Effect of drought stress on Brassica crops and its mitigation by inoculation of PGPR

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Effect of drought stress on Brassica crops and its mitigation by inoculation of PGPR

Maimona Saeed, Noshin Ilyas, Abida Akram, Naveed Raja, Roomina Mazhar, Fatima Bibi, Wajiha Seerat, Sidra Kanwal, Nazima Batool
Int. J. Biosci.9( 6), 282-291, December 2016.
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Abiotic stress is one of major cause of reduction in growth and productivity of crops. Water deficient is drastic environmental stress that interacts with metabolic pathways and finally productivity of various plants. Members of family Brassicaceae (canola and mustard) are cultivated as oil yielding crops all over the world. Morphological and physiological parameters of plants are greatly affected by drought stress, which cause decline in productivity of crops. Water stress is responsible for significant reduction in the amount of linolenic acid and increases contents of glucosinolates in oil.  Adaptation of different strategies for efficient use of soil moisture and reduction in drastic effect of drought has been a focal point for agriculture. In this aspect, one important strategy for enhancing the size of plant in water deficient condition is use of Plant Growth Promoting Rhizobacteria as inoculum. Plant Growth Promoting Rhizobacteria are naturally occurring in the rhizosphere which improve size of plants by various direct and indirect methods. PGPR also have potential to impart resistance in agricultural plants against unfavorable condition and can mitigate the deleterious effect of drought by processes known to as induce systemic tolerance (IST). Inoculation of plants with Plant Growth Promoting Rhizobacteria improves the metabolic processes of plant and consequently enhances the crop productivity. Owing to its importance, the present review describes the impact of water deficit condition on various morphological and yield parameters of Brassica crops as well as its mitigation by using inoculum of Plant Growth Promoting Rhizobacteria.


Ali Q, Ashraf M, Anwar F. 2009. Physico-chemical attributes of seed oil from drought stressed sunflower (Helianthus annuus L.) plants. Grasas y Aceites 60, 475-481. http://dx.doi.org/10.3989/gya.021009

Anjum F, Yaseen M, Rasul E, Wahid A, Anjum S. 2003a. Water stress in barley (Hordeum vulgare L.). I. Effect on morphological characters. Pakistan Journal of Agricultural Sciences 40, 43–44.

Arshad M, Shaharoona B, Mahmood T. 2008. Inoculation with Pseudomonas spp. containing ACC-deaminase partially eliminates the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.). Pedosphere 18, 611-620.

Arzanesh M, Alikhani H, Khavazi K, Rahimian A, Miransari M. 2011. Wheat (Triticum aestivum L.) growth enhancement by Azospirillum sp under drought stress. World Journal of Microbiology and Biotechnology 27 (2), 197-205. http://dx.doi.org/10.1007/s11274-010-0444-1

Ashraf M, Shabbaz M, Ali Q. 2013. Drought induced modulation in growth and mineral nutrients in canola (Brassica napus). Pakistan Journal of Botany 45 (3), 93-98.

Aslam MN, Kailis GS, Bayliss LK, Speijers J, Cowling AW. 2009. Canola oil increases in polyunsaturated fatty acids and decreases in oleic acid in drought-stressed Mediterranean-type environments Plant Breeding 128, 348–355.

Ashraf M, and McNeilly T. 2004. Salinity tolerance in Brassica oilseeds. Critical Review of Plant Sciences 23, 157-174. http://dx.doi.org/10.1080/07352680490433286

Bano Q, Ilyas N, Bano A, Zafar N, Akram A, Hussain F. 2013. Effect of Azospirillum inoculation on maize under drought. Pakistan Journal of Botany 45, 13-20.

Bashan Y, Holguin G, de-Bashan A. 2004. Azospirillum-plant relationships: physiological, molecular, agricultural and environmental advances (1993-2003). Candian Journal of Microbiology 50, 521-577.

Bertrand H, Nalin R, Bally R.  Marel CCJ. 2001. Isolation and identification of the  most efficient plant growth-promoting bacteria associated with canola (Brassica napus). Biology and Biochemistry 21, 511-517.

Casanovas EM, Barassi AC, Sueldo JR. 2002. Azospirillum inoculation mitigates water stress effects in maize seedlings. Cereal Research Comminication 30, 343- 349.

Chanway CP, Hynes RK, Nelson LM. 1989. Plant growth-promoting rhizobacteria: Effects on growth and nitrogen fixation of lentils and pea. Soil Biol Biochem 21: 511-517.

Creus CM, Sueldo JR, Barassi AC. 2004. Water relations and yield in Azospirillum inoculated wheat exposed to drought in the field. Candian Journal of Botany 82, 273-280.

