Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]

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Research Paper 25/11/2022
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Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]

P. Manivel, V. Prabakaran, S. Parvathi, S. Palanivel
J. Bio. Env. Sci.21( 5), 268-274, November 2022.
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The present study attempts to evaluate the mutagenic effect of gamma rays on okra cultivars of Arka anamika and Arka abhay. The seeds of two traditional cultivars of okra Arka anamika and Arka abhay were treated with different doses of gamma rays like 100, 200, 300, 400, 500 and 600Gy. The gamma irradiation influenced the growth, yield parameters, protein content and banding pattern in M1 generation. The M1 growth parameters like germination percentage, survival percentage, plant height and number of leaves per plant and yield parameters such as number of fruits per plant, fruits length, fruit weight, number of seeds per fruit and 100 seeds weight were measured. When compared to control, all the growth and yield parameters were decreased with increasing doses of gamma rays in M1 generation. The SDS-PAGE analysis of M1 generation leaf samples of treated population and control showed the appearance and disappearance of some protein bands and the Arka anamika was responded well than Arka abhay in all the terms studied between the two respectively.


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