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Diverse antioxidative effects in Pui vegetable (Basella alba) induced by high temperature stress

M.M. Islam, M.S. Haque, M.K. Hossain, M.M. Hasan

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Int. J. Agron. Agri. Res.5(5), 135-147, November 2014


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Basella alba is a green vegetable and grows in both winter and summer; however the temperature sensitivity on metabolic regulation in this species is not clarified. To identify the physiological mechanisms involved in regulation of adaptive response and anti oxidative effects in leaves of Basella alba, plants grown in pot were exposed to high temperature (45 oC) for 24h, 48h and 72h periods and polyphenol oxidase (PPO) and peroxidase (POD) activities in leaves were examined. High temperature causes the higher activity of PPO and the activity was found to be potential after prolonged exposure when compared to the respective controls. Dose response characteristics of substrate on PPO activity were performed. The activity was higher at 10 mM catechol, substrate for the enzyme, than 100 mM and 200 mM concentration, however, the three doses yielded the gradual increase in activity. Conversely, POD activity in leaf was regulated reciprocally and found to be prevented up to 72h of treatment however the effects were appeared to be pronounced after 48h of exposure. The above findings demonstrate that assay of PPO and POD in response to high temperature is an index for characterization of anti oxidative effects in this species of plant and will give a new insight for adaptive response to the adverse environment.


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Diverse antioxidative effects in Pui vegetable (Basella alba) induced by high temperature stress

Ahmadi G, Zienaly Khane Ghah H, Rostamy MA, Chogan R. 2000. The study of drought tolerance and biplot method in eight corn hybrids. Iran. Journal of Agricultural Science 31, 513-523.

Abu-Khadejeh A, Shibli R, Makhadmeh I, Mohammad M. 2012. Influence of increased salinity on physiological responses of hydroponic grown tomato (Lycopersicon esculentum Mill.). Jordan Journal of Agricultural Sciences 8(3), 321-331.

Almeselmani M, Deshmukh PS, Sairam RK, Kushwaha SR, Singh TP. 2006. Protective role of antioxidant enzymes under high temperature stress. Plant Science 171, 382-388.

Boullier A, Bird DA, Chang MK, Dennis EA, Friedman P, Gillotre-Taylor K, Horrko S, Palinski W, Quehenberger O, Shaw P, Steinberg D, Terpstra V, Witztum JL. 2001. Scavenger receptors, oxidized LDL, and atherosclerosis. Annals of the New York Academy of Sciences 947, 214-222.

Bowler C, Montagu MV, Inze D. 1992. Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43, 83-116.

Chisari M, Barbagallo RN, Spagna G. 2007. Characterization of polyphenol oxidase and peroxidase and influence on browning of cold stored strawberry fruit. Journal of Agricultural and Food Chemistry 55(9), 3469-3476.

Chen EL, Chenm YA, Chen LM, Liu ZH. 2002. Effect of copper on peroxidase activity and lignin content in Raphanus sativus. Plant Physiology and Biochemistry 40(5), 439-444.

Cushman JC, Bohnert HJ. 2000. Genomic approaches to plant stress tolerance. Current Opinion in Plant Biology 3, 117-124.

Chaitanya KV, Sundar D, Reddy AR. 2001. Mulberry leaf metabolism under high temperature stress. Biologia Plantarum 44, 379-384.

Daz J, Bernal A, Pomar F, Merino F. 2001. Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Science 161(1), 179-188.

Gawlik-Dziki U, Zlotek U, Swieca M. 2008. Characterization of polyphenol oxidase from butter lettuce (Lactuca sativa var. capitata L.). Food Chemistry 107(1), 129-135.

Jiang YM, Duan XW, Joyce D, Zang ZQ, Li JR. 2004. Advances in understanding of enzymatic browning in harvested litchi fruit. Food Chemistry 88(3), 443-446.

Janska A, Marsik P, Zelenkova S, Ovesna J. 2010. Cold stress and acclimation- what is important for metabolic adjustment? Plant Biology 12(3), 395-405.

Kwak SS, Kim SK, Park IH, Liu JR. 1996. Enhancement of peroxidase activity by stressed-related chemicals in sweet potato. Phytochemistry 43(3), 565-568.

Lee MK, Park I. 2007. Studies on inhibition of enzymatic browning in some foods by Du-Zhong (Eucommia uimoides Oliver) leaf extract. Food Chemistry 114, 154-163.

Lee DH, Lee CB. 2000. Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: In gel enzyme activity assays. Plant Science 159(1), 75-85.

Muftugil N. 1985. The peroxidase enzyme activity of some vegetables and its resistance to heat. Journal of the Science of Food and Agriculture 36, 877-880.

Mahadevan A, Sridhar R. 1982. Methods in Physiological Plant Pathology. 2nd ed. Sivakami Publications, Madras, India, 316, p.

Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics 444, 139-158.

Makoi JHJR, Ndakidemi PA. 2007. Biological, ecological and agronomic significance of plant phenolic compounds in rhizosphere of the symbiotic legumes. African Journal of Biotechnology 6(12), 1358-1368.

Muñoz O, Sepúlveda M, Schwartz M. 2004. Effects of enzymatic treatment on anthocyanin pigments from grapes skin from Chilean wine. Food Chemistry 87(4), 487-490.

Oidaira H, Satoshi S, Tomokazu K, Takashi U. 2000. Enhancement of antioxidant enzyme activities in chilled rice seedlings. Plant Physiology 156, 811-813.

Premalatha B, Rajgopal G. 2005. Cancer- an ayurvedic perspective. Pharmacological Research 51, 19-30.

Queiroz C, Lopes MLM, Fialho E, Valente-Mesquita VL. 2008. Polyphenol oxidase: Characteristics and mechanisms of browning control. Food Reviews International 24(4), 361-375.

Roshan A, Naveen, KHN, Shruthi SD. 2012. A review on medicinal importance of Basella alba L. International Journal of Pharmaceutical Sciences and Drug Research 4(2), 110-114.

Scandalios JG. 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Brazilian Journal of Medical and Biological Research 38(7), 995-1014.

Siers H. 1991. Oxidative stress: from basic research to clinical application. American Journal of Medicine 91, 31-38.

Thypyapong P, Hunt MD, Steffens JC. 1995. Systemic wound induction of potato (Solanum tuberosum) polyphenol oxidase. Phytochemistry 40(3), 673-676.

Vamos-Vigyazo L. 1981. Polyphenoloxidase and peroxidase in fruits and vegetables. Critical Reviews in Food Science and Nutrition 15, 49-126.

Wang W, Vinocur B, Altman A. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218(1), 1-14.

Yadegari LZ, Heidari R, Carapetian J. 2007. The influence of cold acclimation on proline, malondialdehyde (MDA), total protein and pigments contents in soybean (Glycine max) seedlings. Journal of Biological Sciences 7(8), 1436-1441.

Ye S, Yo-Xin Y, Heng Z, Yuan-Peng D, Feng C, Shu-Wei W. 2007. Polyphenolic compound and the degree of browning in processing apple varieties. Agricutural Sciences in China 6(5), 607-612.

Zhu JK. 2002. Salt and drought stress signal transduction in plants. Annu Rev Plant Physiol Plant Molecular Biology 53, 247-273.


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