Dickin E, Wright D. 2008. The effects of winter water logging and summer drought on the growth and yield of winter wheat Triticum aestivum L. European Journal of Agronomy 28, 234- 244.

Diepenbrock W. 2000. Yield analysis of winter rapeseed oilseed rape (Brassica napus L.), a review. Field Crops Research 67, 35-49. http://dx.doi.org/10.1016/S0378-4290(00)00082-4

Dreesen PE, De Boeck JH, Janssens AI, Nijs I. 2012. Summer heat and drought extremes trigger unexpected changes in productivity of a temperate annual/biannualplant community. Environmental and Experimental Botany 79, 21-30. http://dx.doi.org/10.1016/j.envexpbot.2012.01.005

Dwivedi SL, Nigam SN, Rao CRN, Singh U, Rao SVK. 1996. Effect of drought on oil, fatty acids and protein contents of groundnut (Arachis hypogaea L.) seeds Field Crop Research 48, 125-133. http://dx.doi.org/10.1016/S0378-4290(96)01027-1

Edward D. Wright D. 2008. The effects of winter water-logging and summer drought on the growth and yield of winter wheat (Triticum aestivum L.) European Journal of Agronomy 28, 234–244. http://dx.doi.org/10.1016/j.eja.2007.07.010

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra AMS. 2009. Plant drought stress: effects, mechanisms and management. Agronomy Sustainable Development 29, 185–212. http://dx.doi.org/10.1051/agro:2008021

Frommel MI, Nowak J, Lazarovits G. 1991. Growth enhancement and development modifications of in vitro grown potato (Solanum tuberosum sp.) as affected by a nonflurorescent Pseudomonas sp. Plant Physiology 96, 928-936. http://dx.doi.org/10.1104/pp.96.3.928.

Ghobadi M, Bakhshandeh M, Fathi G, Gharineh HM, Alamisaeed K, Naderi A, Ghobadi V. 2006. Short and long periods of water stress during different growth stages of canola (Brassica napus L.). Effect on yield, yield components, seed oil and protein contents. Journal of Agronomy 5, 336-341.

Glick BR, Patten LC, Holguin G, Penrose MD. 1999. Effect of abiotic stress on cereal crops. Journal of organic pollutant 24, 86-179.

Grover M, Ali ZS, Sandhya V, Rasul A, Venkateswarlu B. 2010. Role of microorganisms in adaptation of agriculture crops to abiotic stress. World Journal of Microbiology and Biotechnolgy 4, 445-449. http://dx.doi.org/10.1007/s11274-010-0572-7

Hong W, Ji-yun J. 2007. Effects of zinc deficiency and drought on plant growth and metabolism of reactive oxygen species in maize (Zea mays L.) Agricultural Science China 6, 988-995. http://dx.doi.org/10.1016/S1671-2927(07)60138-2

Huax XJ, Vande-Cotte B, Montayel MV, Verbragen N. 1997. Developmental regulation of proline-5- Carboxylate reductanse gens expression in arabidopsis. Plant Physiology 114, 1215-1224.

Kamara AY, Menkir A, Badu-Apraku B, Ibikunle O. 2003. The influence of drought stress on growth, yield and yield components of selected maize genotypes. Journal of Agricultural Sciences 141, 43–50.  http://dx.doi.org/10.1017/S0021859603003423

Kannaiyan S. 2002. Biofertilizers for sustainable crop production. In: Kannaiyan, S (ed)  Biotechnology of biofertilizera, Klawoer Academic Publishers, Dordrecht, The Netherland, 9-49.

Kastori R, Kadar I, Sekulić P, Bogdanović D, Milošević N, Pucarević M. 2006. Uzorkovanje zemljišta i biljaka nezagađenih i zagađenih staništa. Naučni institut za ratarstvo i povrtarstvo, Novi Sad.

Kloepper JW, Hume JD, Scher FM, Singleton C, Tipping B, Laliberté M, Frauley K, Kutchaw T, Simonson C, Lifshitz R, Zaleska I, Lee L. 1988. Effect of Plant Growth Promoting Rhizobacteria on canola (Rapeseed) Plant Disease 72, 42-4.

Kusaka M, Ohta M, Fujimura T. 2005. Contribution of inorganic components to osmotic adjustment and leaf folding for drought tolerance in pearl millet. Physiology of Plant 125, 474–489. http://dx.doi.org/10.1111/j.1399-3054.2005.00578.x

Lawlor DW, Cornic G. 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environment 25, 275–294.

