Effect of foliar applied glycine betaine on growth performance of broccoli (Brassica oleracea var. Italica)

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Research Paper 01/12/2020
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Effect of foliar applied glycine betaine on growth performance of broccoli (Brassica oleracea var. Italica)

Sana Liaqat, Nida Mansoora, Muhammad Shahnawaz Bhatti, Shafaq Aslam, Nida Batool, Sana Ishaq, Ashir Masroor, Filza Ghafoor
Int. J. Biosci.17( 6), 287-292, December 2020.
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Glycine betaine performs a crucial role, like osmoprotectant and cytoplasmic osmotic solute specifically in the members of family Gramineae and Chenopodiaceae. Brassica oleracea var. Italica subvariety PALMIRA 2 hybrid due to the presence of glucosinolate plays a vital role for humans. A pot experiment was performed at the Old Botanical Garden, University of Agriculture, Faisalabad. 35 days seedlings were transplanted to pots filled with 7 kg of soil for two weeks to get them acclimatized to pot soil conditions. Foliar application of glycine betaine (0, water spray, 5, 10, and 15 mM) was given after 2 months of transplantation. Data regarding the growth parameters, reactive oxygen species, antioxidant enzymes, mineral ions determination, nutrient use efficiency, nutrient uptake, total soluble proteins, and photosynthetic pigments were collected after 15 days of glycine betaine application. Application of GB increased shoot fresh and dry weight, shoot length, root dry weight, shoot calcium, potassium, nutrient uptake of sodium, calcium, potassium, SOD, peroxidase, and total soluble proteins of Broccoli (Brassica oleracea var. Italica). Implementation of GB showed non-significant results of parameters such as root fresh weight, photosynthetic pigments (chlorophyll a, b, a/b, carotenoids, and total chlorophyll), nutrient use efficiency of sodium, and catalase activity.


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Abbreviations SOD Superoxide dismutase, POD peroxidase.