Effect of explants and growth regulators on the expression of Callogenesis, somatic embryogenesis and plantlets formation in Sugarcane (Saccharum officinarum L.)

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Research Paper 01/10/2016
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Effect of explants and growth regulators on the expression of Callogenesis, somatic embryogenesis and plantlets formation in Sugarcane (Saccharum officinarum L.)

Muhammad Iqbal, Aamir Ali, Naima Huma Naveed, Umair A. Khan, Muhammad Nasim Ashraf Faz, Muhammad Imran, Danish Ashfaq, Mubashir Hussain
Int. J. Biosci.9( 4), 147-156, October 2016.
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The present research was done to standardize a protocol for hormone treatment and to determine the best type of explants for the induction of callus, somatic embryogenesis and regeneration response in sugarcane. The potential for callogenesis and somatic embryogenesis of different explants of sugarcane was explored. The influence of different concentrations and combinations of various phytohormones along with varying environmental conditions like temperature and light on in vitro callus induction and somatic embryo formation was also studied. Among three types of explants used in present study (leaf, shoot apical meristem and pith), the maximum response of callus induction and somatic embryogenesis was obtained from leaf explants. The maximum callus induction response obtained onto MS medium containing 3 mg/l of 2, 4- D was 92% from leaf, 72% from shoot apical meristem and 50% from pith explants after 14 days of inoculation. Direct and indirect somatic embryogenesis was observed and the best response was obtained on MS medium supplemented with 3 mg/l 2, 4-D + 0.5 mg/l BAP. MS media supplemented with 4mg/l 2, 4-D+ 0.25mg/l BAP was proved to be the best for regeneration of somatic embryos, where 90% plantlets formation was observed. Thus, leaf explants must be cultured onto MS medium with 2, 4 D / BAP corresponding to 4 mg/l / 0.25 mg/l to optimize somatic embryogenesis and plantlets regeneration.


Ali A, Naz S, Iqbal J. 2007. Effect of different explants and media composition for efficient somatic embryogenesis in sugarcane (Saccharum oficinarum). Pakistan Journal of Botany 39(6), 1961-1977.

Ali S, Iqbal J, Khan MS. 2010. Genotype independent in vitro regeneration system in elite varieties of sugarcane. Pakistan Journal of Botany 42, 3783-3790.

Alves SAO, Lemos OFD, Filho S, Silva ALLD. 2011. In vitro embryo rescue of interspecific hybrids of oil palm (Elaeis oleifera x Elaeis  guineensis). Journal of Biotechnology. Biodiversity 2, 1-6.

Ather A, Khan S, Rehman A, Nazir M. 2009. Optimization of the protocols for callus Induction, regeneration and acclimatization of sugarcane cv. Thatta-10. Pakistan Journal of Botany 41(2), 815-820.

Behera KK, Sahoo S. 2009. Rapid In vitro Micro propagation of Sugarcane (Saccharum officinarum L. cv. Nayana) Through Callus Culture. Nature and Science 7(4), 1-3.

Biradar S, Biradar DP, Patil VC, Patil SS, Kambar NS. 2009. In vitro plant regeneration using shoot tip culture in commercial cultivar of sugarcane. Karnataka Journal of Agricultural Science 22(1), 21-24.

Desai N. S, Suprasanna P, Bapat VA. 2004. Simple and reproducible protocol for direct somatic embryogenesis from cultured immature inflorescence segments of sugarcane (Saccharum spp.). Current Science 87, 764-768.

Helal NAS. 2011. The green revolution via synthetic (artificial) seeds: A Review. Research Journal of Agricultural and Biological Sciences 7(6), 464-477.

Jahangir GZ, Nasir IA, Sial RA, Javid MA, Husnain T. 2010. Various Hormonal Supplementations Activate Sugarcane Regeneration  In vitro. Journal of Agricultural Sciences 2(4), 231-233.

Khan SA, Rashid H, Chaudhary MF, Chaudhry Z, Afroz A. 2008. Rapid micropropagation of three elite sugarcane (Saccharum officinarum L.) varieties by shoot tip Culture. African Journal of Biotechnolgy 7 (13), 2174-2180.

Latif Z, Nasir A, Riaz-ud-Din S. 2007. Endogenous production of synthetic seeds in Daucus carota. Pakistan Journal of Botany 39(3), 849-855.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiology Plantarum 15, 473-487. http://doi/10.1111/j.1399-3054.1962.tb08052

Naz S, Ali A, Siddique FA. 2008. Somatic embryogenesis and plantlet formation in different varieties of sugarcane (Sacchrum officinarum L.) HSF-243 and HSF-245. Sarhad journal of Agriculture 24(4).

Philips GC, Gamborg OL. 2005. Plant cell tissue & organ culture: Fundamental Methods. Published by N. K. Mehra, Narosa publishing house 6, community centre New Dehli, India.pp, 91-93.

Raja N, Abbas H. 2006. Sugarcane Overview, Http://www.pakistan/com/English/allabout/crop/sugarcane.shtml

Santosa DA, Hendroko R, Farouk A, Greiner R. 2004. A rapid and highly efficient method for transformation of sugarcane callus. Molecular Biotechnology 28, 113 -119.

Sengar K, Sengar RS, Garg SK. 2011. The effect of in vitro environmental conditions on some sugarcane varieties for micropropagation. African Journal of Biotechnology 10(75), 17122-17126. http://dx.doi.org/10.5897/AJB11.2195

Shah AH, Rashid N, Haider MS, Saleem S, Tahir M, Iqbal J. 2009. An efficient, short and cost-effective regeneration system for transformation studies of sugarcane (Saccharum officinarum L.). Pakistan Journal of Botany 41(2), 609-614.

Tabassum B, Nasir IA, Farooq AM, Rehman Z, Latif Z, Husnain T. 2010. Viability assessment of In vitro produced synthetic seeds of cucumber. African Journal of Biotechnology 9(42), 7026-7032. http://dx.doi.org/10.5897/AJB10.060

Thorpe T. 2007. History of plant tissue culture. Molecular Biotechnology 37, 169-180. http://dx.doi.org/10.1007/s12033-007-0031-3