Li C, Zhou A, Sang T. 2006. Rice domestication by reduced shattering. Science 311, 1936-1939. http://dx.doi.org/10.1126/science.1123604

Manivannan P, Jaleel AC, Sankar B, Kishorekumar A, Somasundaram R, Alagu Lakshmanan MG, Panneerselvam R. 2007b. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress Colloids and Surfaces B: Biointerfaces 59, 141–149. http://dx.doi.org/10.1016/j.colsurfb.2007.05.002

Manivannan P, Jaleel AC, Somasundaram R, Panneerselvam R. 2008. Osmoregulation and antioxidant metabolism in drought stressed Helianthus annuus under triadimefon drenching. Competus Rendus Biologies 331, 418–425. http://dx.doi.org/10.1016/j.crvi.2008.03.003

Milošević N. 2008. Mikroorganizmi – bioindikatori zdravlja / kvaliteta zemljišta. Zbornik radova Instituta za ratarstvo i povrtarstvo 45, 505–515.

Moaveni P, Ebrahimi A, Farahani AH. 2010. Studying of oil yield variation in winter rapeseed (Brassica napus L.)  cultivars under drough stress condition. J Agri Biotechnology Sustainable Development 2, 71-75.

Monneveux P, Sánchez C, Beck D. Edmeades OG. 2006. Drought tolerance improvement in tropical maize source populations: evidence of progress Crop Sciences 46, 180–191.

Mozaffari K, Arshi Y, Zeinali-Khanghaa H. 1996. Research on the effects of water stress on some morphophysiological traits and yield components of sunflower (Helianthus annuus L.) Seed Plant 12, 24–33.

Nasri M, Khalatbari M, Zahedi H, Paknejad F, Tohidi-Moghadam RH. 2008. Evaluation of micro and macro elements in drought stress condition in cultivars of rapeseed (Brassica napus L.). American Journal of Agriculture and Biological Sciences 3, 579– 583.

Nosheen A, Bano A, Ullah F. 2011. Nutritive value of canola (Brassica napus L.)   as affected by plant growth promoting rhizobacteria. European Journal of Lipid Science and Technology 113, 1342-1346. http://dx.doi.org/10.1002/ejlt.201000549

Petropoulos SA, Daferera D, Polissiou GM, Passam CH. 2008. The effect of water deficit stress on the growth, yield and composition of essential oils of parsley. Scientia Horticulturae 115, 393–397. http://dx.doi.org/10.1016/j.scienta.2007.10.008

Prabhudeva TV, Chalapathi MM, Thimmegowda S, Devakhumar N, Rao GG, Mallikarjuna K. 1998. Soil moisture stress and drought susceptibility index in sunflower. Indian Agriculture 42, 287–289.

Qaderi MM, Kurepin LV, Reid DM. 2006. Growth and physiological responses of canola (Brassica napus) to three components of global climate change: temperature, carbon dioxide and drought. Journal of Plant Physiology 128, 710–721. http://dx.doi.org/10.1111/j.1399-3054.2006.00804.

Rahnema AA, Bakhshandeh AM. 2006. Determination of optimum irrigation level and compatible canola varieties in the Mediterranean environment. Asian Journal of Plant Sciences 5, 543-546.

Reddy AR, Chaitanya VK, Vivekanandan M. 2004. Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology 161, 1189–1202.  http://dx.doi.org/10.1016/j.jplph.2004.01.013

Rodriguez H, Gonzales T, Goir I, Bashan Y. 2004. Gluconic acid production and phosphate solubilization by the plant growth promoting bacterium Azospirillum spp. Naturwissensechaften 91, 552-555. http://dx.doi.org/10.1007/s00114-004-0566-0

Rollins JA, Habte E, Templer ES, Colby T, Schmidt J, von Korff M. 2013. Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.). Journal of Experimental Botany 64, 3201-3212. http://dx.doi.org/10.1093/jxb/ert158

Sekhon HS, Singh G, Sharma P, Bains TS. 2010. Water Use Efficiency Under Stress Environments In: Climate Change and Management of Cool Season Grain Legume Crops (Eds S.S. Yadav, D.L. Mc Neil, R. Redden, and S.A. Patil). Springer Press, Dordrecht-Heidelberg-London-New York.

Shafi M, Raziuddin JB, Zhang G. 2009. Effect of Cadmium and Salinity Stresses on Growth and Antioxidant Enzymes Activity of Wheat Genotypes. Bulletin of   Environment Contimination and Toxicology 82, 772-776.

Sharp RE, and LeNoble EM. 2002. ABA, ethylene and the control of shoot and root growth under water stress. Journal of Experimental Botany 53, 33–7.

Shukla RK, Arvind K, Mahapatra SB, Basanth K. 2002. Integrated nutrient management practices in relation to morphological and physiological determination of seed yield in Indian mustard (Brassica napus L.). Indian Journal of Agriculture Sciences 72, 670-672.

