Effect of bacterial endophytes isolated from citrus on the physiology of Brassica Oleracea

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Research Paper 01/06/2018
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Effect of bacterial endophytes isolated from citrus on the physiology of Brassica Oleracea

Sehrish Mushtaq, Muhammad Shafiq, Muhammad Asim, Muhammad Saleem Haider
Int. J. Biosci.12( 6), 225-234, June 2018.
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Abstract

Endophytes have a symbiotic relationship with the different parts of plants and could play a very important role in supporting the plant growth. The effects of endophytic bacterial communities in cabbage have not been studied yet in Pakistan. In present study, ten different genera of bacterial endophytes isolated from citrus leaves were selected to estimate their effects on cabbage physiology at seedlings stage in glass house. To know the extent of colonization of these bacterial endophytes on cabbage as a host or non-host tissues bacterial suspension (108 CFU mL-1) were inoculated on the backside of the cabbage seedlings leave by injection syringe in green house. Five weeks after inoculation the plants were analyzed for the physical (SL, RL, SFW, RFW, SDW, RDW), bio-physical (Relative leaf water contents) & physiological (Phenolic, Flavonoids, Total soluble sugars, chlorophyll a, b& carotenoid contents) by using standard methods describe in text. According to results Bacillus safensis (shoot length, chlorophyll b contents), Bacillus megaterium (root length, phenolic compound), Pseudomonas aeruginosa (root fresh weight, total soluble sugars, flavonoids and carotenoids contents), Staphylococcus haemolyticus (shoot fresh/dry weight and relative leaf water contents)imparts beneficial effects on physiological functioning of cabbage in comparison to non-inoculated plants. Results indicated that test microbial endophytes possessing a dynamic role to improve plant growth and could be used as inoculants to establish a sustainable crop production system. However, a comprehensive approach is needed to evaluate the potential of these bacterial endophytes to improve the quality and yield in cabbage under field conditions.

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