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Discrimination of alfalfa half-sib families by allozyme banding pattern and its relationship with forage yield attributes

Hossein Mohammadzadeh Jalaly, Mostafa Valizadeh, Maryam Ahmadi, Halleh Nabizadeh, Sajjad Moharramnejad, Mohammad Moghaddam

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J. Bio. Env. Sci.6(1), 344-350, January 2015

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Abstract

Electrophoretic variation of three allozyme systems coding by seven gene loci were studied on 12 alfalfa half-sib families. Obtained from alfalfa polycross nursery performed in Faculty of Agriculture, University of Tabriz. Polyacrylamide gel electrophoresis was used to evaluate the levels of genetic variations and population structure. The level of polymorphic loci (P) in families was 0.571 and observed mean heterozigosities (Ho) were ranged from 0.59 in Selvana to 0.77 in Galebani half-sibs. Expected mean heterozygosities (He) were ranged from 0.332 in Selvana to 0.439 in Chaleshte. The families were found to be in Hardy-Weinberg equilibrium by using x2 test. Wright’s F statistics revealed that the estimated overall inbreeding coefficient, (FIT), of 0.091 was mainly related to inbreeding or double reduction in alfalfa (FIS= 0.078) rather than random genetic drift or population differentiation (FST= 0.013). The mean of distance coefficient in families were ranged from 0.94 to 0.99 and the lowest distance obtained for Chaleshte and Galebani. The presence of esterase allozyme of Est-b1 was recognized to be related to the fresh weight and leaf weight and peroxidase allozyme Pox-b2 band was correlated significantly to the fresh weight in the studied half-sib families.

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Discrimination of alfalfa half-sib families by allozyme banding pattern and its relationship with forage yield attributes

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