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Dietary krill oil (Euphausia superba) alleviates oxidative stress and DNA damages bio-markers in an experimental model of cafeteria diet-induced insulin resistance in rats

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Research Paper 01/05/2017
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Dietary krill oil (Euphausia superba) alleviates oxidative stress and DNA damages bio-markers in an experimental model of cafeteria diet-induced insulin resistance in rats

Zoheir Mellouk, Maria Agustina, Jose Arivalo
Int. J. Biosci.10( 5), 199-206, May 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: Cafeteria dietDNA oxidative damageDyslipidemiaEuphausia superbaInsulin resistanceLipid peroxidationRats

Abstract

Chronic exposure to cafeteria-based diet has been shown to exert a number of adverse metabolic effects in both human and experimental studies. Indeed, krill oil has been the subject of extensive investigation regarding its possible beneficial effects on insulin resistance-related disorders. The present experiment aims to investigate the therapeutic effect of krill oil (Euphausia superba) in the modulation of metabolic disorders, oxidative stress and DNA oxidative damage markers in an experimental model of cafeteria diet-induced insulin resistance. A total of 30, 8-week male Wistar rats were divided into three equal groups: the control diet group (Control), the cafeteria diet group (CAF), and the cafeteria diet group enriched with krill oil at 2% (CAF-KO). After 8 weeks of the experiment, weight gain, adiposity index as well as plasma glucose, insulin, cholesterol and triglycerides were assayed. Insulin resistance was estimated using homeostasis model assessment (HOMA). In parallel, plasma and target tissues’ (liver, pancreas, adipose tissue and muscle) pro-oxidant status were assessed by assaying thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LPO), and isoprostanes (8-isoprostanes). The DNA oxidative damage was evaluated through measurement of the main product of its oxidation (8-OHdG). The 8-week cafeteria diet led to obesity, insulin resistance and dyslipidemia. Furthermore, this obesogenic diet increased the metabolic response to radical attack and DNA oxidation in both plasma and key tissues. Dietary krill oil displays remarkable health benefits by improving insulin resistance and dyslipidemia, which acts by preserving antioxidant mechanisms and protecting cellular components such as DNA and lipids from oxidative damage.

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