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Research Paper | December 7, 2022

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Effect of arbuscular mycorrhizal fungi inoculation on the antioxidant property and volatile organic compounds emission by Cymbopogon citratus L.

Veena Adishesha, KM. Srinivasa Murthy

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Int. J. Biosci.21(6), 286-297, December 2022

DOI: http://dx.doi.org/10.12692/ijb/21.6.286-297

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Abstract

Arbuscular mycorrhizal (AM) fungi influence host plants in the synthesis of many secondary metabolites with pharmaceutical importance. The effect of AM fungi on metabolite production in the Cymbopogon citratus is less studied. The present study investigates the changes influenced by the two AM fungi Glomus mosseae and Glomus fasciculatum on the chemical composition of C. citratus. The bioactive compounds produced in treated and control plants were tested for antioxidant activity and further characterized by Gas Chromatography-Mass Spectrometry, High-Performance Liquid Chromatography techniques. It was found that AM fungal inoculation in mixed culture conditions showed increased radical scavenging activity with minimum IC50 value of 19.08µg/mL and compound production at 90 days of treatment. In the treated plant extract the metabolites were present at a higher concentration as compared with control plants. The GC-MS results are supported by the HPLC spectrum which showed a similar outcome. This study proves that inoculation of AM fungi in combination is more beneficial in influencing the production of bioactive compounds than the pure culture.

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Effect of arbuscular mycorrhizal fungi inoculation on the antioxidant property and volatile organic compounds emission by Cymbopogon citratus L.

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