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Eco-toxicity of Neonicotinoid: a case study on the impact of imidacloprid using Drawida willsi earthworm as bioindicator

Research Paper | May 1, 2013

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Anindita Bhattacharya, Sanjat Kumar Sahu

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Int. J. Biosci.3( 5), 78-86, May 2013

DOI: http://dx.doi.org/10.12692/ijb/3.5.78-86


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Many pesticides are used in the world and in India to boost the crop productivity, among which imidacloprid; a neonicotinoid is widely used now a day due to its low toxicity. These pesticides no doubt have protected the crops from the dangerous pest but in turn are becoming dangerous for soil health. Therefore an experiment was conducted to find out the eco-toxicity of imidacloprid. For this, earthworm (Drawida willsi, Michaelsen) and soil was collected from such agricultural field where there had no record of input of agrochemicals. Different concentrations of imidacloprid were prepared in dilution with acetone and sprayed on the soil surface. Five replicates for each concentration of the pesticides were prepared. Then ten numbers of juvenile, immature and adult earthworms were added separately into all the replicates of different concentrated samples. All the samples were kept in the laboratory under close vigil for 96 hours. Number of earthworm death with respect to doses and replicates were recorded and the 96h LC50 values for juvenile, immature and adult earthworm were calculated by Finney’s Probit Method (Finney, 1971). It was found that the 96 h LC50 values with their 95% confidence limit of juvenile, immature and adult earthworm were 4.43, 7.96 and 12.45 mg a.i. imidacloprid/kg dry soil respectively. Although the recommended dose of imidacloprid was lower than the 96 h LC50 values of D. willsi for imidacloprid, but still it could affect the soil biota by altering its vital rates and metabolism due to bioaccumulation of the agrochemicals.


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Eco-toxicity of Neonicotinoid: a case study on the impact of imidacloprid using Drawida willsi earthworm as bioindicator

Booth LH O, Halloran K. 2001. A comparison of biomarker responses in the earthworm Aporrectodea caliginosa to the organophosphorus insecticides diazinon and chlropyrifos. Environmental Toxicology and Chemistry 20(11) 2494-2502. http://dx.doi.org/10.1002/etc.5620201115

Booth  LH,  Heppelthwaite  VJ,  Eason  CT. 1998. Choline esterase and glutathione s-transfer in the earthworm Aporrectodea caliginosa as biomarkers of organophosphate exposure. Proceedings of the 51st New Zealand Plant Protection Conference 138-142.

Capowiez Y, Dittbrenner N, Rault M,Triebskorn R, Hedde M, Mazzia C. 2010. Earthworm cast production as a new behavioural biomarker for toxicity testing. Environmental Pollution 158, 388–393. http://dx.doi.org/10.1016/j.envpol.2009.09.003

Capowiez Y, Bastardie F, Costagliolia G. 2006. Sublethal effects of Imidacloprid on burrowing behaviour of two earthworm species L: Modification of the 3D burrow systems in artificial cores and consequences on gas diffusion in soil. Soil Biology and Biochemistry 38(2), 285-293. http://dx.doi.org/10.1016/j.soilbio.2005.05.014

Capowiez Y, Bérard A. 2006. Assessment of the effects of imidacloprid on the behavior of two earthworm species (Aporrectodea nocturna and Allolobophora icterica) using 2 Dterrraria. Ecotoxicology and Environmental Safety 64, 198–206. http://dx.doi.org/10.1016/j.ecoenv.2005.02.013

Capowiez Y, Rault M, Costagliolia G, Mazzia, C. 2005. Lethal and sublethal effects of imidacloprid on two earthworm species (Aporrectodea nocturna and Allolobophera icterica). Biology and Fertility of Soils 41(3), 135-143. http://dx.doi.org/10.1007/s00374-004-0829-0

