Effect of gamma radiation and potassium sorbate on sensory evaluation, chemical and microbial analysis of poa (Pama pama) preserved at low temperature

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Effect of gamma radiation and potassium sorbate on sensory evaluation, chemical and microbial analysis of poa (Pama pama) preserved at low temperature

Md. Shajadul Islam, M. Kamruzzaman Munshi, Roksana Huque, Arzina Hossain, Mst. Afifa Khatun, Mahfuza Islam, Md. Mazibur Rahman, Md. Shafiqul Islam Khan
Int. J. Biosci.15( 2), 78-91, August 2019.
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Poa is an economical important fish species in southern regionof Bangladesh. The study was carried out to evaluate the effectiveness of gamma radiation (1.0 and 1.5 kGy) and 2% potassium sorbate (dipped in 30 and 60 seconds) at low temperature (±4°C) on sensory quality, biochemical composition, microbial load and shelf-life of poa (Pama pama). According to control panel, the organoleptic scores gradually decreased with the progress of storage time. Irradiated and potassium sorbate (2%) treated samples showed the highest acceptable score (5) up to 28 and 21days respectively. Tyrosine value was lower in irradiated (1.5 KGy) sample than potassium sorbate (2%) treated sample and significantly lower than control. Irradiation has led to a significant reduction in total number of microbes. The highest total bacterial count was observed 9.4×107 cfu/g (14th day) in control sample. The highest total coliform and staphylococcus species count was 3.2×104 cfu/g (14th day) and 4.15×104 cfu/g (0th day) respectively in control sample. Bacteria were identified based on their cultural and biochemical characters. Lactococcus lactis, Klebsiella, E. coli and Enterobacter were sensitive to all the tested 10 antibiotics except erhromycin and penicillin. All were highly sensitive to ciprofloxacin and chloramphenicol and resistant to erhromycin and penicillin. B.cereus, Stephylococcus, Pseudomonas, Micrococcus and P. aeroginosawere resistant except Stephylococcus to gentamicin and imipenem. Study of antibiogram revealed multi-drug resistance of some of the isolates. Combination with irradiation (1.5 KGy) and low temperature (±4ºC) could be most effective treatment in extending shelf-life and overall reductions in microbial load of poa.


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