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Drug resistance of Staphylococcus aureus in sinusitis patients

Research Paper | June 1, 2011

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Sasikala Shanmugam, Ramganesh Selvarajan, Sundararaj Thangiah

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Int. J. Biosci.1( 3), 63-71, June 2011


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In this study on Sinusitis patients, we obtained 45 strains of Staphylococcus aureus. Antibiotic pattern of Staphyloccus aureus showed that resistance to Quinolones was 21% and 33% towards ciprofloxacin andoflaxacin respectively. Resistance to cephalosporins was 50% to cefuroxime, 41% and 50% to cefaperazone and cefotaxime respectively. Least resistance was noticed against aminoglycosides viz. Amikacin 47% and Gentamicin 21%. Resistance to Ampicillin and amoxicillin was 60% and 64% respectively. Oxacillin resistance was seen in 26% of the strains. Of the 45 isolates, 6 were found to be resistant for oxacillin . All these six isolates were subjected to Polymerase Chain Reaction (PCR) and they possessed the mecA gene. Correlation existed between the presence of mecA gene and oxacillin resistance in Staphylococcus aureus and these strains can be considered as MRSA and the patients can be advised for vancomycin therapy. Oxacillin resistance determination by phenotypic methods takes 24 hours to infer whereas PCR for mecA gene took only 6 hours. So the PCR techniques for the detection of mecA gene can be considered as gold standard (Rapid, Quick and accurate diagnosis) method for the detection of MRSA in spite of the cost involved.


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Drug resistance of Staphylococcus aureus in sinusitis patients

Araj, Talhouk RS, Simaan CJ, Maasad. 1991. Discrepancies between mecA gene PCR & Conventional tests used for detection of Methicillin resistant Staphylococcus aureus. International Journal of Antimicrobial Agent 11, 45-52.

Archer GL, Pennell E. 1990. Detection of methicilin resistance in Staphylococcus by using a DNA probe. Antimicrobial Agents and chemotherapy 34, 1720-4.

Bect WD, Berger B, Kayser FH. 1980. Additional DNA in methicillin resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. Bacteriol. 165, 373 – 378

Bogle, Bob RB, Graciela S. 1999. Acquiring vancomycin resistant Staphylococcus aureus perspective on measures needed for control. Ann. Intern. Med. 124(3), 329-34.

Boyce JM. 1990. Increasing prevalence of Methicillin Resistant Staphylococcus aureus in the United states. Infect control Hosp, Epidemiol. 11, 639-42

Nord. CE. 1995. The role of anaerobic bacteria in recurrent episodes of sinusitis and tonsillitis ClisInfect. Dis.20, 1512-1524.

Chikramane SG, Mathews N, Stewart D. 1991. Tn 54 inserts in methicillin-resistant Staphylococcus aureus from Australia and England. J. Gen. Microbial. 137, 1303-1311.

Clewell DB. 1998. Conjucative transposons  p.130-139. In F.j.de Bnijn, J.R. Lupski and G.M. Weinstock (ed.), Bacterial Genomes, Chapman and Hall, New York, N.Y.

Lews S, Montgomery. 1983.Sphenoid Sinusitis. N. Eng. J. Med. 309, 1149-1154.

Doem GV, Bruggeman AB, Pierce G, Holley HP, Rauch A. 1997. Antibiotic resistance among clinical isolates of Haemophilus influenzae in the United states in 1994 and 1995 and detection of beta – lactamase positive strains resistant to amoxicillin-clavanulate; results of a national mulicenter surveillance study Antimicrob. Agents Chemother. 35, 1661-1665.

Dubin M, Childeramance S. 1991. Physical mapping of mecA gene region of on American methicillin resistance Staphylococcus aureus strains. Antimicrobial Agents Chemother. 35, 1661-1665.

Duong G, Thompson. 2004. Methicillin resistance Staphylococcus aureus Antimicrob. Agents Chemother. 37, 1552-57.

Finegold S.M. 1997. Anaerobic bacteria in human disease .Orland’s, FL Academic press Inc.

Gold SM, Tami TA. 1997. Role of middle meatus aspiration culture in the diagnosis of Chronic Sinusitis. Laryyngoscope 107, 1586-1589.

