Int. J. Biosci.10( 1), 302-310, January 2017
The mitochondrial Cytochrome oxidase 1 (CO1) gene is used as a standardized, authenticated and reliable genetic marker for a global species-level bio-identification system. The present study was conducted to examine COI gene for Schizothorax plagiostomus, from Azad Jammu and Kashmir, to determine whether barcoding can help accurate species identification in fishes. The overall base composition of Schizothorax species were A, 25.7%; C, 28.0%; T, 28.1% and G 18.1%, A+T content 53.7% and G+C content 46.1%. The Ts/Tv bias (R) was 1.70 and 7 haplotypes were observed. The average value of haplotype diversity was Hd: 0.944±0.07 and the average value of nucleotide diversity (Pi) was 0.0032±0.001. Short fragment (721) was amplified using PCR and sequenced from nine fish samples collected from river Neelum and Jhelum, and identification of species were done in Genbank and BOLD. Two variable characters were parsimony uninformative, and 4 variable characters were parsimony informative. The overall mean intra-specific distance was 0.03±0.001 and mean inter-specific nucleotide sequence divergence was 0.404%. We emphasized that, DNA barcoding is an accurate, reliable and has the great potential for identification of freshwater fish species. The ability to assign species with high precision from DNA samples of different quality and origin has major utility in several fields, from fisheries and conservation programs to control of fish products authenticity and its sustainability.
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