Effect of exogenous application of salicylic acid on the growth, photosynthesis and proline content on maize in salt stress

Paper Details

Research Paper 01/05/2014
Views (209) Download (7)

Effect of exogenous application of salicylic acid on the growth, photosynthesis and proline content on maize in salt stress

Davar Molazem, Ali Bashirzadeh, Maryam Fathollahzadeh Ardabili
J. Bio. Env. Sci.4( 5), 46-52, May 2014.
Certificate: JBES 2014 [Generate Certificate]


To evaluate the effect of salt stress and salicylic acid application on growth and physiological traits of maize varieties, an experiment was conducted in factorial split plot based on RCBD design with 3 replications in research farm of Islamic Azad University of Ardebil branch during 2012-13. Salt stress factor including three levels (control, 50mM and 100mM NaCl) and acid salicylic (control, 1mM and 2mM). Results from the experiment showed that, between different salinity in plant height, chlorophyll a, leaf area index and proline were significantly different. Effect of salicylic except for chlorophyll a was not significant for all traits. With the increase of salt in the soil, plant height was significantly reduced. Minimum plant height in the third of salinity with 111 cm was obtained that with the dose of 50 mM had no significant difference. Leaf relative water content decreased with increasing salt, but this decrease was not significant. Between of salinity 50 to 100 mM, a significant difference was not found. But the least amount of chlorophyll a in 100 mM of salinity with 0.2001 mg chlorophyll per g fresh weight of leaves was obtained. Leaf area decreased with increasing salinity. Highest proline with 1.351 mmol g fresh weight of leaves was obtained in normal conditions. Between chlorophyll a with chlorophyll b, significant positive correlation was obtained. But with proline a non-significant negative correlation was found. Between leaf area index with leaf relative water content, chlorophyll a and chlorophyll b was seen a significant positive correlation


Abdel-Wahed MSA, Amin AA, El-Rashad, SM. 2006. Physiological effect of some bioregulators on vegetative growth, yield and chemical constituents of yellow maize plants. World Journal Agriculture Science 2(2), 149-155. dx.doi.org/WJAS/2. P 149-155.

Ahmad, I, Khaliq T, Ahmad A, Basra SMA, Hussain Z, Ali A. 2012. Effect of seed priming with ascorbic acid, salicylic acid and hydrogen peroxide on emergence, vigor and antioxidant activities of maize. African Journal Biotechnol 11, 1127–1132. dx.doi.org/AJB/11. P 1127-1132.

Amin AA, Rashad ElShM, Hassanein MS, Zaki M. 2007. Response of Some White Maize Hybrids to Foliar Spray with Benzyl Adenine. Research Journal Agriculture Biological Sciences 3(6), 648-656. dx.doi.org/RJABS/3. P 648-656.

Andersen PP, Pandya-Lorch R, Rosegarnt MW. 1999. World food prospects: Critical issues for the early twenty-first century. 2020 Vision Food Policy Report, International Food Policy Research Institute,Washington, D.C.

Ashraf M, Athar HR, Harris PJC, Kwon TR. 2008. Some prospective strategies for improving crop salt tolerance. Advance Agronomy 97, 45–110. dx.doi.org/AA/97. P 45-110.

Ashraf M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advance 27, 84–93. dx.doi.org/BA/27. P 84-93.

Azizi Yegane M. 2010. ‘The use of a variety of grape seed Acid salicylic on quality and storage life of white’, MSc thesis Horticultural Science – University of Agriculture, Karaj Branch .Iran. (In Persian)

Bates LS, Waldren RP, Teare LD. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205-207. dx.doi.org/PS/39. P 205-207.

Cha-um S, Pokasombat Y, Kirdmanee C. 2011. Remediation of salt-affected soil by gypsum and farmyard manure − Importance for the production of Jasmine rice. Australian Journal Crop Sciences 5, 458-465. dx.doi.org/AJCP/5. P 458-465.

Chen Z, Zheng Z, Huang J, Lai Z, Fan B. 2009. Biosynthesis of salicylic acid in plants. Plant Signaling Behaviour 4, 493–496. dx.doi.org/PSB/4. P 493-496.

Farooq M, Aziz T, Basra SMA, Cheema MA, Rehman H. 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. Journal Agronomy Crop Sciences 194, 161–168

Iqbal M, Ashraf M. 2006. Wheat seed priming in relation to salt tolerance, growth, yield and level of free salicylic acid and polyamines. Annals’ Botanical Financial 43(4), 250-259.

Jamil A, Riaz S, Ashraf M, Foolad MR. 2011. Gene expression profiling of plants under salt stress. Crit Rev Plant Sciences 30, 435-458. dx.doi.org/CRPS/30 P 435-458.

Kaya C, Tuna AL, Okant AM. 2010. Effect of foliar applied kinetin and indole acetic acid on maize plants grown under saline conditions. Turk Journal Agronomy 34, 529-538

Khan A. 2007. Influence of exogenously applied ascorbic acid on growth and physiological attributes of hexaploid wheat (Triticum aestivum L.) under salt stress. Ph. D. Dissertation. Department of Botany, University of Agriculture, Faisalabad, Pakistan

Khan NA, Syeed S. Masood A, Nazar R, Iqbal N. 2010. Application of salicylic acid increases the contents of nutrient metabolism in mungbean and alleviate adverse effects of salinity. International Journal Plant Biology.

Khan W, PrJrithivira B, Smith A. 2003. Photosynthetic responsesof corn and soybean to foliar application of salicylates. Journal Plant Physiology 160, 485–492. dx.doi.org/JPP/160 P 485-492.

Krasensky J, Jonak C. 2012. Drought, salt, and temperature stress-induced metabolic rearrange-ments and regulatory networks. Journal Express Botany 63, 1593-1608. dx.doi.org/JEB/63 P 1593-1608.

Molazem D, Azimi J, Ghasemi M. 2012. Investigation of the effect of salt stress on the antioxidant enzyme activities on the leaves Maize (ZEA MAYS L.).wulfenia journal 19(9), Sep 2012.

Molazem D, Azimi J. 2011. Effect of Different Levels of Salinity on Leaf Characteristics and Chlorophyll Content of Commercial Varieties of Maize (Zea Mays L.). Australian Journal of Basic and Applied Sciences 5(12), 1718-1722. dx.doi.org/AJBAS/5 P 1718-1722.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual Revere Plant Biology 59, 651–681

Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60(3), 324-349

Sakr MT, Arafa AA. 2009. Effect of some antioxidants on canola plants grown under soil salt stress condition. Pakistan Journal Biology Sciences 12, 582–588. dx.doi.org/PJBS/12 P 582-588.

Sinha SK, Srivastava HS, Tripathi RD. 1993. Influence of some growth regulators and cations on inhibition of chlorophyll biosynthesis by lead in Maize. Bull. Env. Contamin. Toxic 51, 241–6

Wahid A, Perveen M, Gelani S, Basra SMA. 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal Plant Physiology 164, 283–294. dx.doi.org/JPS/164 P 283-294.

Yamasaki S, Dillenburg LC. 1999. Measurements of leaf relative water content in Araucaria angustifolia. R Bras Fisiol Veg 11, 69-75

Zhao HJ, Lin XW, Shi HZ, Chang SM. 1995. The regulating effects of phenolic compounds on the physiological characteristics and yield of soybeans. Acta Agronomy 21, 351–355. dx.doi.org/AA/21 P 351-355.