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DNA sequence analysis, morphology and pathogenicity of Fusarium oxysporum f. sp. lentis isolates inciting lentil wilt in Pakistan

Khola Rafique, Chaudhary Abdul Rauf, Farah Naz, Ghulam Shabbir

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Int. J. Biosci.7(6), 74-91, December 2015

DOI: http://dx.doi.org/10.12692/ijb/7.6.74-91


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Fusarium wilt of lentil (Lens culinaris Medikus) incited by F. oxysporum f. sp. lentis causes huge lentil yield losses worldwide. Understanding morpho-molecular and pathogenic variation of F. oxysporum isolates is of utmost importance, which has not been previously studied in Pakistan. In this study, extensive two year (2011-12 and 2012-13) wilt surveys of seven districts viz. Chakwal, Jhelum, Gujrat, Sialkot, Layyah, Bhakkar and Khushab of Punjab, Pakistan were conducted, which revealed 100% mean disease prevalence and 25.7% mean incidence. Morphology assessment of recovered 105 isolates showed unique identification characters and variability in morphological measurements that differentiates F. oxysporum from other fusaria. Furthermore, sequencing of TEF-1α gene supported the morphological study and confirmed the associated fungi at species level. Phylogenetic analysis grouped all the selected type 31 isolates, obtained from different districts, under a single lineage within F. oxysporum species complex. Characterized isolates screened for pathogenicity revealed considerable pathogenic variability on two lentil genotypes (NARC-08-1 and Masoor-93). The inoculation of lentils confirmed the pathogenicity of 30 (96.77%) isolates on NARC-08-1 with 0 to 100% disease severity index and incidence with 19.33 to 100% yield reduction. On Masoor-93, 22 (70.97%) isolates were pathogenic with 0 to 66.66% severity index, 0 to 100% incidence and 6.47 to 53.68% yield reduction. Eight (25.81%) isolates viz. FWC15, FWJ35, FWG1, FWL6, FWL9, FWL12, FWB10 and FWK2 proved the most pathogenic resulting in highly virulent disease reaction (94.07% mean severity index and 100% mean incidence and yield reduction) and complete death of the NARC-08-1 seedlings.


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DNA sequence analysis, morphology and pathogenicity of Fusarium oxysporum f. sp. lentis isolates inciting lentil wilt in Pakistan

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