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Economical and quick indexing of citrus greening disease in Sargodha, Pakistan

Research Paper | January 1, 2021

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Ashara Sajid, Muhammad Usman Ghazanfar, Zahoor Hussain, Salman Ahmed, Yasir Iftikhar

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Int. J. Biosci.18( 1), 228-232, January 2021

DOI: http://dx.doi.org/10.12692/ijb/18.1.228-232


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Citrus greening disease (CGD), a century old prokaryotic disease is one of the most destructive and widely distributed diseases in the citrus growing areas of the world. This disease has become a serious threat to citrus industry. Zinc spray gives a clue for the disease as persistence of symptoms even after the foliar application. The early detection of CGD is one of the key steps to manage the disease in time. Therefore, during a survey in 2018-2019, the study was carried out for routine, economical and quick detection of CGD in infected samples collected on the basis of symptomology. The samples were collected from the citrus orchards infected with greening disease. Iodo-starch test was performed to observe the accumulation of starch in the symptomatic leaves. Moreover, the infected bud-wood were grafted on healthy plants showed the mottling symptoms after 6-8 months. Dark grey edges of the infected leaves indicated the accumulation of starch. Among the five varieties as indicator, Sweet orange, kinnow and Feutralls’ early showed the mottling symptoms on indicator citrus plants. This confirmed the transmissibility of CGD and iodo-starch test was found reliable along with symptomology and vegetative propagation for early and quick detection of CGD. The iodo-starch test was effective as backed up by vegetative transmission.


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Economical and quick indexing of citrus greening disease in Sargodha, Pakistan

Akhtar MA, Ahmad I. 1999. Incidence of citrus greening disease in Pakistan. Pakistan Journal of Phytopathology 11, 1-5.

Batool A, Iftikhar Y, Mughal SM, Khanmm, Jaskani MJ, Abbas M, Khan IA. 2007. Citrus greening disease-a major cause of citrus decline in the world-a review. Hort. Sci.(Prague) 34(4), 159-66.

Bové JM, Chau NM, Trung HM, Bourdeaut J, Garnier M. 1996. Huanglongbing (greening) in Vietnam: detection of Liberobacter asiaticum by DNA-hybridization with probe in 2.6 and PCR-amplification of 16S ribosomal DNA. In: Proceedings of 13th International Organization of Citrus Virologists Conference 13, 258-266.

Bové JM. 2006. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. Journal of plant pathology 88(1), 7-37.

Chohan SN, Qamar R, Sadiq I, Azam M, Holford P, Beattie A. 2007. Molecular evidence for the presence of huanglongbing in Pakistan. Australasian Plant Disease Notes 2, 37-8.

Cochran LC. 1976. The occurrence of greening disease in Pakistan. In: Proceedings of 7th International Organization of Citrus Virologists Conference 7, 21.

Etxeberria E, Gonzalez P, Dawson W, Spann T. 2007. An iodine-based starch test to assist in selecting leaves for HLB testing. Document HS1122. Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Gainesville. http://edis.ifas.ufl.edu

Graca JD. 1991. Citrus greening disease. Annual review of phytopathology. 29, 109-36.

Iftikhar Y, Rauf S, Shahzad U, Zahid MA. 2016. Huanglongbing: Pathogen detection system for integrated disease management–A review. Journal of the Saudi Society of Agricultural Sciences 15(1), 1-11.

Lopes SA, Frare GF. 2008. Graft transmission and cultivar reaction of citrus to ‘Candidatus Liberibacter americanus’. Plant Disease 92(1), 21-24.

Polek M, Vidalakis G, Godfrey K. 2007. Citrus Bacterial Canker Disease and Huanglongbing (Citrus Greening). 10.3733/ucanr.8218. https://escholarship. org/uc/item/48v1v164

Razi MF, Khana IA, Jaskani MJ, Basra SM. 2012. Graft transmission and biological indexing of Huanglongbing on local Citrus germplasm. Science International (Lahore). 24(2), 153-158.

Rehman MA, Iqbal Z, Ahmad S, Asim M, Rehman A, Raza M, Raza W, Anjum M, Ahmad G. 2020. Screening of citrus cultivars against root-knot nematode (Tylenchulus semipenetrans). International Journal of Biosciences. 17(1), 57-62.

Saifullah, Khan IU, Iftikhar Y, Khan, SA, Jaskani MJ, Samiullah, Tariq RM. 2015. Quick indexing of Huanglongbing on the basis of symptomology and iodo-starch test in relation to environmental factors. Pakistan Journal of Agricultural Sciences 52(4), 1005-1009.