation of antibiotic resistance patterns and heavy metals tolerance of some bacteria isolated from contaminated soils and sediments from Warri, Delta State, Nigeria. Journal of Applied Sciences and Environmental Management 20, 287-291. https://doi.org/10.4314/jasem.v20i2.8

Fakayode SO. 2005. Impact Assessment of industrial effluent on water quality of the receiving Alaro river in Ibadan, Nigeria. African Journal of Environmental Assesssment and Management 10, 1-13.

Gajbhiye SN, Hirota R. 1990. Toxicity of heavy metals to brine shrimp Artemia. Journal of the Indian Fisheries Association 20, 43-50.

Ge HW, Lian MF, Wen FZ, Yun YF, Jian FY, Ming T. 2009. Isolation and characterization of the heavy metal resistant bacteria CCNWRS33-2 isolated from root nodule of Lespedeza cuneata in gold mine tailings in China. Journal of Hazardous Materials 162, 50-56. https://doi.org/10.1016/j.jhazmat.2008.05.040

Ikhuoria EU, Okieimen FE. 2000. Scavenging cadmium, copper, lead, nickel and zinc ions from aqueous solution by modified cellulosic sorbent. International Journal of Environmental Studies 57, 401–409. https://doi.org/10.1080/00207230008711284

Institute for Health Metrics and Evaluation (IHME). 2016. GBD Compare. Seattle, WA: IHME, University of Washington.

Jaishankar M, Mathew BB, Shah MS, Gowda KRS. 2014. Biosorption of few heavy metal ions using agricultural wastes. Journal of Environment Pollution and Human Health 2, 1–6.

Järup L. 2003. Hazards of heavy metal contamination. British Medical Bulletin 68, 167-182.

Karrari P, Mehrpour O, Abdollahi M. 2012. A systemic review on status of lead pollution and toxicity in Iran: Guidance for preventive measures. DARU Journal of Pharmaceutical Sciences 20, 2. https://doi.org/10.1186/1560-8115-20-2

Martínez M, Del ramo J, Torreblanca A, Díaz-Mayans J. 1998. Effect of cadmium exposure on zinc levels in the brine shrimp Artemia partenogenética. Aquaculture 172, 315-325.

Nagajyoti PC, Lee KD, Sreekanth TVM. 2010. Heavy metals, occurrence and toxicity for plants: a review. Environmental Chemistry Letters 8, 199–216.

Ray S, Ray Mk. 2009. Bioremediation of heavy metal toxicity-with special reference to chromium. Journal of Medical Sciences 2, 57-63.

Roy A, Queirolo E, Peregalli F, Mañay N, Martínez G, Kordas K. 2015. Association of blood lead levels with urinary F2-8α isoprostane and 8-hydroxy-2-deoxy-guanosine concentrations in first-grade Uruguayan children. Environmental Research 140, 127–135.

Sachiko N, Misaki S, Kana S, Yuki H, Yuki Y, Natsumi G, Hiroo T, Naoto B. 2017. Environmental distribution and drug susceptibility of Achromobacter Xylosoxidans isolated from outdoor and indoor environments. Yonago Acta Medica 60, 67-70.

Sahu RK, Arora NK. 2008. Bioassay as a tool for assessing susceptibility and resistant plant species for field contaminated with industrial effluents. World Journal of Microbiology and Biotechnology 24, 143–148.

Shah SU. 2012. Importance of genotoxicity and guidelines for genotoxicity testing for pharmaceuticals. IOSR Journal of Pharmacy and Biological Sciences 1, 43-54. https://doi.org/10.9790/3008-0124354

Shamsa A, Sikander S. 2016. Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement. Brazilian Journal of Microbiology 47, 563-570. https://doi.org/10.1016/j.bjm.2016.04.009

Singh V, Chauhan PK, Kanta R, DhewaT, Kumer V. 2010. Isolation and characterization of Pseudomonas resistant to heavy metals contaminants. International Journal of Pharmaceutical Sciences Review and Research 3, 165-168.

Solís PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. 1993. A microwell cytotoxicity assay using Artemia salina. Plant Medicine 59, 250-252.

Volesky B. 1994. Advances in biosorption of metals: selection of biomass types. FEMS Microbiology Reviews 14, 291-302.