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Research Paper | November 1, 2013

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Differentially expressed proteins in suspension culture of rice induced by blast disease

A. Kahe1, M. Toorchi, E. Dorani1, S. Aharizad, M. Arzanlo

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Int. J. Biosci.3(11), 189-197, November 2013

DOI: http://dx.doi.org/10.12692/ijb/3.11.189-197


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Rice (Oryza sativa L.) is an important cereal for more than half of the human population in the world. Magnaporthe grisea is the agent of the most important rice fungus diseases in the world. This experiment was conducted to investigate the effect of blast fungus on expression of proteins in suspension culture of rice. Suspension culture of rice inoculated with conidial suspension (1105 conidia/mL) races of M. grisea for 48 hours. For identification of proteins involved in resistance to magnaporth disease, proteins profile of suspension culture was investigated after inoculation. Comparing proteome pattern of susceptible rice cultivar, Tarom, under control and stress conditions lead to classification differentially expressed proteins into two functional categories, defense and metabolism/biosynthesis. Metabolism and biosantesis related proteins include triose phosphate isomerase, Isoflavone reductase and Transketolase were as the most important defense protein was pathogen related protein.


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