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DNA based elaboration of tea genotypes cultivated in Pakistan

Sahib Gul Afridi, Habib Ahmad, Imtiaz Ahmed Khan, Mukhtar Alam

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Int. J. Biosci.9(6), 259-265, December 2016

DOI: http://dx.doi.org/10.12692/ijb/9.6.259-265


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Tea, obtained from Camellia sinensis L. is one of the most important non-alcoholic beverages of the World. Continuous selection in favor of desirable traits has reduced natural genetic diversity in tea.  Present study was conducted to estimate genetic diversity in a world collection of tea germplasm cultivated in Pakistan using Random Amplified Polymorphic DNA (RAPD) primers. A total of 42 tea accessions were characterized using 21 RAPD primers. The data obtained from PCR analysis was used for making average genetic distances matrix based on Unweighted pair group method using arithmetic averages (UPGMA) and for construction of a dendrogram as well. A high amount of genetic diversity (G.D=0-100%) was estimated among the germplasm accessions. Accessions were clustered into 2 main groups and 2 subgroups according to conventional classification of tea taxa and geographical origin of the genotypes. Tea genotypes were also separated clearly according to their main taxa (i.e. C. sinensis and C. assamica) in the world. The results obtained, will help in establishing conservation strategies for tea in Pakistan. RAPD methodology proved practical for evaluation of genetic diversity and relationship among tea genotypes.


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DNA based elaboration of tea genotypes cultivated in Pakistan

Afridi SG, Ahmad H, Alam M, Khan IA, Hassan M. 2011. DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers. African Journal of Biotechnology 10(69), 15477-15482. http://dx.doi.org/10.5897/AJB11.801

Anonymous. 2011. Economic Survey 2010-2011. Federal Bureau of Statistics, Government of Pakistan, Islamabad.

Ariyarathna C, Gunasekare K. 2006. Genetic base of tea (Camellia sinensis L.) cultivars in Sri Lanka as revealed by pedigree analysis. Journal of Applied Genetics 48(2), 125-128. http://dx.doi.org/10.1007/BF03194669

Bonner M, Rothman N, Mumford JL. 2005. Green tea consumption, genetic susceptibility, PAH-rich smoky coal, and the risk of lung cancer. Mutation Research 582, 53-60. http://dx.doi.org/10.1016/j.mrgentox.2004.12.008

Borchetia S, Das SC, Handique PJ, Das S. 2009. High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.). Scientia Horticulture 120(4), 544-550. http://dx.doi.org/10.1016/j.scienta.2008.12.007

Chen J, Wang P, Xia Y, Xu M, Pei S. 2005a. Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Genetic Resources and Crop evolution 52, 41-52. http://dx.doi.org/10.1007/s10722-005-0285-1

Chen L, Gao Q, Chen D, Xu C. 2005b. The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elitegenetic resources {Camellia sinensis (L.) O. Kuntz} preserved in a tea germplasm repository.  Biodiversity Conservation 14, 1433-1444.

Chen L, Zhou A, Yang Y.  2007. Genetic improvement and breeding of tea plant (Camellia sinensis) in China: from individual selection to hybridization and molecular breeding.  Euphytica 154, 239-248. http://dx.doi.org/10.1007/s10681-006-9292-3

Gul S, Ahmad H, Khan IA, Alam M. 2007. Assessment of genetic diversity in tea genotypes through RAPD primers. Pakistan Journal of Biological Sciences 10, 2609-2611. http://dx.doi.org/10.3923/pjbs.2007.2609.2611

Hirose M, Hoshiya T, Akagi K, Futakuchi M, Ito N. 1994.  Inhibition of mammary gland carcinogenesis by green tea catechins and other naturally occurring antioxidants in female Sprague-Dawley rats pretreated with 7, 12-dimethylbenz [alpha] anthracene. Cancer Letters 83, 149-56. http://dx.doi.org/10.1016/0304-3835(94)90312-3

Kaundun SS, Park YG. 2002. Genetic structure of  six Korean tea populations revealed by RAPDPCR markers. Crop Science 42, 594–601. http://dx.doi.org/10.2135/cropsci2002.5940

Kobayashi N, Horikoshi T, Katsuyama H, Handa T, Takayanagi K. 1998. A simple and efficient DNA extraction method for plants, especially woody plants. Plant Tissue Culture and Biotechnology 4, 76-80.

Lai JA, Yang WC, Hsiao JY. 2001. An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Botanical Bulletin- Academia Sinica 42, 9 3-100.

Liu BY, Cheng H, Li YY, Wang LY, He W, Wang PS. 2012. Fingerprinting for discriminating tea germplasm using inter-simple sequence repeat (ISSR) markers.  Pakistan Journal of Botany 44(4), 1247-1260.

Liu BY, Wang PS, Ji PZ, Xu M, Cheng H. 2008. Study on genetic diversity of peculiar sect. Thea (L.) Dye in Yunnan by ISSR markers. Journal of Yunnan Agriculture University 23(5), 302-308.

Magambo MJS, Cannell MGR. 1981. Dry matter production and partition in relation to yield of tea. Experimental Agriculture 17, 33-38. http://dx.doi.org/10.1017/S0014479700011200

Murty SG, Pate lF, Punwar BS, Pate lM, Singh AS, Fougat RS. 2013. Comparison of RAPD, ISSR, and DAMD markers for genetic diversity assessment between accessions of Jatropha curcas L. and its related species. Journal of Agriculture Science and Technology 15, 1007-1022.

Nei M, Li WH. 1979.  Mathematical model for studying genetic variation in terms of restriction endonuclease. Proceeding of National Academy of Science, USA 76(10), 5269–5273.

Paterson AH, Tanksley SD, Sorrels ME. 1991.  DNA markers in plant improvement.  Advances in Agronomy 46, 39-90.

Sayama K, Lin S, Zheng G, Oguni I. 2000.  Effects of green tea on growth, food utilization and lipid metabolism in mice. In Vivo 14(4), 481-484.

Sealy J. 1958. A revision of the genus Camellia. Royal HorticulturalSociety (Ed.), London.

Williams JGK, Kubelik ARK, Livak JJ, Rafalski A, Tingey SV. 1990.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531-6535. http://dx.doi.org/10.1093/nar/18.22.6531

Wang KR, Du YY, Shao SH, Lin C, Ye Q, Lu JL, Liang YR. 2010. Development of specific RAPD markers for identifying albino tea cultivars ‘Qiannianxue’ and ‘Xiaoxueya. African Journal of Biotechnology 9(4), 434-437. http://dx.doi.org/10.5897/AJB09.971

Yao MZ, Chen L, Wang XC, Zhao LP, Yang YJ. 2007. Genetic diversity and relationship of conal tea cultivars in china revealed by ISSR markers. Acta Agronomica Sinica 33(4), 598-604.

Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX. 1999.  POPGENE 3.2, the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada.


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