Dose dependent DNA damage in Cyprinus carpio under sub lethal concentration exposure to pesticides mixture
Paper Details
Dose dependent DNA damage in Cyprinus carpio under sub lethal concentration exposure to pesticides mixture
Abstract
Water pollution due to various pesticides is become a global problem now a days because their contents reach in the aquatic ecosystem where it poses considerable toxicological risks to non-target organisms like fish. Therefore, this study was designed to determine the in-vivo induction of DNA damage in Cyprinus carpio (common carp) under sub lethal concentration exposure to binary pesticides mixture (endosulfan – chlorpyrifos) by using single cell gel electrophoresis / comet assay. At first phase, 96-hr LC50 was determined, based on this value four sub-lethal concentrations viz. 1/3rd, 1/4th, 1/5th and 1/6th were calculated. The C. carpio exposed, separately, to aforementioned concentrations for a period of 15 days along with two control groups i.e. negative and positive. The fish peripheral erythrocytes were sampled on day 15th of exposure period from each sub lethal concentrations / treatments for the estimation of DNA damage in. The DNA damage was determined in terms of %age of damaged nuclei, genetic damage index (GDI) and cumulative tail lengths of comets (CTL). Results exhibited statistically significant (p<0.05) effects at different sub lethal concentrations in fish groups along with positive control as compared to negative control. The DNA damage was found to be concentration dependent with highest damage observed at 1/3rd LC50 exposure as compared to control groups in terms of %age of damaged nuclei, GDI and CTL of comets. The present experiment showing the potential genotoxicity of pesticides in common carp (which is non-target organism) and comet assay is useful tool to study the genotoxic effect of water-borne pollutants.
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Faiza Ambreen, Muhammad Javed, Safina Kousar, Rahila Ilyas (2017), Dose dependent DNA damage in Cyprinus carpio under sub lethal concentration exposure to pesticides mixture; IJB, V11, N3, September, P171-177
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