Effect of formalin on fertilization, hatching rate of eggs of Thai Pangas (Pangasius hypophthalmus) and survival and growth performance of fry

Paper Details

Research Paper 01/04/2019
Views (884) Download (20)

Effect of formalin on fertilization, hatching rate of eggs of Thai Pangas (Pangasius hypophthalmus) and survival and growth performance of fry

Md. Habibur Rahman, Syeda Maksuda Yeasmin, Md. Anisur Rahman, Md. Almamun Farid, Abdulla-Al-Asif
Int. J. Biosci.14( 4), 158-165, April 2019.
Certificate: IJB 2019 [Generate Certificate]


Eggs of matured Thai Pangas (Pangasius hypophthalmus) were ranging from 2500 to 4200g comparative to total body weight (TBW) which were treated with 0.5 ml and 1 ml formalin concentrations for 60 seconds to determine its efficacy and effect on fertilization, hatching of egg, survival and growth performance of fry in this present study. Each treatment was replicated three times in the experiment. The present research work was conducted at Mafatema fish hatchery, Chanchra, Jashore and laboratory of Fisheries and Marine Bioscience department, Jashore University of Science and Technology, Jashore, Bangladesh. In case of fecundity, mean fecundity did not differed significantly (P>0.05); 120580±9560, 134292±16035 and 117238±7986 per kg body weight for 0.5 ml (T2), 1 ml (T3) and control (T1) group respectively. The highest fertilization rate (93.68±5.78) and hatching rate (88.12±9.34) were found in T3 which were significantly different (P<0.05) with T1 and T2. This study revealed, T3 showed the highest survival rate (92.50%) that is significantly different from T1 and T2. The present study showed that, the growth performance of P. hypophthalmus was found higher such as percentage of weight gain 95.26 ± 12.01 and specific growth rate 0.65 ± 0.06 in T3 which were significantly higher than T1 and T2. Eggs of P. hypophthalmus treated with 1 ml diluted formalin concentration for 60 seconds showed better results in terms of fertilization and hatching rate of eggs, survival and growth performance of fry.


Akpoilih BU, Adebayo OT. 2010. Effect of formalin on the hatching rate of eggs and survival of larvae of the Thai pangas (P. hypophthalmus). Journal of Applied Science and Environmental Management 14(4), 31-34.

Ali MA, Mondal S, Asif AA, Billah MM. 2018. Present status of some selected hatcheries at Chanchra under Jashore district, Bangladesh: An overview. European Academic Research 6(6), 2694-2712.

Ali MM, AA Asif, Shabuj MAI, Faruq O, Vaumik S, Sharif BMN and Zafar MA. 2016b. Technology of artificial breeding of catfish species in the hatcheries in Jessore Region, Bangladesh. International Journal of Fisheries and Aquatic Studies 4(1), 180-188.

Ali MM, Asif AA, Shabuj MAI, Faruq O, Vaumik S, Zafar MA, Sharif BMN. 2016a. Dose optimization with synthetic hormone flash for induced spawning of Shing (Heteropneustes fossilis). International Journal of Fauna and Biological Studies 3(1), 39-45.

Barnes ME, Ewing DE, Cordes RJ, Young GL. 1998. Observation on hydrogen peroxide control of Saprolegnia spp. during Rainbow Trout egg incubation. The Progressive Fish-Culturist 60, 67-70. http://dx.doi.org/10.1577/1548-8640(1998)060<3C0067:OOHPCO>3E2.0.CO;2

Barnes ME, Gabel AC, Cordes RJ. 2000. Bacterial population during Rainbow Trout egg culture in vertical-flow tray incubators. North American Journal of Aquaculture 62, 48-53. https://doi.org/10.1577/1548-8454(2000)062<0048:BPDRTE>2.0.CO;2

Barnes ME, Stephenson H Gabel M. 2003. Use of hydrogen peroxide and formalin treatments during incubation of landlocked fall Chinook salmon eyed eggs. North American Journal of Aquaculture 65, 151-154. http://dx.doi.org/10.1577/1548-8454(2003)65<3C151:UOHPAF>3E2.0.CO;2

Blythe WG, Helfrich LA, Libey G. 1994. Induced maturation of striped bass, Morone saxatilis exposed to 6, 9 and 12 months photothermal regimes. Journal of World Aquaculture Society 25, 183-192. https://doi.org/10.1111/j.1749-7345.1994.tb0018.0.x

Crespi V, Coche A. 2008. Glossary of Aquaculture. Food and Agriculture Organization of the United Nations.

Goudie CA, Scinco BA, Davis KB, Parker NC. 1992. Reproductive performance of pigmented and Albino female Channel catfish induced to spawn with HCG or Ovaprim. Journal of World Aquaculture Society 23(2), 138- 145. https://doi.org/10.1111/j.1749-7345.1992.tb00762.x

Griffith D, Khanh PV, TrongTQ.2010. Cultured aquatic species information programme: Pangasius hypothalamus (Sauvage 1878). In: Fisheries and Aquaculture Department. FAO, Rome.

Grotmol S, Totland GK. 2000. Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactivates noda virus and increases survival of the larvae. Diseases of Aquatic Organisms 39, 89-96. https://doi.org/10.3354/dao039089

Hossain MT, Alam MS, Rahman MH, Asif AA and Rahmatullah SM. 2016. Present status of Indian major carp broodstock management at the hatcheries in Jessore region of Bangladesh. Asian-Australasian Journal of Bioscience and Biotechnology 1(2), 362-370.

