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Effect of magnetic and electromagnetic field on spore germination and ligninolytic activities of Ganoderma applanatum using solid state fermentation

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Research Paper 01/07/2019
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Effect of magnetic and electromagnetic field on spore germination and ligninolytic activities of Ganoderma applanatum using solid state fermentation

Mohamed Yasser Bedaiwy
Int. J. Biosci.15( 1), 475-486, July 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Basidiospores germinationLigninolytic activityMagnetic and electromagnetic fieldSolid state fermentation

Abstract

There are many attempts to increase the germination rate of basidiospores using temperature, light or chemicals. The current study has been designed to use the magnetic and electromagnetic fields to stimulate the germination of basidiospores from Ganoderma applanatum. Mature, healthy and fresh basidiocarpe of Ganoderma applanatum (Aphyllophorales: hymenochaetaceae), causes white rot of hard wood was isolated from Alsantah city (Delta Nile region of Egypt) in autumn 2018 from Casuarina tree. Basidiospores were collected from the fruiting body and cultivated on different microbial growing media. Basidiospores were obtained and exposed to magnetic field (MF) and electromagnetic field (EMF) for different times of exposure extended for one hour. Basidiospores germination was stimulated when exposed to both fields. The electromagnetic field was more stimulating than the magnetic field in basidiospores germination where the spore’s germination rate reached 18.7% after exposure to the electromagnetic field for 40 min while the germination rate was 14.6% after exposure to magnetic field for 50 min. The fruiting body of the fungus was cultured to obtain fungal mycelium, and the resulting mycelium was exposed to the magnetic and electromagnetic field for different exposure times. The resulting mycelia was used to inoculate five different lignocellulosic substrate (Rice straw, Wheat straw, Wheat bran, Rice bran and Sawdust) using solid state fermentation in order to test its efficiency in these wastes degradation. Furthermore, Lignin degrading enzymes including (Laccase, Manganese-dependent peroxidases and Lignin peroxidase) were measured. The three tested enzymes showed a marked increase in their activity after exposure to the magnetic field for 20 minutes (laccase 17.8-unit, lignin peroxidase 11.1-unit and Mn dependent peroxidase 13.5- unit). Concerning the effect of electromagnetic field the activity of lignin degrading enzymes were enhanced after exposure to the electromagnetic field for 20 minutes (laccase 19.5- unit, lignin peroxidase 15.1-unit and Mn dependent peroxidase 27.4-unit). Therefore, the obtained data suggest that the using of both magnetic and electromagnetic fields could be used to break down the dormancy state of the basidiospores in order to increase the rate of spores germination and at the same time increase the activity of lignin degrading enzymes.

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