Diversity and phylogenetic analysis of pseudomonas strains isolated from Mingyong glacier, China

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Research Paper 01/10/2016
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Diversity and phylogenetic analysis of pseudomonas strains isolated from Mingyong glacier, China

Muhammad Kamran Taj, Xiuling Ji, Lianbing Lin, Qi Zhang, Yunlin Wei, Imran Taj, Zahoor Ahmed, Ajaz-Ul-Haq, Ghulam Mohammad, Sana Arif, Ashfaq Ahmed
J. Bio. Env. Sci.9( 4), 232-236, October 2016.
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On the planet genus of Pseudomonas encompasses debatably the most varied and ecologically important group of bacteria. In all of the main natural environments (Fresh-water, marine and terrestrial) members of Pseudomonas family are found in great numbers and these members also establish intimate relations with animals and plants. This universal spread and distribution represents a notable degree of genetic and physiological adaptability. Within the genus physiological traits do not limit diversity. The variety of phenotypes is also revealed at the genetic level, and evidence is increasing to advocate that the diversity of genome architecture of both the chromosomes and accessory genetic elements is of certain importance. In the present study different Pseudomonas strains were isolated from mingyong glacier. Phylogenetic analysis of 16S rRNA was done and 16S rRNA structures were applied to examine the diversity of Pseudomonas strains. Phylogenetical analysis showed that four branches are formed on the phylogenetic tree. These branches included Pseudomonas strains MY1402, MY1403, MY1408, MY1410, MY0503, MY1412, MY1416, MY1420 and MY1405 which were 98~99.9% close to 11 16S rRNA gene sequences of Pseudomonas sp (NCBI GenBank). These four groups can be easily distinguished by comparing their special characteristics in 16S rRNA secondary structures and sequences. The comparison of the 16S rRNA secondary structures of the strains showed that the Pseudomonas strains from mingyong glacier have diversity than other glaciers around the world.


Abyzov SS, Mitskevich IN, Poglazova MN, Barkov NI, Lipen Abyzov VY, Mitskevich SSIN, Poglazova MN, Barkov NI, Lipenkov VY, Bobin NE, Koudryashov BB, Pashkevich VM, Ivanov MV. 2001. Microflora of the basal strata at Antarctic ice core above the Vosto Lake. Advance in Space Research 28, 701-706.

Cladera AM, Bennasar A, Barcelo M, Lalucat J Valdes EG. 2004. Comparative Genetic Diversity of Pseudomonas stutzeri Genomovars, Clonal Structure, and Phylogeny of the Species. Journal of Bacteriology 186(16), 5239-5248.

De-Rijk P,  Wachter RD, Rna-Viz. 1997. A program for the visualisation of RNA secondary structure. Nucleic Acids Research 25(22), 4679-84.

Fantroussi S, Agathos SN. 2005. Is bioaugmentation a feasible strategy for pollutant removal and site remediation. Current Opining in Microbiology 8, 268-275.

Ginard M, Lalucat J, Tummler B, Romling U. 1997. Genome organization of Pseudomonas stutzeri and resulting taxonomic and evolutionary considerations. International Journal of Systemic Bacteriology l 47, 132-143.

Hugenholtz P, Goebel BM, Pace NR. 1998. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. Journal of Bacteriology 180, 4765-4774.

Kersters K, Ludwig W, Van-Canneyt M, De-Vos P, Gillis M, Schleifer KH. 1996. Recent changes in the classification of the pseudomonads: an overview. Systematic and Applied. Microbiology 19, 465-477.

Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJ. 2003. Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. International Journal of Systematic Evolutionary Microbiology 53 (1), 21-7.

Mathews DH, Sabina J, Zuker M, Turner DH. 1999. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. Journal of Molecular Biology 288(5), 911-40.

Miteva VI, Sheridan PP, Brenchley JE. 2004. Phylogenetic and Physiological Diversity of Microorganisms Isolated from a Deep Greenland Glacier Ice Core. Applied and Environmental Microbiology 70(1), 202-213.

Page RD. 1996. An application to display phylogenetic trees on personal computers. Computer Application in Biosciences 12(4), 357-8.

Rees HC, Grant WD, Jones BE, Heaphy S. 2004. Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods. Extremophiles 8, 63-71.

Robb FT, Place AR, Sowers HJ, Schreier S, Dassarma Fleischmann EM (Eds). 1995. Laboratory Manual, cold Spring Harbor Laborotory Press, Cold Sprinf Harbor NY.

Spiers AJ, Buckling A, Rainey PB. 2000. The causes of Pseudomonas diversity. Microbiology 146, 2345-2350.

Woese CR. 1987. Bacterial evolution. Microbiological Reviews 6, 60-69.