Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]
Paper Details
Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]
Abstract
The present study attempts to evaluate the mutagenic effect of gamma rays on okra cultivars of Arka anamika and Arka abhay. The seeds of two traditional cultivars of okra Arka anamika and Arka abhay were treated with different doses of gamma rays like 100, 200, 300, 400, 500 and 600Gy. The gamma irradiation influenced the growth, yield parameters, protein content and banding pattern in M1 generation. The M1 growth parameters like germination percentage, survival percentage, plant height and number of leaves per plant and yield parameters such as number of fruits per plant, fruits length, fruit weight, number of seeds per fruit and 100 seeds weight were measured. When compared to control, all the growth and yield parameters were decreased with increasing doses of gamma rays in M1 generation. The SDS-PAGE analysis of M1 generation leaf samples of treated population and control showed the appearance and disappearance of some protein bands and the Arka anamika was responded well than Arka abhay in all the terms studied between the two respectively.
Adamu AK, Aliyu H. 2007. Morphological effects of sodium azide on tomato (Lycopersicon esculentum Mill.). Science World Journal 2(4), 9-12.
Balakrishnan PC. 1991. Induced mutagenesis in soybean (Glycine max (L.) Merrill) Ph.D. Thesis, Tamil Nadu Agriculture University, Coimbatore.
Benchasri S. 2012. Okra (Abelmoschus esculentus (L.) Moench) as a valuable vegetable of the world. Ratarstvoi Povrtarstvo 49, 105-112.
Dubey P, Mishra S. 2017. A review on: Diabetes and okra [Abelmoschus esculentus (L.) Moench]. Journal of Medicinal Plants Studies 5, 23-26.
Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F. 2015. Nutritional Quality and Health Benefits of Okra (Abelmoschus esculentus): A review. Journal of Food Process Technology 6, 458.
Gnanamurthy S, Dhanavel D, Bharathi T. 2013. Effect of chemical mutagenesis on biochemical activity and variability, heritability and genetic advance in Zea mays (L) International Journal of Current Science 5, 57-61.
Gnanamurthy S, Mariyammal S, Dhanavel D, Bharathi T. 2012. Effect of gamma rays on yield and yield components characters R3 generation in cowpea (Vigna unguiculata (L.). Walp.). International Journal of Research and Plant Science 2(2), 39-42.
Hegazi AZ, Hamideldin N. 2010. The effect of gamma irradiation on enhancement of growth and seed yield of okra [Abelmoschus esculentus (L.) Moench] and associated molecular changes. Journal of Horticulture and Forestry 2(3), 038-051.
Jadhav PA, Kalpande HV, Abad SK, Mali AR. 2013. Induced mutagenesis in okra [Abelmoschus esculentus (L.) Moench] by gamma rays and ethyl methane sulphonate. Vegetable Science 40(2), 223-224.
Jagajanantham N, Dhanavel D, Pavadai P, Chidambaram AI.A. 2012. Growth and yield parameters using gamma rays in bhindi [Abelmoschus esculentus (L.) Moench]. var. arkaanamika. International Journal of research in Plant Science 2(4), 56-58.
Khan S, Goyal S. 2009. Improvement of mung bean varieties through induced mutations. African Journal of Plant Science 3, 174-180.
Kozgar MI, Goyal S, Khan S. 2011. EMS induced mutational variability in Vigna radiate (L) and Vigna mungo (L). Research Journal of Botany 6, 31-37.
Kumar S, Parekh MJ, Fougat RS, Patel SK, Patel CB, Kumar M. 2017. Assessment of genetic diversity among okra genotypes using SSR markers. Journal of Plant Biochemistry and Biotechnology 26, 172-178.
Laemmli UK. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
Lee YI, Lee IS, Lim YP. 2002. Variation in sweet potato regenerates from gamma-rays irradiated embryogenic callus. Journal of Plant Biotechnology 4(4), 163-170.
Lowry OH, Rosebrough AL, Farr and Randall RJ. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
Manivel P, Prabakaran V, Parvathi S, Palanivel S. 2022. Effect of gamma rays on growth and GC-MS analysis in bhindi [Abelmoschus esculentus (L.) Moench]. Indian Journal of Natural Sciences 13(74), 49195-49201.
Melki M, Marouani A. 2010. Effects of gamma rays irradiation on seed germination and growth of hard wheat. Environmental Chemistry Letters 8, 307-310.
Mohamed IFF. 2000. Characterization of some local varieties of Cucurbitaceae growing under Egytian conditions. M. Sc. Thesis, Faculty of Agriculture, Ain-Shams University.
Mostafa GG. 2011. Effect of sodium azide on the growth and variability induction in (Helianthus annuus L.) International Journal of Plant Breeding and Genetics 5(1), 76-85.
NRCNAs. 2006. “Okra”. Lost Crops of Africa: vol II: Vegetables. Lost Crops of Africa.2.Washington, D.C: National Academies Press.
Oppong-Sekyere D, Akromah R, Nyamah EY, Brenya E, Yeboah S. 2011. Characterization of okra (Abelmoschus spp. L.) germplasm based on morphological characters in Ghana. Journal of Plant Breeding and Crop Science 3(13), 367-378.
Patil P, Sutar S, Joseph JK, Malik S, Rao S, Yadav S. 2015. A systematic review of the genus Abelmoschus (Malvaceae). Rheedea 25(1), 14-30.
Pavadai P, Girija M, Dhanavel D. 2010. Effect of gamma rays on some yield parameters and protein content of soybean in M2, M3 and M4 generation. Journal of Experimental Science 1(6), 8-11.
Ramasamymm. 1973. Investigation on induced mutagenesis in Blackgram Ph.D. Thesis, Tamil Nadu Agriculture University. Coimbatore.
Rashed MA, Fahmy, EM, Sallam MA. 1994. Embryo culture, protein and isoenzyme electrophoresis as selectable marker to predict salt tolerance in wheat. 5th Conf. Agricultural Development Research Faculty of agriculture, Ain Shams Uni. Cario, Egypt 1, 469-490.
Sathish Kumar D, Eswar Tony D, Praveen Kumar A, Ashok Kumar K, Bramha Srinivasa Rao D, Ramarao N. 2013. A review on: Abelmoschus esculentus (Okra). International Research Journal of Pharmaceutical and Applied Sciences 3(4), 129-132.
Tah PR. 2006. Induced macro mutation in mungbean (Vigna radiate (L.) Wilczek). International Journal of Botany 2, 219-228.
P. Manivel, V. Prabakaran, S. Parvathi, S. Palanivel, 2022. Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]. J. Biodiv. Environ. Sci., 21(5), 268-274.
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