Welcome to International Network for Natural Sciences | INNSpub

Distribution and diversity Ralstonia solanacearum wilt disease bacterial causes of banana (Kepok: Local Indonesia) and intensity of attack in South Kalimantan, Indonesia

Research Paper | August 1, 2017

| Download 3

Yusriadi, A. L. Abadi, S. Djauhari, H. Halim

Key Words:

J. Bio. Env. Sci.11( 2), 78-83, August 2017


JBES 2017 [Generate Certificate]


Ralstonia solanacearum is a bacterial wilt disease in South Kalimantan, Indonesia.  These bacteria have been scattered in banana (kepok: local Indonesia) planting which is a superior commodity, with a loss rate of almost 70-80%.  Since 2007 the losses due to this disease have increased until mid-2014, the high level  loss of resulted 100% .  The purpose of this research was to determine the extent of the strain diversity and distribution of these bacteria extensively both of which attacked bananas, as well as alternative host plants in South Kalimantan.   Methods used (1) Field surveys to districts that planted many banana and bacterial wilt disease, dyeing test on sterile water to ascertain the cause, (2) The sampling of plants attacked by climber and plant samples around the bacterial wilt disease, identification on nutrient agar (NA) and Triphenyl tetrazolium chaloride  (TZC).  Results that the bacteria were found in all districts in South Kalimantan, with no different diversity, the appearance of bacteria on NA and TZC, showed a round shape colony with serrated, middle red and clear surrounding edges. Distribution of bacteria ranging from the district of Banjar, Tanah Laut, Banjarbaru, Tapin and to the farthest district Tabalong and Kotabaru.   The highest level of attacks in the districts of Banjar, Banjarbaru and Kotabaru (100%).  Other host plants found as many as 8 species in Banjar and Banjarbaru.  Conclusion of the distribution of this bacteria is evenly distributed in South Kalimantan, the level of strain diversity is not different and found many alternative host plants.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Distribution and diversity Ralstonia solanacearum wilt disease bacterial causes of banana (Kepok: Local Indonesia) and intensity of attack in South Kalimantan, Indonesia

Álvarez B, Vasse J, Le-Courtois V, Trigalet-Démery D, López MM, Trigalet A. 2008. Comparative behavior of Ralstonia solanacearum biovar 2 in diverse plant species. Phytopathology 98, 59-68. http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-98-1-0059

Djaya AA. 1994.  Efforts of Bacterial Late Control (Pseudomonas solanacearum E. F. Smith) On Ginger with Microorganisms Antagonists, Seed and Land Treatment. Thesis Prog. Postgraduate IPB, Bogor.

Hayward AC.  1990.  Diagnosis, Distribution and Status of Groundnut Bacterial Wilt.  In Middleton & Hayward (Eds.). Proceeding of an ACIAR/ICRISAT collaborative research planning meeting held at Genting Highlands, Malaysia 1990.  ACIAR Proceedings 31, 12-17.

Hayward AC. 1994. The host of Pseudomonas solanacearumIn Hayward, A.C. & G.L. Hartman (Eds.).  Bacterial Wilt, the Disease and its Causative Agent P. solanacearum.  CAB Int., U.K.:9-24.

Hartman GL, Hong, Hanudin WF, Hayward AC. 1993.  Potensial of Biological and Chemical Control of Bacterial Wilt.   In Hartman, G.L. & A.C Hayward (Eds).  Bacterial Wilt.  Proc. of an international conference held at Kaohsiung, Taiwan, October 1992.  ACIAR Proceedings No.45:322-326.

Horita M, Tsuchiya K. 2001. Genetic Diversity of Japanese Strain of Ralstonia solanacearum. APS Press. 91(4), 399-407.

Jaunet TX, Wang JF. 1999. Variation in genotype and aggres-siveness diversity of Ralstonia solanacearum race1 isolated from tomato in Taiwan. Phytopathology 89, 320-327. http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.4.320

Ji Pingshen, Allen C, Sanchez-Perez A, Yao J, Elphinstone JG, Jones JB, Momol MT. 2007. New Diversity of Ralstonia solanacearum Strains Associated with Vegetable and Ornamental Crops In Florida. Plant Disease 91, 195-203. http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-91-2-0195

Fahy EM, Persley GJ.  1983.  Plant Bacterial Disease a Diagnostic Guide. Academic Press. Australia. page303.

Machmud M.  1986.  Bacterial wilt in Indonesia.  In Persley G.J. (Ed).  Bacterial Wilt Disease in Asia and the South Pacific.  Proc. Of an Int.  Workshop held at PCARRD-ACIAR, Philippines. ACIAR Proceedings 13, 32-34.

Machmud M.  1989.  Resistance of Varieties and Plasma of Nutfah Peanut to Lute Disease (Pseudomonas solanacearum). In Sham, Mahyudin (ed) Seminar on Bogor Food Crops Research. 471-482.

Suryadi Y, Machmud M. 2004. Advances in Detection and Identification Techniques of Pseudomonas solanacearum. Journal of Scientific Review of Biological Research and Agricultural Biotechnology 1(1).

Yabuuchi E, Osaka Y, Yano I, Hotta H. Nishiuchi Y.  1995.  Transfer of two Burkholderia and Alcali Genes Spesies to Ralstonia Gen; Proposal of Ralstonia picketti (ralston, palleroni and Doudroff, 1973) Comb., Ralstonia solanacearum (Smith, 1986) Comb.  Nov.  and Ralstonia eutropha (Davis, 1969) Com. Nov.

Yusriadi B, Tjahjono M, Sinaga S, Machmud M. 1998. The Influence of Giving Antagonist Mikoorganisme (P. fluorescens and Trichoderma spp.) To the development of Bacterial Lands Disease (P. solanacearum E.F. Smith) peanut plant. Bulletin of IPT IPT. 9(2).

Yusriadi A, Abadi L, Halim H, Djauhari S. 2013.  Growth Pattern of Ralstonia Solanacearum Bacteria Causes of Lute Disease In South Kalimantan. Proceedings of the 22nd National Seminar of Indonesian Biological Society. Purwokerto, Indonesia