Effect of arbuscular mycorrhiza fungi on the growth, nutrient uptake, root infectivity and soil colonisation of rough lemon (Citrus jambhiri) seedlings

Paper Details

Research Paper 01/02/2021
Views (333) Download (485)

Effect of arbuscular mycorrhiza fungi on the growth, nutrient uptake, root infectivity and soil colonisation of rough lemon (Citrus jambhiri) seedlings

DK Chebet, FK Wanzala, LS Wamocho
Int. J. Agron. Agri. Res.18( 2), 25-31, February 2021.
Certificate: IJAAR 2021 [Generate Certificate]


The effect of Arbuscular mycorrhiza (AM) fungi on growth, nutrient uptake and root infectivity was determined in Rough Lemon (Citrus jambhiri) seedlings raised under four phosphorus regimes in sand culture and also in sand/nitrosol sterile and unsterile conditions. Inoculation with AM fungi increased the plant height, leaf number and stem girth in relation to un-inoculated seedlings grown under equivalent P concentrations. An increase in plant height, leaf number and stem girth also occurred in both inoculated sterile and un-sterile sand/nitrosol media in relation to un-inoculated sterile and unsterile media. Arbuscular mycorrhiza also increased the leaf area and the root, leaf and stem fresh and dry weights and also caused an increase in the uptake of phosphorus and potassium in the leaf tissues. It also favoured mycorrhizal infectivity of roots and increased the root absorptive surface area. This study indicates that AM fungi improves the capacity of tropical fruit to absorb and utilize plant nutrients possibly by increasing the effective root surface area from which available form of nutrients are absorbed and also by increasing access of roots by bridging the depletion zones. Inoculating seedlings with arbuscular mycorrhizal fungi helps to alleviate the adverse effects of global warming and climate change. As a low cost technology, arbuscular mycorrhizal inoculation is recommended as part of the regular practise for incorporating into nursery media used for tropical fruit seedling propagation.


Al-Karaki GN. 2013. The effect of arbuscular mycorrhizal fungi on the establishment of sour orange (Citrus aurantium) under different levels of phosphorus. VII. International symposium on mineral nutrition of fruit crops book series. Acta Horticulturae 984, 103-108.

Chebet D, Kariuki W, Wamocho L, Rimberia F. 2020. Effect of Arbuscular mycorrhizal inoculation on growth, biochemical characteristics and nutrient uptake of passion fruit seedlings under flooding stress. International Journal of Agronomy and Agricultural Research (IJAAR) 16(4), 24-31.

Chebet DK. 2020. Effect of Arbuscular Mycorrhizal inoculation on the growth and performance of tropical fruit seedlings under saline, flooding and nutrient stress. PhD Thesis, Jomo Kenyatta University of Agriculture and Technology, Kenya pages 55-119.

Cruz AF, Ishii T, Kadoya K. 2000. Effect of arbuscular mycorrhizal fungi on tree growth, leaf water potential and levels of 1-aminocyclopropane-1-carboxylic acid and ethylene in the roots of papaya under water stress conditions. Mycorrhiza 10, 121-123.

Elsen A, Baimey H, Swennen R, De Waele D. 2003. Relative mycorrhizal dependency and mycorrhiza-nematode interaction in banana cultivars differing in nematode susceptibility. Plant and Soil 256, 303-313.

Estrada BE, Aroca R, Barea JM, Ruiz-Lozano JM. 2013. Native arbuscular mycorrhizal fungi isolated from a saline habitat improved maize antioxidant systems and plant tolerance to salinity. Plant Science 201, 43-51.

Fidelibus MW, Martin CA, Stutz JC. 2001. Geographic isolates of Glomus increase root growth and whole-plant transpiration of Citrus seedlings grown with high phosphorus. Mycorrhiza 10, 231-236.

Khade SW, Rodrigues BF. 2009. Studies on Effects of Arbuscular Mycorrhizal (Am.) Fungi on Mineral Nutrition of Carica papaya L. Notingham Botanicaland Horticulture Agrobot. Cluj 37(1), 183-186.