Islam MM, Asif AA, Amin MR. 2016. The induced breeding of common carps (Cyprinus carpio) in Bangladesh. Indian Journal of Science 23(84), 619-632.

Islam MS, Asif AA, Sarker B, Satter A, Ahmed M, Rahman M, Zafar MA, Rahmatullah SM. 2017. Fry production and its marketing system of North-West fisheries extension project at Parbatipur, Dinajpur, Bangladesh. Asian Journal of Medical and Biological Research 3(3), 368-378. https://doi.org/10.3329/ajmbr.v3i3.34527

Jung C, Kim JW,  Jeon IG,  Lee YH. 2004. Formaldehyde residues in formalin treated olive flounder Paralichthysolivaceus, black rockfish Sebastes schlegeli. Aquaculture 194,251-262. https://doi.org/10.1016/S0044-8486(00)00530-5

Madsen L, Moller JD, Dalsgaard I. 2005. Flavobacterium psychrophilum in rainbow trout, Oncorhyncus mykiss Walbaum hatcheries: studies on brood stock eggs, fry and environment. Journal of Fish Diseases 28, 39-47. https://doi.org/10.1111/j.1365-2761.2004.00598.x

Miguez B, Combaro MP. 2003. Disinfection of striped trumpeter Latris lineate eggs with glutaraldehyde. Aquaculture International 9, 189-196.

Milton J, Haniffa MA, Paulasir JS, Kumaresan K, Jafar S. 2015.Hatching rate of striped murrel Channa striatus (Bloch 1797) eggs treated with formalin, hydrogen peroxide and copper sulphate pentahydrate. Indian Journal of Animal Sciences 85 (2), 216–217.

Moses TLS, Felix S, Samuel, Thiruvengadam V. 2016. Induced breeding, egg and embryonic development of Pangasianodon hypophthalmus (Sauvage, 1878) under hatchery conditions of north Tamil Nadu (Chennai). Indian Journal of Animal Sciences 67, 388-392.

Neowajh MS, Rashid MM, Asif AA, Zafar MA, Hossain A. 2017. Effects of chemotherapeutics against experimentally injured stinging catfish Heteropneustes fossilis. Asian Journal of Medical and Biological Research 3(4), 476-487. https://doi.org/10.3329/ajmbr.v3i4.35338

Oyelese OA. 2006. Water temperature a determinant of fertilization and hatchability rates in artificially induced breeding of P. hypophthalmus. Research Journal of Biological Sciences 14, 83-87.

Peck MA, Buckley L, Bryan O, Davies EJ, Lapolla AE. 2004. Efficacy of egg surface disinfectants in captive spawning Atlantic cod Gadus morhua and haddock Melanogrammus aeglenus. Aquaculture Research 35, 992-996. https://doi.org/10.1111/j.13652109.2004.01119.x

Pedersen LF, Pedersen PB, Nelson JL, Nelson PH. 2008. Long term/low dose formalin exposure to small- scale aquaculture systems. Aquacultural Engineering 42, 1-7. https://doi.org/10.1016/j.aquaeng.2009.08.002

Rahman MA, Rahman MH, Yeasmin SM, Asif  AA, Mridha D. 2017. Identification of causative agent for fungal infection and effect of disinfectants on hatching and survival rate of bata (Labeo bata) larvae. Advances in Plants and Agricultural Research 7, 00264. https://doi.org/10.15406/apar.2017.07.00264

Salvesen I, Ovie G, Vadstein O. 1997. Surface disinfection of Atlantic halibut and turbot eggs with glutaraldehyde: evaluation of concentrations and contact times. Aquaculture International 5, 249-258. https://doi.org/10.1023/A:1018343602872

Shabuj MAI, Asif AA, Faruq O, Bari MR, Rahman MA, 2016. Brood stock management and induced breeding of Thai Pangus (Pangasius hypophthalmus) practiced in the hatcheries of Jessore region, Bangladesh. International Journal of Business, Social and Scientific Research 4(4), 235-246.

Sharif BMN, Asif AA. 2015. Present status of fish hatchlings and fry production management in greater Jessore, Bangladesh. International Journal of Fisheries and Aquatic Studies 2(5), 123-127.

Vidthayanon C, HoganZ. 2013. Pangasianodon hypophthalmus: The IUCN Red List of Threatened Species. IUCN, Gland, Switzerland.

Yeasmin SM, Rahman MA, Hossain MMM, Rahman MH, Asif AA. 2016. Identification of causative agent for fungal infection and effect of disinfectants on hatching and survival rate of common carp (C. carpio) larvae. Asian Journal of Medical and Biological Research 1(3), 578-588. https://doi.org/10.3329/ajmbr.v1i3.26481

Yisa TA, Tsadu SM, Aidoo T, Ibrahim A, Gana ES, Adama BS. 2014. Effect of chemical disinfectant (formalin) on hatching of eggs of African catfish (Clarias gariepinus), survival and growth performance of fry. International journal of current microbiology and applied sciences 3(3), 1133-1138.

Yisa TA, Tsadu SM, Musa A. 2010. Effect of Nematode infection on the breeding potential of P. hypophthalmus. Journal of Agriculture, Forestry and the Social Sciences 8(1), 141-147.

Yisa TA. 2012. Induced breeding of P. hypophthalmus and Heterobranchus bidorsalisusing Non-Conventional methods of Abdominal Incision and Stripping of Males. Unpublished.