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Effect of growth regulators on rooting of Andrographis neesiana Wight. – a valuable endemic medicinal plant of India

Chinnappan Alagesaboopathi

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Int. J. Biosci.3(1), 49-57, January 2013


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The present study deals with the vegetative propagation prospects of an endemic medicinal plant Andrographis neesiana Wight, which is familiarly used in herbal medicine for the treatment of several diseases. Due to over exploitation this plant is disappearing from original habitat hence its cultivation on commercial scale is suggested. Stem cuttings of Andrographis neesiana are advantageous to root. Treatment with Indole-3-Butyric Acid (IBA) and a-Napthalene Acetic Acid (NAA) raised rooting and increased shoot development in greenhouse under intermittent misting. IBA and NAA treated cuttings performed improve in all development parameters compared to control. The highest percentage of rooting was observed in IBA 1500 ppm (68.10%). The maximum root length was noted in IBA 2000 ppm (11.20 cm). The roots were profuse and branched in characteristic. Besides this, the survival rate of IBA treated plantlets was 86.45% and NAA treated plantlets was 72.61% in comparison to control where it was 7.32%. The percentage of rooting and root length upgraded by using plant growth hormones, either separate or together. The present investigation concludes that clonal multiplication of these an endemic medicinal plant is feasible through application of plant growth regulators. The significance of these findings on the propagation and conservation of A. neesiana is discussed.


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Effect of growth regulators on rooting of Andrographis neesiana Wight. – a valuable endemic medicinal plant of India

Ahmedullah M, Nayar MP. 1986. Endemic plants of the Indian Region. Botanical Survey of India, Calcutta. 1,143-146.

Akwatulira F, Gwali S, Ssegawa P, Okullo, JBL, Tumutebaze SB, Mbwambo JR, Muchugi A. 2011. Vegetative propagation of Warburgia ugandensis Sprague: An important medicinal tree species in eastern African Journal of Medicinal Plants Research. 5 (30), 6615-6621.

Alagesaboopathi C . 2011. Use of auxins in vegetative propagation of Andrographis lineata Nees. An endemic medicinal plant from Southern India. Middle –East Journal of Scientific Research. 10(4), 450-454.

Alagesaboopathi C, Balu S. 2000. Antifungal activity of some species of Andrographis Wallich Ex Nees on Helminthosporium oryze Breda dehaan. Journal of Economhic and Taxonomic Botany. 24,705-707.

Alagesaboopathi C, Balu. S. 1999. Ethnobotany of Indian Andrographis Wallich Ex. Nees. Journal of Economic and Taxonomic Botany. 23,29-32.

Alagesaboopathi C, Sivakumar R. 2011. Antimicrobial properties of various extracts of Andrographis neesiana Wight. An endemic medicinal species from India. International Journal of Pharm Tech Research. 3(1), 27-31.

Anonymous. 1948. Wealth of India – Raw Materials, Vol. I, CSIR, New Delhi. p. 76-78.

Anyasi RO. 2011. The effects of Indole Butyric Acid. IBA on rooting of Chromolaena odorata. International Journal of Medicinal and Aromatic Plants. 1(3), 212-218.

Baul  TK,  Mezbahuddin  M,  Mohiuddin  M. 2008. Vegetative propagation and initial growth performance of Stereospermum suaveolens DC: A wild tropical tree species of medicinal value. New Forests. 37(3), 275-283.

Che Man NB. 2010. Phytochemical analysis of the leaves of Chromolaena odorata. Asteraceae. B.Sc. Hons. Thesis, faculty of Applied Sciences University of Technology, Mara, 9-12.

Chinnappan Alagesaboopathi. 2012. Influence of Indole acetic acid and Indole butyric acid on root development and status of Andrographis elongata . Vahl T. And. – An endemic medicinal plant of India. International Journal of Biosciences. 2(4), 75-81.

Dhillion RS, Hooda MS, Pundeer JS, Ahlawat KS, Kumari S. 2009. Development of efficient techniques for clonal multiplication of Jatropha curcus L. a potential biodiesel plant. Current Science. 96(6), 823-827.

Fielding JM. 1969. Factors affecting rooting and growth of Pinus radiata cuttings in the open nursery. Bulletin of Commonwealth Australian Forestry and Timber Buteau. 45, 36.

Gaikwad RS. 2011. Vegetative propagation of Jatropha species by stem cuttings. Current Botany. 2(1), 39-40.

Gamble JS. 1982. Flora of the Presidency of Madras Vol.II. Botanical Surveys of India. Calcutta. p. 1045-1051.