Mason PA, Wilson J. 1994. Harnessing symbiotic associations: vesicular-arbuscular mycorrhizas, p 165-175 In: Leakey, R.R.B., Newton, A.C. (Eds.), Tropical trees: potential for domestication and the rebuilding of forest resources. HMSO, London.

Michelson A. 1992. Mycorrhiza and root nodulation in tree seedlings from five nurseries in Ethiopia and Somalia. For. Ecol. Manage 48, 335-344.

Millner PD, Kitt DG. 1992. The Beltsville method for soilless production of vesicular-arbuscular mycorrhizal fungi. Mycorrhiza 2, 9-15.

Muok OB, Ishii T. 2006. Effect of arbuscular mycorrhizal fungi on tree growth and nutrient uptake of Sclerocarya birrea under water stress, salt stress and flooding J. Jap. Soc. Hortic. Sci 75, 26-31.

Rutto LK, Mizutani F, Asano Y, Kadoya K. 2002a. Effect of inoculation with arbuscular mycorrhizal (AM) fungus on phosphorus nutrition in loquat seedlings. Bull. Exp. Farm Fac. Agr., Ehime Univ 24, 1-7.

Rutto LK, Mizutani F, Asano Y, Kadoya K. 2002b. Effect of root-zone flooding on mycorrhizal and non-mycorrhizal peach seedlings. Scientia horticulturae 94, 285-295

Rydlová J, Püschel D, Sudová R, Gryndler M, Mikanová O. Vosátka M. 2011. Interaction of arbuscular mycorrhizal fungi and rhizobia: Effects on flax yield in spoil-bank clay. Journal of Plant Nutrition and Soil Science 174, 128-134.

Schnepf A, Leitner D, Klepsch S, Pellerin S, Mollier A. 2011. Modelling phosphorus dynamics in the soil-plant system. In Bünemann EK, Obserson A, Frossard E, eds, Phosphorus in Action: Biological Processes in Soil Phosphorus Cycling pp 113-133 Heidelberg: Springer.

Sieverding E. 1991. Vesicular-arbuscular mycorrhiza management in tropical ecosystems. GTZ. Eschborn, p 371.

Smith SE, Smith FA. 2011. Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystems scales. Annual Review of Plant Biology 63, 227-250.

Sundar SK, Palavesam A, Parthipan B. 2010. Effect of native dominant AM fungus and PGPRs on growth and biochemical characteristics of medicinally important Indigofera aspalathoides Vahl. ex. DC. International Journal Biology and Biotechnology 7, 59-67.

Suri VK, Choudhary AK. 2013. Effects of vesicular arbuscular mycorrhizae and applied phosphorus through targeted yield precision model on root morphology, productivity, and nutrient dynamics in soybean in an acid alfisol. Comm Soil Science Plant Analysis 17, 2587-2604.

Tas B. 2014. Effect of the Mycorrhiza Application on the Agronomical Properties of Sweet Corn Varieties. Journal of Agriculture and Allied Sciences 3(2), 41.47.

Vaseghmanesh T, Kordlaghari KP, Neia M, Kelidari A. 2014. The response of yield components of sunflower to mycorrhiza inoculation and phosphorus fertilizer Annals of Biological Research 4(3), 101-104.

Wamocho LS. 1998. Studies on the use of vesicular arbuscular mycorrhizal fungi for fruit production in Kenya. PhD Thesis, Jomo Kenyatta University of Agriculture and Technology.

Yano-Melo AM, Saggin Jr OJ, Maia LC, Melo NL. 1999. Effects of arbuscular mycorrhiza fungi on the acclimatization of micropropagated banana plantlets. Mycorrhiza 9, 119-123.

Yaseen T, Burni T, Hussain F. 2012. Effect of Arbuscular Mycorrhizal inoculation on nutrient uptake, growth and Productivity of chickpea (Cicer arietinum) varieties. International Journal of Agronomy and Plant Production 3(9), 334.345.