Effect of green synthesized copper nanoparticles on seed germination and seedling growth in wheat
Paper Details
Effect of green synthesized copper nanoparticles on seed germination and seedling growth in wheat
Abstract
The green synthesis and application of metallic nanoparticles has become a significantly important branch of nanotechnology. In present study, we report the green synthesis of Copper nanoparticles using leaf extract of Moringa oliefera as reducing agent and tested their effects on seed germination as well as seedling growth. The exposure of copper to leaf extract resulted in rapid reduction of copper ions leading to the formation of copper NPs in the solution. The UV visible spectroscopy and X-ray Diffraction analysis confirmed the synthesis of Cu NPs. Biologically synthesized Cu NPs were applied to evaluate the effect on seed germination and seedling growth of Triticum aestivum, an economically important food crop. Seeds were treated with different concentrations of Cu NPs ( 25ppm, 50ppm, 75ppm and 100ppm) and tested against control. Higher percentage of seed germination was observed in seeds treated with low concentrations of Cu NPs. Also, a significant increase in root fresh weight, root dry weight, root length, and root elongation was recorded at lower concentrations of Cu NPs as compared to the control. However, the treatment with Cu NPs above 75ppm showed delayed germination and overall inhibitory effect on seedling growth. The results of this experiment revealed that the application of Cu NPs enhanced the germination in wheat.
Abdul-Baki AA, Anderson JD. 1973. Vigor determination in soybean and seed multiple criteria. Crop Sciences 13, 630–633. http://dx.doi.org/10.2135/cropsci1973.0011183X001300060013x.
Asseng S, Foster I, Turner NC. 2011. The impact of temperature variability on wheat yields. Global Change in Biology 17, 997–1012. https://doi.org/10.1111/j.1365-2486.2010.02262.x
Banerjee P, Satapathy M, Mukhopahayay A, Das P. 2014. Leaf extract mediated green synthesis of silver nanoparticle from widely available Indian plants. Synthesis, characterization, antimicrobial property and toxicity analysis. Bioresources and Bioprocessing 1-10. https://doi.org/10.1186/s40643-014-0003-y.
Iqbal M, Asif S, Ilyas N, Raja NI, Hussain M, Shabir S, Faz MNA, Rauf A. 2016. Effect of plant derived smoke on germination and post germination expression of wheat (Triticum aestivum L.). American Journal of Plant Sciences 7, 806–813. http://dx.doi.org/10.4236/ajps.2016.76075
Khodakovskaya MV, Silva KD, Biris AS, Dervishi E, Villagarcia H. 2012. Carbon nanotubes induce growth enhancement of tobacco cells. American Chemical Society Nano 6, 2128–2135.
Krishnaraj C, Jagan EG, Ramachandran R, Abirami SM, Mohan N, Kalaichelvan PT. 2012. Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism. Process Biochemistry 4, 651–658. https://doi.org/10.1016/j.procbio.2012.01.006
Mittal AK, Kaler A, Banerjee UC. 2013. Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from flower extract of Rhododendron dauricum. Biotechnology Advances 31, 346-356.
Nel A, Xia T, Madler L, Li N. 2006. Toxic potential of materials at the nano level. Science 311, 622-627. https://doi.org/10.1126/science.1114397
Ngo QB, Dao TH, Nguyen HC, Tran XT, Nguyen TV, Khuu TD, Huynh TH. 2014. Effects of nanocrystalline powders (Fe, Co and Cu) on the germination, growth, crop yield and product quality of soybean (Vietnamese species DT-51). Advances in Natural Sciences: Nanoscience and Nanotechnology 5, 15–23.
Salama HMH. 2012. Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). International Research Journal of Biotechnology 3, 190–197.
Sharma P, Jha AB, Dubey RS, Pessarakli M. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 12, 134–136.
Sidra Sabir, Muhammad Arshad, Saboon, Seema Hassan Satti, Tayyaba Batool, Farah Farqaulit, Sunbal Khalil Chaudhari, 2018. Effect of green synthesized copper nanoparticles on seed germination and seedling growth in wheat. Int. J. Biosci., 13(2), 28-35.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.