Effect of beta-aminobutyric acid (BABA) on enzymatic and non-enzymatic antioxidants of Brassica napus L.under drought stress

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Research Paper 01/11/2013
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Effect of beta-aminobutyric acid (BABA) on enzymatic and non-enzymatic antioxidants of Brassica napus L.under drought stress

Peyman Rajaei, Neda mohamadi
Int. J. Biosci.3( 11), 41-47, November 2013.
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Water stress is one of the most important environmental factors that affect plant growth and development, and limit plant production. Many regions in Iran suffer from water deficit. To overcome these limitations for improving crop yield, it is important to increase stress tolerance of crops, such as Brassica napus L. The broad spectrum protective effect of the non-protein amino acid β-aminobutyric acid (BABA) against numerous plant stresses has been well-documented in the literature. This research shows a possibility to increase plant tolerance for drought stress through effective priming of the preexisting defense pathways in rapeseed plant. Pretreatment of plants with BABA increased ascorbate, anthocyanin and flavonoid content, while decreased DHA content in water stress plants. Calcium content was significantly increased in BABA treated plants. In drought stress condition, activity of SOD, APX and POD were elevated over the controls, while CAT activity decreased. In plants which pretreated with BABA and then exposed to drought stress the activity of mentioned enzymes increased.


Abdul  Jaleel  C,  Manivannan  P,  Sankar  B, Kishorekumar A, Gopi R, Somasundaram R, Panneerselvam R. 2007. Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potentials and secondary metabolite accumulation. Colloids and Surfaces B, Biointerfaces 60, 201–206.

Allen GJ, Chu SP, Schumacher K, Shimazaki CT, Vafeados D, Kemper A, Hawke SD, Tallman G, Tsien RY, Harper JF, Chory J, Schroeder JI. 2000. Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant. Science 289, 2338-2342.

Asada K. 1999. The water-water cycle in chloroplasts, scavenging of active oxygens and dissipation of excess photons. Ann. Rev. Plant Physiol 50, 601-639. http://dx.doi.org/10.1126/science.289.5488.2338

Baisak R, Rana D, Acharya PBB, Kar M. 1994. Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress.Plant Cell Physiol 35, 489-495.

Bian S, Jiang Y. 2009. Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Scientia Horticulturae 120, 264-270.

Brevedan RE, Egli DB. 2003. Short periods of water stress during seed filling, leaf senescence and yield of soybean. Crop Science 43, 2083-2088. http://dx.doi.org/10.2135/cropsci2003.2083

Cabuslay GS, Ito O, Alejar AA. 2002. Physiological evaluation of rice (Oryza sativa) to water deficit. Plant Science 163, 815-827. http://dx.doi.org/10.1016/S0168-9452(02)00217-0

Cao SQ, Ren G. 2009. The Role of Aminobutyric Acid in Enhancing Cadmium Tolerance in Arabidopsis thaliana. Russian in Fiziologiya Rastenii 56(4), 635–640. http://dx.doi.org/10.1134/S1021443709040190

De Pinto MC, Francis D, Gara L. 1999. The redox state of ascorbate– dehydroascorbate pairs, a specific sensor of cell division in tobacco By-Z cells. Protoplasma 209,90–97.

Dhindsa RS, Motowe W. 1981. Drought tolerance in two mosses,correlation with enzymatic defense against lipid peroxidation. Journal of Experimental Botany 32, 79–91.

Giannopolitis  CN.  Ries  SK.  1977.  Superoxide dismutase I. Occurrence in higher plants. Plant Physiol 59, 309-314. http://dx.doi.org/10.1104/pp.59.2.309

Guo Z, Ou W, Lu S, Zhong Q. 2006. Differential responces of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant physiology and Biochemistery 4,828-836. http://dx.doi.org/10.1016/j.plaphy.2006.10.024

Jakab G, Ton J, Flors V, Zimmerli L, Me´ traux JP, Mauch-Mani B. 2005. Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Plant Physiology 139,264–174. http://dx.doi.org/10.1104/pp.105

Jiang MY, Zhang JH. 2004.  Abscisic  Acid  and Antioxidant  Defense  in  Plant  Cells.  Acta  Botanica Sinica 46(1), 1-9. http://dx.doi.org/10.1007/s00709-010-0169-x

Khanna-Chopra R, Selote DS. 2007. Accimilation to drought stress generates oxidative stress tolerance in drought-resistant than susceptible wheat cultivar under field conditions. Environmental and Experimental Botany 60, 276-283.

Khanna-Chopra M, Khanna-Chopra R. 2004. Osmotic adjustment of chickpea in relation to seed yield and yield parameters. Crop Science 44, 449-455. http://dx.doi.org/10.2135/cropsci2004.4490

Kirigwi FM, Van Ginkel V, Trethowan R, Sears RG, Rajaram S, Paulsen GM. 2004. Evaluation of selection strategies for wheat adaptation across water regimes. Euphytica 135,61-371. http://dx.doi.org/10.1023/B:EUPH.0000013375.661 04.04

Krizek DT, Brita SJ, Miewcki RM. 1998. Inhibitoryeffects of ambient level of solar UV-A and UV-B on growth of cv. New Red Fire lettuce. Physiol Plant 103, 1-7.

Nakano Y, Asado K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5), 867–880. http://dx.doi.org/10.1111/j.1399-3054.1992.tb04728.x

Sairam RK, Shukla DS, Deshmukh PS. 1995. Effect of triazole triadimefon on tolerance to moisture stress in wheat (Triticum aestivum. Indian Journals of Agriculture Science 65, 483-489.

Ton J, Jakab G, Toquin V, Flors V, Iavicoli A, Maeder N, Me´ traux J, Mauch-Man B. 2005. Dissecting the b-Aminobutyric Acid–Induced Priming Phenomenon in Arabidopsis. The Plant Cell 17, 987– 999.

Ton J, Mauch-Mani B. 2004. Beta-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA dependent priming for callose. The Plant Journal 38, 119–130.

Wanger    GJ.    1979    Content   and    vacuole/extra vacuole  distribution  of  neutral  sugars,  free  amino acids, and anthocyanins in protoplast.Plant Physiol 64, 88–93 http://dx.doi.org/10.1007/978-1-4757-4727-0_1

Wu CC, Singh P, Chen MC, Zimmerli L. 2009. L-Glutamine inhibits beta-aminobutyric acid-induced stress resistance and priming in Arabidopsis. Journal of Experimental Botany 10, 1-8.

Zimmerli L, Hou BH, Tsai CH, Jakab G, Mauch-Mani B, Somerville S. 2008. The xenobiotic beta-aminobutyric acid enhances Arabidopsis thermotolerance. The Plant Journal 53, 144–156. http://dx.doi.org/10.1111/j.1365-313X.2007.03343.x

Zimmerli L, Jakab C, Metraux JP, Mauch-Mani B. 2000. Potentiation of pathogen-specific defense mechanisms in Arabidopsis by beta-aminobutyric acid. Proceedings of the National Academy of Sciences, USA 97, 12920–12925.

Zimmerli  L,  Me´  traux JP,  Mauch-Mani  B. 2001. beta-Aminobutyric acid-induced protection of Arabidopsis against the necrotrophic fungus Botrytis cinerea. Plant Physiology 126, 517–523.