Effect of drought stress on germination in 18 population of thyme (Thymus kotschyanus)

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Research Paper 01/12/2015
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Effect of drought stress on germination in 18 population of thyme (Thymus kotschyanus)

Abbas Pourmeidani, Ali Ashraf Jafari, Hasan Maddah Arefi, Sepideh Torabi, Mahdi Mirza
J. Bio. Env. Sci.7( 6), 121-126, December 2015.
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Thyme is an important medicinal plant of Iran that have Thymol and Carvacrol and used for antimicrobial and antibacterial agent. The present study was carried out to determine the effect of drought stress in 18 populations of this species. The environmental stress such as, drought are serious obstacles for medicinal plants and field crops in further areas of the world, especially arid and semiarid regions. To create drought stress, polyetylenglycol (PEG) 6000 in osmotic levels at 0 (as control), -0.2, -0.4, -0.6 and -0.8 MPa were used. Effect of drought levels on investigated traits significant at 0.01. Result showed significance different between evaluated indices. Increasing of stress levels lead to reduction of germination and root and shoot length. Comparison of traits in the populations and different levels PEG application showed the highest germination in the population of Zanjan 2, Unknown 2 and Zanjan 4, respectively. For this trait in normal conditions and severe stress, there was no major difference between T. kotschyanus population. In terms of the radicle length, population of Zanjan 2 and Ghazvin 3 were higher than others. There was a lot variety in seed vigor. Population of Zanjan 2, Ghazvin 3 and Zarand were higher than others. Accessions Zanjan 2, Az. Gharbi 1, Qazvin 3, Zanjan 4 and Qazvin 2 in the majority of traits related to germination, your positive characteristics somewhat maintain in different levels of PEG stress . Accession combination of the above can use as a mass suitable for cultivation in rainfed and low yields rangelands.


Ahmad S, Ahmad R, Ashraf MY, Ashraf M, Waraich EA. 2009. Sunflower (Helianthus annuus L.) response to drought stress at germination and growth stages. Pakistan Journal Botany 41(2), 647-654.

Albuquerque FMD, Carvalho ND. 2003. Effect of type of environmental stress on the emergence of sunflower (Helianthus annuus L.), soybean (Glycine max) and maize (Zea mays L.) seeds with different levels of vigor. Seed Science Technology 31, 465- 467.

Bagheri M, Yeganeh H, Zandi E. 2011. Effects of water stress on seed germination of Thymus kotschyanus and Th. Daenensis. Middle –East Journal of Scientific Research 8(4), 726-731.

Cheng Z, Bradford KJ. 1999. Hydrothermal time analysis of tomato seed germination responses to priming treatments. Journal of Experimental Botany 33, 89-99.

Elhabazi K, Ouacherif A, Abbad A, Chait A, Dalal A. 2008 . Analgesic activity of three thyme species (Thymus satureioides) and (Thymus maroccanus). Journal of Microbiology Research 2, 262-267.

Ismaili H, Milella L, Frih-Tetouani S, Ilidrissi A, Camporese A, Aquino R. 2004. In vitro topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves. Journal of Ethno pharmacology 91, 31-36.

Khoshsokhan F, Babalar M, Fatahi MR. 2012. Effect of salinity and drought stress on germination indices of two Thymus species. Cercetari Agronomice in Moldova. Vol. XLV, No. 1 (149), 27-35.

Maguire JD. 1962. Speed of germination-Aid in selection and evaluation for seedling emergence and vigor. Crop Science 2, 176–177.

Maksimovic Z, Stojanovic D, Sostaric I, Dajic Z. 2008. Composition and radical-scavenging activity of Thymus glabrescens Wild. (Lamiaceae) essential oil Journal of the Science of Food and Agriculture 88, 2036-2041.

Munns R, Weir R. 1981. Contribution of sugars to osmotic adjustment in elongating and expanding zones of wheat leaves during moderate water deficits at two light levels.

Nayar H, Gupta D. 2006. Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environment Express Botany 58, 106-113.

Timmermans K. 2003. Intellectual property rights and traditional medicine: policy dilemmas at the interface. Social Science Medical 57, 745-756.

Zschocke S, Rabe T, Taylor JL. 2000. Plant part substitution- a way to conserve endangered medicinal plants. Journal Ethno pharmacology 71, 281-292.