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Research Paper | August 1, 2018

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Effect of green synthesized copper nanoparticles on seed germination and seedling growth in wheat

Sidra Sabir, Muhammad Arshad, Saboon, Seema Hassan Satti, Tayyaba Batool, Farah Farqaulit, Sunbal Khalil Chaudhari

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Int. J. Biosci.13(2), 28-35, August 2018

DOI: http://dx.doi.org/10.12692/ijb/13.2.28-35


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The green synthesis and application of metallic nanoparticles has become a significantly important branch of nanotechnology. In present study, we report the green synthesis of Copper nanoparticles using leaf extract of Moringa oliefera as reducing agent and tested their effects on seed germination as well as seedling growth. The exposure of copper to leaf extract resulted in rapid reduction of copper ions leading to the formation of copper NPs in the solution. The UV visible spectroscopy and X-ray Diffraction analysis confirmed the synthesis of Cu NPs. Biologically synthesized Cu NPs were applied to evaluate the effect on seed germination and seedling growth of Triticum aestivum, an economically important food crop. Seeds were treated with different concentrations of Cu NPs ( 25ppm, 50ppm, 75ppm and 100ppm) and tested against control. Higher percentage of seed germination was observed in seeds treated with low concentrations of Cu NPs. Also, a significant increase in root fresh weight, root dry weight, root length, and root elongation was recorded at lower concentrations of Cu NPs as compared to the control. However, the treatment with Cu NPs above 75ppm showed delayed germination and overall inhibitory effect on seedling growth. The results of this experiment revealed that the application of Cu NPs enhanced the germination in wheat.


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Effect of green synthesized copper nanoparticles on seed germination and seedling growth in wheat

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