Developing rice restorer lines resistant to bacterial blight, blast and brown plant hopper by molecular pyramiding

Paper Details

Research Paper 01/10/2015
Views (292) Download (4)

Developing rice restorer lines resistant to bacterial blight, blast and brown plant hopper by molecular pyramiding

Gandéka Mamadou, Yan Chengye, Ratemo B. Omboki, Wang Xiaojian, Xu Guo Xin, Mo Chong Hong, Tongmin Mou
Int. J. Agron. Agri. Res.7( 4), 155-165, October 2015.
Certificate: IJAAR 2015 [Generate Certificate]


Bacterial blight, blast and brown plant hopper resistance (BB, BL and BPH respectively) genes Xa23, Pi-9, Bph14 and Bph15 were pyramided into an elite restorer line R1005 by using marker assisted selection backcrossing (MABC) and simple sequence repeats (SSR). PCR markers facilitated and accelerated the process of pyramiding of Xa23, Pi-9, Bph14 and Bph15 genes. In this study 75-1-127 harboring Pi9, JYQ9008 harboring Xa23, and B5 harboring Bph14 and Bph15 against resistant bacterial blight, blast and brown planthopper were used as R-genes donor respectively. The pyramided genes and evaluation of agronomic traits related to bacterial blight, blast and brown plant hopper represent best ways in which resistance can be studied. Largest resistance levels were observed against the bacterial blight followed by blast and lastly brown plant hopper pyramid lines. PCR markers for these four genes, Xa23, Pi-9, Bph14, 15 were made available. DNA marker technology was used to identify plants that contained resistant genes to BB, BL and BPH. Restorer lines are known to determine success of pyramided genes. Hence careful background searches should be done before settling on one. Resistant lines can really be the answer to environmental problems that hamper growth of food crops particularly rice and ensure food security for the vulnerable people in society. Pyramiding is considered an ethical way of creating better varieties. The newly variety we created that was resistant to all the three factors had a higher yield than the control. These traits were carried through successive generations as was the case in the hybrid variety.


Amy T, Struedee P. 2010. Novel Strains of Xanthomonas oryzae pv. oryzae UV Mutated Induce Systemic Resistance in Rice against Bacterial Leaf Blight Disease. Kasetstart Journal (Nature Science) 40, 1026-1043.

Basavaraj SH, Singh VK, Singh A, Anand D, Yadav S, Ellur RK, et al. 2010. Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Molecular Breeding 6, 293-305.

Chen QH, Wang YC, Zheng XB. 2006. Genetic diversity of Magnaporthe grisea in China as revealed by DNA fingerprint haplotypes and pathotypes. Journal of Phytopathology 154(6), 361-369.

Chen S, Lin XH, Xu CG, Zhang QF. 2000. Improvement of bacterial blight Resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker assisted selection. Crop Science 239-244.

Chongyun F, Tao W, Wuge L, Feng W, Jinhua L, Xiaoyuan Z, et al. 2012. Genetic improvement of resistance to blast and bacterial blight of the elite maintainer line Rongfeng B in hybrid rice (Oryza sativa L.) by using marker-assisted selection. African Journal of Biotechnology 11(67), 13104-13124.

Fujino K, Sekiguchi H. 2008. Mapping of quantitative trait loci controlling Heading date among rice cultivars in the northernmost region of Japan. Breeding Science 58, 367-373.

Ghulam M, Muhammad MA, Sami UK, Muhammad N, Abdul SM. 2013. Leaf rust resistance in semi dwarf wheat cultivars: a conspectus of post green revolution period in pakistan. Pakistan Journal of Botany 45(SI), 415-422.

Haiyuan Y, Aiqing Y, Zhifan Y, Fu TZ, Ruifeng H, Lili Z. 2004. High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theoretical and Applied Genetics 110, 182-191.

Hsieh SC, Wang LH. 1988. Genetic studies on grain quality in rice. Rice Grain Quality, proc. symposium (pp. 117-136). Taiwan: Taichung District Agricultural Improvement Station.

I H, GA, J, L, P, G, R, E, Y, S, et al. 2012. Genetic analysis of Resistance to Rice Bacterial blight in Uganda. African Crop Science Journal 20.

J Z, X L, G J, Y X, Y H. 2006. Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice. Plant Breeding 125(6), 600-605.

Jie H, Xin L, Changjun W, Changju Y, Hongxia H, Guanjun G, et al. 2012. Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice. Molecular Breeding 29(1), 61-69.

Jung-Pil S, Ji-Ung J, Tae-Hwan N, Young-Chan C, So-Hyun P, Hyun-Su P, et al. 2013. Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice. Rice 6(5).

Q Z, S CL, B YZ, C LW, W CY, Y LZ, et al. 1998. Identification and Tagging a new gene for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Rice genetics 138-141.

Shaoqing L, Daichang Y, Yingguo Z. 2007. Characterization and Use of Male Sterility in Hybrid Rice Breeding. Journal of Integrative Plant Biology 49(6), 791-804.

Sidhu G, Khush G, Mew T. 1978. Genetic analysis of bacterial blight resistance in seventy-four cultivars of rice, Oryza sativa L. Theoretical Applied Genetics 53(3), 105-111.

T O. 1996. Monitoring race distribution and identification of genes for resistance to bacterial leaf blight. Third International Rice Genetics Symposium (pp. 455-459). IRRI.

W P, T T, P T, J S. 2013. Pyramiding of blast and bacterial leaf blight resistance genes into rice cultivar RD6 using marker assisted selection. African Journal of Biotechnology 12(28), 4432-4438.

Yang H, You A, Yang Z, Zhang F, He R, Zhu L, et al. 2004. High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Thoeretical Applied Genetics 110(1), 182-191.

Yong-Li Z, Jian-Long X, Shao-Chuan Z, Jing Y, Xie-wen X, Mei-Rong L, et al. 2009. Pyramiding Xa23 and Rxo1 for resistance to two bacterial diseases into an elite indica rice variety using molecular approaches. Molecular Breeding 23(2), 279-287.

Zhang Q. 2005. Highlights in Identification and Application of Resistance Genes to Bacterial Blight. Chinese Journal of Rice Science 19(5), 453-459.

Zhu X, Yang Q, Yang J, Lei C, Wang J, Ling Z. 2004. Differentiation ability of monogenic lines to Magnaporthe grisea in indica rice. ACTA Phytopathologica sinica 4, 361-368.