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DNA fingerprinting of millennium olive varieties in Tunisia by AFLP markers

Mnasri Rahmani Sameh, Saddoud Debbabi Olfa, Ben Saleh, Ferchichi Ali

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J. Bio. Env. Sci.4(4), 310-317, April 2014

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Abstract

Tunisia is one of the oldest agricultural settlements in history. Evidences revealed by archeological excavations indicated that olives were cultivated before about 3000 years in Tunisia. Although the importance of millennium olives, studies about molecular biodiversity and evaluation are scarce. In order to investigate intra cultivar variability on the molecular level, olive samples from nine different archeological sites were screened for amplified fragment length polymorphisms (AFLP). DNA was extracted from leaf tissue and 6 EcoRI–MseI AFLP primer combinations were used. Auto radiographs revealed 84 polymorphic markers in a total of 237 detected fragments. A set of redundant marker patterns was identified and deleted from the binary data matrix; data analysis demonstrated a high degree of polymorphism with an average of 32.7%. The analysis of AFLP profiles found in our set of olive cultivars showed a wide genetic diversity among olive germplasm. The UPGMA cluster analyses using Jaccard’s index and the Principal coordinate analysis (PCO) revealed that the genetic diversity was predominantly structured according to the morphological parameters of the fruit and the endocarp. The data obtained can be used for the varietal survey and construction of a database of millennium olive varieties in Tunisia and providing also additional information that could form the basis for the national design of breeding programs.

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DNA fingerprinting of millennium olive varieties in Tunisia by AFLP markers

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