Capowiez Y, Pierret A, Moran CJ. 2003a. Characterisation of the threedimensional structure of earthworm burrow systems using image analysis and mathematical morphology. Biology Fertility of Soils 38, 301–310. http://dx.doi.org/10.1007/s00374-003-0647-9

Capowiez Y, Rault M, Mazzia C, Belzunces, L. 2003b. Earthworm behaviour as a biomarker – a case study using imidacloprid. Pedobiologia 47, 542–547. http://dx.doi.org/10.1078/0031-4056-00226

Curry JP, Doherty P, Purvis G, Schmidt O. 2008. Relationship between earthworm populations and management intensity in cattle-grazed pastures in Ireland. Applied Soil Ecology 39(1), 58-64. http://dx.doi.org/10.1016/j.apsoil.2007.11.005

Edwards CA, Lofty JR. 1982. Nitrogenous fertilizers and earthworm populations in agricultural soils. Soil Biology and Biochemistry, 14(5), 515-521. http://dx.doi.org/10.1016/0038-0717(82)90112-2

Faheem M, Khan MF. 2010. Toxicity of imidacloprid (Nicotinoid) against earthworm, Pheretima posthuma with special reference to its effects on protein. Journal of Basic and Applied Sciences, 6(1), 55-62.

Finney   DJ. 1971. Probit analysis, Cambridge Uni. Press, Cambridge.

Hund-Rinke K, Wiechering H. 2001. Earthworm avoidance test for soil assessments : An alternative for acute and reproduction tests. Journal of Soils and Sediments 1, 15-20. http://dx.doi.org/10.1007/BF02986464

Hund-Rinke K, Achazi R, Römbke J, Warnecke D. 2003. Avoidance test with Eisenia fetida as indicators for the habitat function of soils: Results of a laboratory comparison test. Journal of Soils and Sediments 3(1), 7-12. http://dx.doi.org/10.1007/BF02989462

Leland JE, Mullis DE, Berry DF, 2001. Evaluating environmental hazards of land applying composted diazinon using earthworm bioassay. Journal of Environmental Science and Health 36(6), 821-834. http://dx.doi.org/10.1081/PFC-100107415

Lukkari T, Aatsinki M, Vaisanen A, Haimi J. 2005. Toxicity of copper and zinc assessed with three different earthworm tests. Applied. Soil Ecology 30(2), 133-146. http://dx.doi.org/10.1016/j.apsoil.2005.02.001

Mahajan  S,  Kanwar  SS,  Sharma  SP.  2007. Long-term effect of mineral fertilizers and amendements on microbial dynamics in an alfisol of western Himalayas. Indian Journal of Microbiology, 47(1), 86-89. http://dx.doi.org/10.1007/s12088-007-0016-8

Morowati M. 2000. Histochemical and histopathological study of the intestine of the earthworm (Pheretima elongate) exposed to a field dose of the herbicide glyphosate. The Environmentalist 20(2), 105-111. http://dx.doi.org/10.1023/A:1006704009184

Mostert MA, Schoeman AtS, Merwe Mac, van der. 2002. The relative toxicities of insecticides to earthworm of Pheretima (Oligochaeta). Pest Management Science 58(5), 446-450. http://dx.doi.org/10.1002/ps.473

Mostert MA, Schoeman AS, Van der Merwe, M. 2000. The toxicity of five insecticides to earthworms of the Pheretima group, using an artificial soil test. Pest Management Science 58, 1093–1097. http://dx.doi.org/10.1002/1526-4998(200012)56:12<1093::AID-PS259>3.0.CO;2-6

Mullins JW, 1993. Imidacloprid. A new nitroguanidine insecticide. (In: Pest control with enhanced environmental safety). ACS Symposium Series, American Chemical Society 524, 183-198.