Hartman TH, Kester ED, Davies FTJr. 1990. Plant propagation principles and practices. Fifth Edition. Simon and Schuster, Engle wood cliffs, New Jersey, U.S.A.

Henry AN. Kumari GR, Chitra V. 1987. Flora of Tamilnadu, India, Series 1: Analysis Vol.I. Botanical Survey of India. Southern Circle, Coimbatore. p, 138-141.

Hobbie L, McGovern M, Hurvitz LR, Pierro A, Liu NR, Bandyopadhyay A, Estelle M. 2000. The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development. 127, 22-32.

Hopkins WG. 1999. Introduction to plant physiology, John Wiley and Sons.

Kesava Reddy M, Vijaya Bhaskar Reddy M, Anil Kumar Reddy B, Gunasekar D, Caux, C, Bodo B. 2003. A new chalcone and a flavone from Andrographis neesiana. Chemical and Pharmaceutical Bulletin. 51(7), 854-856.

Kirtikar KR, Basu BD. 1975. Indian medicinal plants, Bishen Singh Mahendrapal Singh, New Delhi. 3, 1884-1886.

Majeed M, Khan MA, Mughal AH. 2009. Vegetative propagation of Aesulus indica through stem cuttings treated with plant growth regulators. Journal of Forestry Research. 20(2), 171-173.

Nambison KMP, Subhaiah R, Rajesekaran K, Manivel L. 1977. Effect of age of wood time and growth regulators on rooting of cuttings of Nerium olender. South Indian Horticulture. 25(4), 151-153.

Nanda KK. 1970. Investigation on the use of auxin in vegetative reproduction of forest plants. Final Report PL 480. Research Project, p. 215.

Ooyamma N, Toyoshima A. 1965. Rooting ability of pine cuttings and its promotion. Bulletin of Governmental Forestry Experimentation Station. 179, 99-125.

Ponvinobala K, Kanchana G, Rubalakshmi G. 2012a. In vitro anticancer activity of hydro-alcohol extract of leaves of Andrographis neesiana against PC-3 and MCF-7 cell lines. International Journal of Pharmacy and Pharmaceutical Sciences. 4(3), 396-399.

Ponvinobala K, Kanchana G, Rubgalakshmi G. 2012b. In vitro antioxidant activity of hydroalcoholic extract of Andrographis neesiana leaves. Journal of Pharmacy Research. 5(2), 1256-1259.

Saradha M, Paulsamy S. 2012. Effect of growth hormones on rooting attributes of stem cuttings of endangered plant species, Hildegardia populifolia . Roxb. Schott and Endl. Sterculiaceae. International Journal of Biology, Pharmacy and Allied Science. 1(8), 1145-1152.

Senthilkumar  P,  Paulsamy  S,  Vijayakumar KK. 2009. Rooting ability of Rubia cordifolia L. A. potential therapeutic plant species in the Western Ghats, Nilgiri biosphere reserve, India. Scientific Transactions in Environment and Technovation. 2. 4, 187-190.

Shamet GS, Khosla PK, Surinder K. 1989. Preliminary study on rooting of Celtis australis and Punica granatum. Indian Journal of Forestry. 12. 4, 321-322.

Sharma LK, Pandey ON. 1999. Effect of plant growth regulators in rooting behaviour of cuttings of Dalbergia latifolia Roxb. and Dalbergia sisso Roxb. Indian Forester. 125(4), 421-426.

Sivarajan V, Balachandran I. 2001. Ayurvedic drugs and their plant sources, Oxford and IBH Publishing, New Delhi. 243-245.

Sofowora A. 1993. Medicinal plant and traditional medicine in Africa. John Wiley and Sons Ltd. 150-153.

Thakur IK, Gupta R. 1998. Effect of auxins on rooting of Alnus nitida Endl. Cuttings. Indian Journal of Forestry. 21. 2,174-175.

Tiwari RKS, Kuntal Das. 2010. Effect of stem cuttings and hormonal pre-treatment of propagation of Embella tsierian and Caejalpina bonduc, two important medicinal plant species. Journal of Medicinal Plants Research 4(15), 1577-1583.

Torkashvand AM, Shadparvar V. 2012. Rooting in Hibiscus rosa – sinensis (Yellow Double Hybrid) By  Indole  Butyric  Acid  and  rooting  substrates. International   Journal   of   Plant,   Animal   and Environmental Sciences 2(2), 194-197.

Vinayakumar  J,  Shirol  AM,  Kulkarni  BS, Krishnamurthy GH, Reddy BS. 2008. Effect of growth   regulators   on   rooting   of   Thunbergia grandiflora.   Karnataka   Journal   of   Agriculture Science. 21(2), 322-323.


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