Sieling K, Christen O, Nemati B, Hanus H. 1997. Effects of previous cropping on seed yield and yield components of oil-seed rape (Brassica napus L.). European Journal of Agronomy 6, 215-223. http://dx.doi.org/org/10.1016/S11610301(96)02049-7

Sinaki JM, Majidi Heravan E, Shirani Rad HA, Nour Mohammadi  G, Zarei H. 2007. The effects of water deficit during growth stages of canola (B. napus L.) American-Eurasian Journal of Agronomy Environment Sciences 2, 417–422.

Soriano MA, Villalobos JF, Fereres E. 2002. Stress timing effects on sunflower harvest index. In: Villalobos, F.J. and L. Testi (eds.). VII Congress of the European Society for Agronomy, 141–2.

Specht JE, Chase K, Macrander M, Graef LG, Chung J, Markwell PJ, Germann M, Orf HJ, Lark GK. 2001. Soybean response to water. A QTL analysis of drought tolerance. Crop Sciences 41, 493–509.

Tahir MHN, Mehid SS. 2001. Evaluation of open pollinated sunflower (Helianthus annuus L.) populations under water stress and normal conditions. International Journal of Agricultural Biology 3, 236–238.

Tahir M, Asghar A, Nadem AN, Tanveerans A, Sabir MQ. 2007. Performance of canola (Brassica napus L.) under different irrigation levels. Pakistan Journal of Botany 39, 739-746.

Tohidi-Moghadam HR, Shirani-Rad HA, Nour-Mohammadi G, Habibi D,  Mashhadi-Akbar-Boojar M. 2009. Effect of Super Absorbent Application on Antioxidant Enzyme Activities in Canola (Brassica napus L.) Cultivars under water stress condition, American Journal of Agriculture and Biological Sciences 4, 215-223.

Toorchi M, Shashidhar EH, Sharma N, Hittalmani S. 2002. Tagging QTLs for maximum root length in rainfed lowland rice (Oryza sativa L.) using molecular markers. Cellular & Molecular Biology Letters 7, 771-776.

Turan M, Esringu A. 2007. Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn. Plant, Soil and Environment 53, 7–15.

Vadez V, Berger DJ, Warkentin T, Asseng S, Ratnakumar P, Rao CPK, Gaur MP,   Munier-Jolain N, Larmure A, Voisin SA, Sharma CH, Pande S, Sharma M,  Krishnamurthy L. Zaman AM. 2012.Adaptation of grain legumes to climate change: a review. Agronomy Sustainable Development 32, 31-44. http://dx.doi.org/10.1007/s13593-011-0020-6

Vadez V, Kholova J, Choudhary S, Zindy P, Terrier M, Krishnamurth L, Kumar RP, Turner CN. 2011. Whole plant response to drought under climate change. In: Crop adaptation to climate change (Eds S.S. Yadav, R. Redden, J.L. Hatfield, H. Lotze-Campen, A.E. Hall). Chichester-Wiley-Blackwell.

Wahid A, Gelani S, Ashraf M, Foolad RM. 2007. Heat tolerance in plants: an overview. Environmental and Experimental Botany 6,199-223. http://dx.doi.org/10.1016/j.envexpbot.2007.05.011

Webber M, Barnett J, Finlayson B, Wang M. 2006. Pricing China’s Irrigation Water. Working Paper, School of Anthropology, Geography and Environmental Studies, The University of Melbourne, Victoria, Australia.

Xu DQ, Huang J, Guo QS, Yang X, Bao MY, Tang JH, Zhang SH. 2008. Over-expression of a TFIII-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). Federation of European Biochemical Societies Letters, 582, 1037- 1043. http://dx.doi.org/10.1016/j.febslet.2008.02.052.

Yasari E, Esmaeli A, Mozafari EAM, Alashti RM. 2009. Enhancement of growth and nutrient uptake of rapeseed (Brassica napus L.) by applying mineral nutrients and bio-fertilizers. Pakistan Journal of Biological Sciences 12, 127-133. 

Zhang M, Duan L, Zhai Z, Li J, Tian X, Wang B, He Z, Li Z. 2004. Effects of plant growth regulators on water deficit-induced yield loss in soybean. Proceedings of the 4th International Crop Science Congress, Brisbane, Australia.

Zhu Y, Cao Z, Xu F, Huang Y, Chen M, Guo W, Zhou W, Zhu J. Meng J, Zou J, Jiang L. 2012: Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.). Theory of Applied Genetics 124, 515—531. http://dx.doi.org/10.1007/s00122-011-1725-2