Muthukaruppan G, Ganasekaran P. 2010. Effect of Butachlor herbicide on earthworm Eisenia fetida – its histological perspicuity. Applied and Environmental Soil Science 2010, 1-5. http://dx.doi.org/10.1155/2010/850758

Muthukaruppan G, Janardhanan S. Vijayalakshmi G. 2005. Sublethal toxicity of the herbicide butachlor on the earthworm Perionyx sansibaricus and its histogical changes (5pp). Journal of Soils and Sediments 5(2), 82-86. http://dx.doi.org/10.1065/jss2004.09.111

Neuhauser EF,  Callahan CA. 1990. Growth and reproduction of the earthworm Eisenia fetida exposed to sub-lethal concentrations of organic chemicals. Soil Biology and Biochemistry 22(2),175-179. http://dx.doi.org/10.1016/0038-0717(90)90083-C

Panda S, Sahu SK. 1999. Effects of malathion on the growth and reproduction of Drawida willsi (Oligochaeta) under laboratory conditions. Soil Biology and Biochemistry 31, 363-366. http://dx.doi.org/10.1016/S0038-0717(98)00135-7

Paoletti MG. 1999.  The  role  of  earthworms  for assessment  of  sustainability  and  as  bioindicators. Agriculture Ecosystems and Environment 74(1-3), 137-155. http://dx.doi.org/10.1016/S0167-8809(99)00034-1

Rallmbke J, Jaonsch S, Junker T, Pohl B, Scheffezyk A, Schallnaay HJ. 2007. The effect of tributyltin-oxide on earthworm, springtails and plants in artificial and natural soils. Archives of Environmental Contamination and Toxicology, 52(4), 525-534.http://dx.doi.org/10.1007/s00244-006-0099-y

Reddy MV, Goud AN, 1987. Impact of inorganic fertilizers on the earthworm population density of wetland rice (Oryza sativa) agroecosystems. Proceedings of the National Symposium on Ecotoxicology 154-158.

Ribera D, Narbonne JF, Arnaud C, Saint-Denis M. 2001. Biochemical responses of the earthworm Eisenia fetida andrei exposed to contaminated artificial soil, effect of carbaryl. Soil Biology and Biochemistry 33(7-8), 1123-1130. http://dx.doi.org/10.1016/S0038-0717(01)00035-9

Sardo AM, Soares AM VM. 2010. Assessment of the effects of the pesticide imidacloprid on the behavior of the aquatic oligochaeta Lumbricus variegatus. Archives of Environmental Contamination and Toxicology 58(3), 648-656. http://dx.doi.org/10.1007/s00244-010-9470-0

Senapati BK, Dash MC. 1984. Functional role of earthworms in decomposer subsystem. Tropical Ecology 25(2), 54-73.

Suchail S, Guez D. Belzunces LP. 2001. Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis melifera. Environmental Toxicology and Chemistry 20(11), 2482-2486. http://dx.doi.org/10.1002/etc.5620201113

Syers JK, Springett JA, 1984. Earthworms and soil fertility. Plant and Soil 76 (1-3), 93-104. http://dx.doi.org/10.1007/BF02205570

Tiwari SC. 1993. Effects of organic manure and NPK fertilization on earthworm activity in an Oxisol. Biology and Fertility of Soils 16(4), 293-295. http://dx.doi.org/10.1007/BF00369307

Torres JB, Ruberson JR. 2004. Toxicity of thiamethoxam and imidacloprid to Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) nymphs associated to aphid and whitefly control in cotton. Neotropical Entomology 33(1), 99-106. http://dx.doi.org/10.1590/S1519-566X2004000100017

Viswanathan, R. 1997. Physiological basis in assessment of Ecotoxicity of pesticides to soil organisms. Chemosphere, 35(1-2), 323-334. http://dx.doi.org/10.1016/S0045-6535(97)00158-6

Zang Y, Zhong Y, Luo Y, Kong ZM. 2000. Genotoxicity of two novel pesticides for the earthworm Eisenia fetida. Environmental Pollution 108(2), 271-278. http://dx.doi.org/10.1016/S0269-7491(99)00191-8