Paper Details
Determination of physicochemical properties of capsaicin and cytotoxic effect of capsicum extract in breast cancer (MCF7) cell line
Anoosh eghdami, Mahsa Amin Salehi, Mardan Babakhani
DOI: https://dx.doi.org/10.12692/ijb/4.8.262-268
Int. J. Biosci. 4(8), 262-268. April, 2014. (PDF)
Abstract:
Capsaicin (Trans 8methillyl6nonamide) the major pungent ingredient in genus Capsicum, has long been used in food additives and drugs. Capsaicin has been shown to inhibit growth and induce apoptpsis in various transformed cell types in vitro, but not in normal counterpart cells.we investigated about capsaicin in two experimental and computational studies. The medicinal plants were collected, powdered, extracted, lyophilized and kept in 20º C. Breast cancer (MCF7) cell lines and normal connective tissue cell line (L929)were cultured in DMEM medium. Capsicum was extracted; and different dilutions of capsicum extract (1.60 to 1.200) were added to cell culture. Cell viability was quantitated by MTT assay after 24, 48 and 72 hours. The effects of capsicum extract on cell viability were observed after 48 hours on cell lines.capsicum extract doses in dilution 1.100 and 1.70 inhibited 50% cell growth (IC50) in MCF7 cell line after 48hours of incubation, respectively Our study shows that capsicum fresh extract has cytotoxic effects on tumor cells. In this article, theoretical methods have been used for calculation of physical parameters in polyphenols compounds in extract of capsicum. We calculated physical parameters like atomic charges, energy (8Ε), NMR determinan and distance matrix determinant, and in this work we used Gaussian 03at NMR by using HF methods with 321Gbasis set.