Gordts F, AbuNassar I, Clement PA, Pieard D, Kaufman KL. 1999. Bacteriology of the middle meatus in children. Int. J. Pediatr. Otorhinolaryngol 48, 163-7.

Gwaltney J.J. 1996. Acute community acquired sinusitis .Clinical infection of disease 23,1209-1212.

Hart SA, Phelps K, Mulligan M.E, 1995. Instability and discriminatory power of methicillin resistant Staphylococcus aureus typing by restriction endonucleases analysis of plasmid DNA compared with those of other molecular methods. J. Cl. Microbial. 33, 2022-6.

Hiramatsu K, Hanaki H, Yubuta, Oguri T, Tenover FC. 1997. Methicillin- Resistant Staphylococcus aureus clinical strains with reduced vancomycin susceptibility. J. Antimicrobial Chemother. 40, 135-6.

Incaudo W. 1998. Diagnosis and treatment of acute and subacute sinusitis in children and adults. Clin. Rev. Allergy Immunol. 16, 157-204.

Jacobs MR, Bajaksouzian Z, Pankuch A. 1999. Susceptibilities of Streptococcus pneumoniae and Haemophilus influenzae to 10 oral Antimicrob agents based on pharmacodynamic parameters, US surveillance study, Antimicrob. Agents Chemother. 460-465.

Kazuhisa M, Wakio M. 1991. Identification of Methicillin-Resistant Strains of Staphylococci by Polymerase Chain Reaction. p. 2240-2244

Lacey RW. 1972. Genetic control in Methicillin Resistant strains of Staphylococcus aureus. J. Med. Microbial. 5, 197-508.

Lee CA. 1996. Pathogenicity islands and the evolution of bacterial pathogens. Infect. Agents Dis. 5, 1-7.

Mark AR, Raynor R, Edward JF, Hajo G. 2002. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). PNAS 99 (11), 7687-7692.

Nadel L, Kennedy. 1999. Endoscopically guided sinus cultures in normal subjects. J. Rhinol. 13, 37-90.

Orobello PR, Belcher. 1991. Microbiology of chronic Sinusitis in children. Arch. Otolaryngol. Surg. 117, 980-983.

Paterson, David L. 2001. Reduced susceptibility of Staphylococcus aureus to vancomycin – A review of current knowledge. Communicate Diseases intelligence 23.

Quintiliani R, Courvalin. 1995. Mechanism of resistance to antimicrobial agents. In P.R.Murray, E.J.Barom. M.A. Pfaller, F.C.Tenover, and R.H.Yolden (ed.,), Manual of clinical Microbiology. American society for Microbiology, Washington.

Rechia GD, Hall. 1995. Gene cassettes; a new class of mobile element. Microbiology 141, 3015-3027

Salord F, Gaussorgues, Marti-Flich J. 1990. Noscomial Maxillary sinusitis during mechanical ventilation- a prospective comparison of orotracheal versus the nosotracheal route for incubation. Intensive Care Med. 18, 390-393.

Sjostrom JE, Lofdahi S, Philipson. 1975. Transformation reveals a chromosomal locus of the genes for the methicillin resistance of Staphylococcus aureus. J. Bacteriol. 123, 905-913.

Spector SL, Bemstein IL, Berger LIJT, Kaliner MA, Schuller DE. 1998. Parameters for the diagnosis and management of sinusitis- complete guidelines and references. J. Allergy Clin. Immunol. 102, 117-44.

Tos M. 1983. Distribution of Mucous Producing elements in the respiratory tract. Differences between upper and lower ainway. Eur J. Respir Dis. Suppl. 128, 296-79.

Unals, Wernerk, Degirolami P, Barsantif, Ellopoyles G. 1994. Comparison of tests for the detection of methicillin –resistant Staphylococcus aureus in a clinical microbiology laboratory. Antimicrobial Agents Chemother. 38, 345-7.

Vogan, Bolger WE, Keyes. 2000. As endoscopically guided Sinonal Cultures: a direct comparison with maxillary sinus aspirates cultures. Otolaryngol Head Neck Surg. 12, 370-373.

Walder, Mill GJ, Brown AD, Ledema I, Salomon N, Bluestone CD. 1981. Acute maxillary sinusitis in children. N. Engi J. Med. 304, 749-54.

Yauck JS, Smith TL. 2006. Patient undergoing sinus surgery constructing a demographic Profile. 116, 135-91.