Discrimination of alfalfa half-sib families by allozyme banding pattern and its relationship with forage yield attributes

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Research Paper 01/01/2015
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Discrimination of alfalfa half-sib families by allozyme banding pattern and its relationship with forage yield attributes

Hossein Mohammadzadeh Jalaly, Mostafa Valizadeh, Maryam Ahmadi, Halleh Nabizadeh, Sajjad Moharramnejad, Mohammad Moghaddam
J. Biodiv. & Environ. Sci. 6(1), 344-350, January 2015.
Copyright Statement: Copyright 2015; The Author(s).
License: CC BY-NC 4.0

Abstract

Electrophoretic variation of three allozyme systems coding by seven gene loci were studied on 12 alfalfa half-sib families. Obtained from alfalfa polycross nursery performed in Faculty of Agriculture, University of Tabriz. Polyacrylamide gel electrophoresis was used to evaluate the levels of genetic variations and population structure. The level of polymorphic loci (P) in families was 0.571 and observed mean heterozigosities (Ho) were ranged from 0.59 in Selvana to 0.77 in Galebani half-sibs. Expected mean heterozygosities (He) were ranged from 0.332 in Selvana to 0.439 in Chaleshte. The families were found to be in Hardy-Weinberg equilibrium by using x2 test. Wright’s F statistics revealed that the estimated overall inbreeding coefficient, (FIT), of 0.091 was mainly related to inbreeding or double reduction in alfalfa (FIS= 0.078) rather than random genetic drift or population differentiation (FST= 0.013). The mean of distance coefficient in families were ranged from 0.94 to 0.99 and the lowest distance obtained for Chaleshte and Galebani. The presence of esterase allozyme of Est-b1 was recognized to be related to the fresh weight and leaf weight and peroxidase allozyme Pox-b2 band was correlated significantly to the fresh weight in the studied half-sib families.

Anonymous. 2007. Agricultural Statistics. Information and Statistical Center, Ministry of Jihad-Keshavarzi (Agriculture), Teheran, Iran.

Bahar M, Ghobadi S, Erfani Moghaddam V, Yamchi A, Talebi Bedaf M, Kaboli MM, Mokhtarzadeh AA. 2006. Evaluating genetic diversity of Iranian alfalfa local populations using expressed sequence tags (ESTs) microsatellites. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 10, 141-154.

Brummer EC,  Kochert  G,  Bouton JH.  1991. RFLP variation in diploid and tetraploid alfalfa. Theoretical and Applied Genetics 88, 89-96.

Charmet G, Balfourier F. 1994. Isozyme variation and species relationships in the genus Lolium L. (ryegrasses, Graminaceae). Theoretical and Applied Genetics 87, 641–649.

Cordero A, Crespo M. 1995. Caracterización del ecotipo de alfalfa Tierra de Campos. Pastos, XXV, 57-86.

Flajoulot S, Ronfort J, Baudouin P, Barre P, Huguet T, Huyghe C, Julier B. 2005. Genetic diversity among alfalfa (Medicago sativa L.). cultivars coming from a breeding program, using SSR markers. Theoretical and Applied Genetics 111, 1420-1429.

Harris H, Hopkinson DA. 1976. Handbook of enzyme electrophoresis in human genetics. North-Holland Publishing Company.

Hunter H, Markert CL. 1957. Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science 125, 1294-1295.

Kahler AL. 1983. Elect of half-sib and S1 recurrent selection for increased grain yield on allozyme polymorphisms in maize (Zea mays L). Crop Science 23, 572576.

Li P, Wang Y, Sun X, Han J. 2009. Using microsatellite (SSR) and morphological markers to assess the genetic diversity of 12 falcata (Medicago sativa spp. falcata) populations from Eurasia. African Journal of Biotechnology 8, 2120-2108.

Markert CL, Moller F. 1959. Multiple forms of enzymes: tissue, ontogenetic and species specific patterns. Proceedings of the National Academy of Sciences 45, 753-763.

Mengoni A, Gori A, Bazzicalupo M. 2000. Use of RAPD and Microsatellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa (Medicago sativa). Plant Breeding 119, 311-317.

