Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | August 1, 2021

| Download 24

Development of conservation techniques for liver samples from rodents of the genus Rattus in Abidjan

Ambroise Kouamé Kintossou, Stanislas Egomli Assohoun, Lydie Amon, Marcelle Money, Stéphane Kan Kouassi, Souleymane Cissé, Joseph Allico Djaman, Amadou Founzégué Coulibaly, Mireille Dosso

Key Words:

Int. J. Biosci.19(2), 36-50, August 2021

DOI: http://dx.doi.org/10.12692/ijb/19.2.36-50


IJB 2021 [Generate Certificate]


Tissue samples should be stored in liquid nitrogen immediately after excision and/or kept at -80°C until analysis. In relation to this requirement, researchers in Côte d’Ivoire have difficulty collecting and transporting tissue samples to areas far away from testing laboratories due to the unavailability, use and cost of preservation equipment. In this study, we prepared two preservation solutions and evaluated their ability to protect liver tissue RNAs for two months for collection and transport to a testing laboratory. Two preservative solutions named solution S1 and S2 were prepared to perform this experimental study. As an animal model for the experiment, we used 15 rats of the genus Rattus. These rats were sacrificed and liver tissue samples were collected and aliquoted according to the solutions, temperatures and storage times. A total of 1500 samples were analysed. Liver RNA can be stored without solution at +18°C for 6 hours, at +4°C for 3 days and at -20°C for 5 days. Solutions S1 and S2 have been shown to preserve liver RNA for 12 hours at +18°C, for 3 days at +4°C and for 30 days at -20°C. Therefore, solution S2 can preserve liver RNA for up to 60 days at -20°C in contrast to solution S1 which does so for 30 days at the same temperature. Solution S2 could be indicated for the preservation of liver RNA for 2 months.


Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Development of conservation techniques for liver samples from rodents of the genus Rattus in Abidjan

Anna Almeida, Jean Paul Thiery, Henri Magdelénat, François Radvanyi. 2004. Gene expression analysis by real-time reverse transcription polymerase chain reaction: inXuence of tissue handling. Analytical Biochemistry 328, 101–108.

Bao WG, Zhang X, Zhang JG, Zhou WJ, Bi TN, Wang JC, Yan WH, Lin A. 2013. Biobanking of fresh frozen human colon tissues: impact of tissue ex-vivo ischemia times and storage periods on RNA quality. Annals of Surgical Oncology 20, 1737–44.

Barnes RO, Parisien M, Murphy LC, Watson PH. 2008. Influence of evolution in tumor biobanking on the interpretation of translational research. Cancer Epidemiol, Biomarkers & Prevention 17, 3344–50.

Bray SE, Paulin FE, Fong SC, Baker L, Carey FA, Levison DA, Steele RJC, Kernohan NM. 2010. Gene expression in colorectal neoplasia: modifications induced by tissue ischaemic time and tissue handling protocol. Histopathology 56, 240–50.

Compagnon Philippe. 2011. Solutions de conservation du greffon hépatique. Liver preservation solutions. Le Courrier de la Transplantation – Vol. XI – n° 4 – octobre-novembre-décembre 2011.

De Cecco L, Musella V, Veneroni S, Cappelletti V, Bongarzone I, Callari M, Valeri B, Pierotti MA, Daidone MG. 2009. Impact of biospecimens handling on biomarker research in breast cancer. BMC Cancer 9, 409.

Den Toom D, De Jong M, Krenning EP, Van der Hoek HJ, Ten Kate FJ, Hennemann G, Terpstra OT. 1991. Euro-collins solution versus uw solution for long-term liver preservation in the isolated rat-liver perfusion model. Hepato-Pancreato-Biliary Surgery 4, 313-20.

Drinkwater DC, Rudis E, Laks H, Ziv E, Marino J, Stein D, Ardehali A, Aharon A, Moriguchi J, Kobashigawa J. 1995. University of Wisconsin solution versus Stanford cardioplegic solution and the development of cardiac allograft vasculopathy. Journal of Heart and Lung Transplantation 14, 891-6.

Hong SH, Baek HA, Jang KY, Chung MJ, Moon WS, Kang MJ, Lee DG, Park HS. 2010. Effects of delay in the snap freezing of colorectal cancer tissues on the quality of DNA and RNA. Journal of the Korean Society Coloproctology 26, 316–23.

Hood BL, Conrads TP, Veenstra TD. 2006. Unravelling the proteome of formalin-fixed paraffinembedded tissue. Briefings in Functional Genomics and Proteomics 5, 169–175.

Jerry JL, Leili M, Desiree ES, Ryan WW, Maryam S, Gregory ML, Bowen W, Elyse JS, Sergey M, William HY. 2014. A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories. Clinical Biochemistry. 47(0), 267–273. http://dx.doi.org/10.1016/j.clinbiochem.2013.12.011.

Jiang A, Liu C, Liu F, Song YL, Li QY, Yu L, Lv Y. 2012. Liver cold preservation induce lung surfactant changes and acute lung injury in rat liver transplantation. World Journal of Gastroenterology 18, 323-30.

Lou JJ, Mirsadraei L, Sanchez DE, Wilson RW, Shabihkhani M, Lucey GM, Wei B, Singer EJ, Mareninov S, Yong WH. 2014. A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories. Clinical Biochemistry 47(4-5), 267-273. http://dx.doi.org/10.1016/j.clinbiochem.2013.12.011

Micke P, Ohshima M, Tahmasebpoor S, Ren ZP, Ostman A, Pontén F, Bloting J. 2006. Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens. Laboratory Investigation 86, 202–11.

Naber SP, Smith LL Jr, Wolfe HJ. Role of the frozen tissue bank in molecular pathology. 1992. Diagnostic Molecular Pathology 1, 73–79.

Naber SP. 1996. Continuing role of a frozen-tissue bank in molecular pathology. Diagnostic Molecular Pathology 5, 253–259.

Rosenfeldt FL, Conyers RA, Jablonski P, Langley L, Richards SM, Self G, Marshall V. 1996. Comparison of UW solution and St. Thomas’ solution in the rat: Importance of potassium concentration. Annals of Thoracic Surgery 61, 576-84.

Sampaio-Silva F, Magalhães T, Carvalho F, Dinis-Oliveira RJ, Silvestre R. 2013. Profiling of RNA degradation for estimation of post mortem interval. PLoS One 8, e56507.

Srinivasan M, Sedmak D, Jewell S. 2002. Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids. American Journal of Pathology 161, 1961–1971.

Swash M, Schwartz MS. 1984. Laboratory methods. In: Swash M, Schwartz MS, editors. Biopsy Pathology of Muscle, 1st edn. London: Chapman and Hall, p 14-8.

Van Maldegem F, de Wit M, Morsink F, Musler A, Weegenaar J, Van Noesel CJ. 2008. Effects of processing delay, formalin fixation, and immunohistochemistry on RNA recovery from formalin-fixed paraffin-embedded tissue sections. Diagnostic Molecular Pathology 17, 51–8.

von Heesen M, Seibert M, Hülser M, Scheuer C, Wagner M, Menger MD, Schilling MK, Moussavian MR. 2012. Multidrug donor preconditioning protects steatotic liver grafts against ischemia-reperfusion injury. American Journal of Surgery 203, 168-76.

Yasemin GK, Bulent K, Omer O, Turgut T, Abdullah K, Tuba M, Cengizhan A, Kenan S, Fatih S, Nuri Y, Ibrahim A, Semih A, Safak E. 2015. Preservative solution for skeletal muscle biopsy samples. Annals of Indian Academy of Neurology 18, 187-193.