Development and validation of a teratogenicity of golden oyster mushroom (Pleurotus citrinopileatus Singer.) extract on Zebrafish (Danio rerio)

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Research Paper 15/11/2022
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Development and validation of a teratogenicity of golden oyster mushroom (Pleurotus citrinopileatus Singer.) extract on Zebrafish (Danio rerio)

Justin V. Dumale, Geraldine R. Gamoso
Int. J. Biosci.21( 5), 227-231, November 2022.
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Mushrooms are regarded as a rich source of nutritive components; yet, despite their high potential for bioactive chemicals, mushrooms can have harmful effects on developing organisms such as cytotoxicity and teratogenicity. This article examined the development and teratogenic effects of this mushroom on zebrafish (Danio rerio) as an animal model in order to determine its biosafety and potential as a source of chemicals with bioactivities. In order to get functional components for teratogenic and toxic effects in zebrafish embryos, hot water extraction was performed. Extract dramatically decreased the hatchability of zebrafish eggs at 1% or higher concentrations of water extract, as well as the heartbeat rate at 10% or higher concentrations. Embryos treated to a 5% concentration of the extract had growth retardation, which coagulated following treatment. Lethality was detected in the form of a coagulated embryo and slowed development. The biological activity of P. citrinopileatus provides information on its possible use as a bioactive chemical source. As a result, more research into other features is deemed important in future studies.


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