Morales Corts MR, Crespo Martinez MC. 2000. Variation of PGM and IDH isozymes for identification of alfalfa varieties. Euphytica 112, 137–143.

Muslera P, Ratera C. 1984. Praderas y Forrajes. Ediciones Mundi-Prensa. Madrid. (España), pp 625– 695.

Nei M. 1972. Analysis of gene diversity in subdivided populations Proceedings of the National Academy of Sciences 70, 3321-3323.

Nevo E. 1978. Genetic variation in natural populations: Patterns and theory. Theoretical Population Biology 13, 121-177.

Nielsen G, Ostergaard H, Johansen H. 1985. Cultivar identification by means of isozymes II. Genetic variation at four enzyme loci in diploid Rye grass. Plant Breeding 94, 74–86.

Noparvar S, Valizadeh M, Monirifar H, Razban Haghighi H, Darbani B. 2008. Genetic diversity among and within alfalfa populations native to Azerbaijan based on RAPD analysis. Journal of Biological Research 10, 159-169.

Olson PD, Varner JE. 1993. Hydrogen peroxides and lignification. Plant Journal 4, 887-892.

Pasteur N, Pasyeur G, Bonhomme F, Catalan J, Britton-Davidian J. 1988. Genetic interpretation of gels. In: Practical Isozyme Genetics. Johhn Wiley and Sons, Ellis Horwood, pp 31-48.

Pham JL. 1988. Isozyme markers for plant height and spikelet fertility in rice. Rice Genetics Newsletter 5, 108- 109.

Pupilli F, Businelli S, Paolocci F, Scotti C, Damiani F, Arcioni S. 1996. Extent of RFLP variability in tetraploid population of alfalfa (Medicago sativa). Plant Breeding 115, 106-112.

Quiros CF. 1980. Identification of alfalfa plants by enzyme electrophoresis. Crop Science 20, 262-264.

Quiros CF. 1981. Starch gel electrophoresis technique used with alfalfa and other Medicago species. Canadian Journal of Plant Science 6, 745-749.

Quiros CF. 1983. Perennials, Alafalfa and its closely related species. In: Tanksley SD, Orton TJ. (Ed.), Isozymes in plant genetics and breeding. Part B. Elsevier, Amesterdam, pp 253-294.

Quiros CF, Kerbi K. 1982. Determination by allozymes of natural cross-pollination and hibridization in alfalfa. Plant breeding 89, 177–186.

Sinskaya GN. 1950. Flora of Cultivated Plants of USSR XIII, Perennial Leguminous Plants. Part I: Medic, Sweet Clover. Fenugreek, Moscow.

Soltis DE, Soltis PS. 1990. Isozymes in Plant Biology. Chapman and Hall, London, pp 259.

Stuber CW, Moll RH. 1972. Frequency changes of isozyme alleles in a selection experiment for grain yield in maize. Crop Science 12, 337-340.

Stuber CW, Moll RH, Goodman MM, Schaler H, Weir BS. 1980. Allozyme frequency changes associated with selection for increased grain yield in maize. Genetics 95, 225-236.

Valizadeh M, Moghaddam M, Talebi P, Kazemi MH, Monirifar H, Hassanpanah D. 2002. Breeding and Introduction of suitable alfalfa cultivars in East-Azarbaijan. University of Tabriz Research Affairs Pub., Tabriz, Iran, pp 120.

Valizadeh M, Mohayeji M, Yasinzadeh N, Nasrullazadeh S, Moghaddam M. 2011. Genetic diversity of synthetic alfalfa generations and cultivars using tetrasomic inherited allozyme markers Journal of Agricultural Science and Technology 13, 425-430.

Van DX, Jin LD. 2011. Relationship between esterase isozymes and some agronomic traits in F2 populations derived from the crossing of Milyang 23 and Ashahi. Journal of Crop Science and Biotechnology 14, 11-15.

Wright S. 1951. The genetical structure of populations. Annals of Eugenics 15, 323–354.

Yeh FC, Yang R. 1999. Microsoft Window-Based Freeware for Population Genetic Analysis (POPGENE version 1.31). University of Alberta, Edmonton, Canada.

Zhi-Peng L, Gongshe L, Yang QC. 2007. A novel statistical method assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.). Genetics and Molecular Biology 30, 385-391.

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