Effect of different pH and temperature levels on in vitro growth and sporulation of Phytophthora colocasiae, taro leaf blight pathogen

Paper Details

Research Paper 01/04/2014
Views (203) Download (3)

Effect of different pH and temperature levels on in vitro growth and sporulation of Phytophthora colocasiae, taro leaf blight pathogen

Tsopmbeng Noumbo Gaston R., Lienou Jules Appolinaire, Megatche Christien Jean P., Fontem Dominic Ajong
Int. J. Agron. Agri. Res.4( 4), 202-206, April 2014.
Certificate: IJAAR 2014 [Generate Certificate]


Taro leaf blight disease caused Phytophthora colocasiae Racib is the most destructive disease which can cause significant economic losses of taro. The research was aimed at examining the effect of temperature and pH on the in vitro growth and sporulation of the fungus. V8-agar medium was adjusted to various levels of pH by adding appropriate amount of sodium hydroxide or hydrochloric acid before autoclaving and incubation of the fungus took place at five different temperatures. Colony diameters of the fungus were measured every day for 7 days and the number of sporangia was assessed after 21 days. The results showed that a pH of 7 and a temperature of 27 °C were the optimum conditions for pathogen growth while those of sporulation were 6 and 18 °C respectively . It is suggested that these factors would play a role in disease development.


Bedi PS, Singh JP. 1972. Leaf blight of rose in the Punjap. Indian Phytopathology 25, 534 – 539.

Benson DM. 1984. Influence of pine bark, matric potential, and pH on sporangium production by Phytophthora cinnamomi. Phytopa-thology 74, 1359 – 1363.

Bilgrami KS, Verma RN. 1978. Physiology of fungi. Vikas Publishing House PVT.Ltd. Dehli. 478 – 550.

Brooks FE. 2005. Taro leaf blight. The Plant Health Instructor.http://www.apsnet.org/edcenter/intropp/l essons/fungi/, visited 17 December 2011.

Fullerton R, Tyson J.L. 2004. The biology of Phytophthora colocasiae and implication for its management and control. In: Secretariat of the Pacific Community (Ed.). Third Taro Symposium, 2003. Nadi, Fiji Islands, 107 – 111.

Kong P, Gary WM., John DL, Ross D, Richardson PA, Hong C. 2009. Zoosporic Tolerance to pH Stress and Its Implications for Phytophthora Species in Aquatic Ecosystems. Applied Environmental Microbiology 75(13), 4307 – 4314.

Guarino L. 2010. Taro leaf blight in Cameroon 2010. Agricutural Biodiversity Weblog. http/agro. biodiver.se/2010/07/taro-leaf-blight-in-Cameroon/ visited 15 May 2012.

Matheron ME, Matejka JC. 1992. Effects of temperature on sporulation and growth of Phytophthora citrophthora and P. parasitica and development of foot and root rot on citrus. Plant Disease 76, 1103 – 1109.

Milus EA, Line RF. 1980. Characterization of resistance to leaf rust in Pacific Northwest wheat lines. Phytopathology 70, 167 – 172.

Milus EA, Seyran E, McNew R. 2006. Aggressiveness of Puccina striiformis f. sp. triticiisolates in the south-central States. Plant Disease 90, 847 – 852.

Phillips D, Weste G. 1985. Growth rates of 4 Australian isolates of Phytophthora cinnamomi in relation to temperature. Transactions of the British Mycological Society 84, 183 – 185.

Sahu AK, Maheshwari SK, Sriram S, Misra, RS. 2000. Effects of temperature and pH on the growth of Phytophthora colocasiae. Annals of Plant Protection Sciences 8(1), 112 – 114.

Scot N, Brooks F, Teves G. 2011.Taro Leaf Blight in Hawai‘I. Plant Disease 71, 1 – 14.

Simpfendorfer S, Harden TJ, Murray M. 2001. Effect of temperature and pH on the growth and sporulation of Phytophthora clandestine. Australasian Plant Pathology 30, 1 – 5.

Tsopmbeng GR, Fontem DA, Yamde KF. 2012. Evaluation of culture media for growth and sporulation of Phytophthora colocasiae Racib., causal agent of taro leaf blight. International Journal of Biolgical Chemestry Sciences 6(4), 1566 – 1573.

Zentmyer GA, Klure LJ, Pond EC. 1979. The influence of temperature and nutrition on formation of sexual structures by Phytophthora cinnamomi. Mycologia 71, 55 – 67.