Effect of bioaugmentation of crude oil polluted tropical soils on the growth of Panicum maximum

Paper Details

Research Paper 01/04/2015
Views (181) Download (9)

Effect of bioaugmentation of crude oil polluted tropical soils on the growth of Panicum maximum

Justina Chibuogwu Orji, Ifechukwu Enyinnaya Adieze, Rose Nkechinyere Nwabueze, Geoffrey Okike C. Onyeze
J. Bio. Env. Sci.6( 4), 227-235, April 2015.
Certificate: JBES 2015 [Generate Certificate]


The effect of bioaugmentation of crude oil polluted soils on the growth of Panicum maximum was examined in a green house study. Weathered polluted soil samples (2% w/w) in experimental pots were planted and inoculated with hydrocarbon utilizing bacterial species (Micrococcus sp. RZ1, Pseudomonas sp. RZIII, Bacillus sp. RZIII, and Bacillus sp. GSIII). Samples of unpolluted soil and polluted soil (2% w/w) were also maintained as controls. At intervals from the second to the tenth week after planting (WAP), three replicates each of the plants in soil samples with different treatments were randomly chosen, and then analyzed for plants’ shoot height, plants’ biomass and plants’ leaf area. The results of the study showed that the inoculation of polluted soils with competent hydrocarbon utilizing species offered some advantages to growth stimulation. The shoot height, shoot weight and root weight of P. maximum in polluted soil bioaugmented with Pseudomonas sp. RZIII and Bacillus sp. RZIII increased significantly more than those in polluted soil samples 10 WAP. The result highlights the importance of bioaugmentation with indigenous bacterial isolates that have adapted to the environment of application.


Adieze IE. 2012. Effect of bioaugumentation on soil microbial populations and residual crude oil concentration of a polluted tropical soil. Journal of Nigerian Environmental Society 7.

Adieze IE, Nwabueze RN, Onyeze GOC. 2003. Effect of poultry manure on the microbial utilization of hydrocarbons in oil–polluted soil. Nigerian Journal of Microbiology 17, 12 – 16.

Adieze IE, Orji JC, Nwabueze RN, Onyeze GOC. 2012. Hydrocarbon stress response of four tropical plants in weathered crude oil contaminated soil in microcosms. International Journal of Environmental Studies 69, 490-500.

Amadi A, Bari YU. 1992. Use of poultry manure for the amendment of oil polluted soils in relation to growth of maize. Environmental International 18, 521-527.

Atlas RM. 1995. Bioremediation of petroleum pollutants. International Biodeterioration and Biodegradation 35, 317-327.

American Public Health Association. 1985. Standard methods for water and waste water analyses, 16th ed. Washington D.C.

Banks MK, Govindaraju RS, Schwab AP, Kulakow P. 2000. Part I; Field demonstration. pp 3-88. In: Fiorenza, S., C.L. Oubre and C.H. Ward (ed). Phytoremediation of hydrocarbon-contaminated soil. Lewis Publishers, Boca Raton, Fl.

Bentos FM, Camargo FAO, Okeke BC, Frankenberger Jr WT. 2003. Bioremediation of soil contaminated by diesel oil. Brazilian Journal of Microbiology 34, 65-68.

Corgié SC, Beguiristain T, Leyval C. 2004. Spatial distribution of bacterial communities and phenanthrene degradation in the rhizosphere of Lolium perenne L. Applied Environmental Microbiology 70, 3552-3557.

Das N, Chandran P. 2011. Microbial Degradation of Petroleum hydrocarbon contaminants: An Overview. Biotechnology Research International 2011, 1-13.

Diaz E. 2008. Microbial Biodegradation: Genomics and Molecular Biology, 1st ed. Caister Academic Press.

Glick BR. 2003. Phytoremediation: Synergistic use of plants and bacteria to clean up the environment. Biotechnology Advances 21, 383-393.

Glick BR, Pasternak JJ. 2003. Plant growth promoting bacteria. pp. 436–454. In: B. R. Glick and J. J. Pasternak (ed), Molecular Biotechnology; Principles and Applications of Recombinant DNA. 3rd ed., ASM Press, Washington, DC.

Liste H, Felgentreu D. 2006. Crop growth, culturable bacteria, and degradation of petrol hydrocarbons (PHCs) in a long-term contaminated field soil. Appl. Soil Ecol. 31, 43-52.

Merkl N, Schultze-Kraft R, Infante C. 2004. Phytoremediation in the tropics – The effect of crude oil on the growth of tropical plants. Bioremediat. J. 8, 177-184.

Mishra S, Jyot J, Kuhad RC, Lal B. 2001. In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Current Microbiology 43, 328-335.

Nweke CO, Mgbachi LC, Nwanganga C, Nwanyanwu CE. 2006. Heavy metal tolerance among hydrocarbon utilizing bacteria isolated from oil-contaminated soil. Nigerian Journal of Microbiology 20, 1057–1065.

Odokuma LO, Dickson AA. 2003. Bioremediation of a crude oil polluted Tropical rain forest soil. Global Journal of Environmental Sciences 2, 29-40.

Okpokwasili GC, Amanchukwu SC. 1988. Petroleum hydrocarbon degradation by Candida sp. Environmental International 14, 243 – 247.

Onwurah INE. 2003. An integrated environmental biotechnology for enhanced bioremediation of crude oil contaminated agricultural land. Bio-Research 1, 51-57.

Siciliano SD, Fortin N, Mihoc A, Wisse G, Labelle S, Beaumier D, Ouellette D, Roy R, Whyte LG, Banks MK, Schwab P, Lee K, Greer CW. 2001. Selection of specific endophytic bacterial genotypes by plants in response to soil contamination. Applied and Environmental Microbiology 67, 2469-2475.

Singh S, Gaur YD. 1995. Soil microbes as inducers of nod-gene expression. Indian Journal of Microbiology 35, 317–325.

Tesar M, Reichenauer TG, Sessitsch A. 2002. Bacterial rhizosphere populations of black poplar and herbal plants to be used for phytoremediation of diesel fuel. Soil Biology and Biochemistry 34, 1883-1892.