Effect of magnetic and electromagnetic field on spore germination and ligninolytic activities of Ganoderma applanatum using solid state fermentation

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Research Paper 01/07/2019
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Effect of magnetic and electromagnetic field on spore germination and ligninolytic activities of Ganoderma applanatum using solid state fermentation

Abstract

There are many attempts to increase the germination rate of basidiospores using temperature, light or chemicals. The current study has been designed to use the magnetic and electromagnetic fields to stimulate the germination of basidiospores from Ganoderma applanatum. Mature, healthy and fresh basidiocarpe of Ganoderma applanatum (Aphyllophorales: hymenochaetaceae), causes white rot of hard wood was isolated from Alsantah city (Delta Nile region of Egypt) in autumn 2018 from Casuarina tree. Basidiospores were collected from the fruiting body and cultivated on different microbial growing media. Basidiospores were obtained and exposed to magnetic field (MF) and electromagnetic field (EMF) for different times of exposure extended for one hour. Basidiospores germination was stimulated when exposed to both fields. The electromagnetic field was more stimulating than the magnetic field in basidiospores germination where the spore’s germination rate reached 18.7% after exposure to the electromagnetic field for 40 min while the germination rate was 14.6% after exposure to magnetic field for 50 min. The fruiting body of the fungus was cultured to obtain fungal mycelium, and the resulting mycelium was exposed to the magnetic and electromagnetic field for different exposure times. The resulting mycelia was used to inoculate five different lignocellulosic substrate (Rice straw, Wheat straw, Wheat bran, Rice bran and Sawdust) using solid state fermentation in order to test its efficiency in these wastes degradation. Furthermore, Lignin degrading enzymes including (Laccase, Manganese-dependent peroxidases and Lignin peroxidase) were measured. The three tested enzymes showed a marked increase in their activity after exposure to the magnetic field for 20 minutes (laccase 17.8-unit, lignin peroxidase 11.1-unit and Mn dependent peroxidase 13.5- unit). Concerning the effect of electromagnetic field the activity of lignin degrading enzymes were enhanced after exposure to the electromagnetic field for 20 minutes (laccase 19.5- unit, lignin peroxidase 15.1-unit and Mn dependent peroxidase 27.4-unit). Therefore, the obtained data suggest that the using of both magnetic and electromagnetic fields could be used to break down the dormancy state of the basidiospores in order to increase the rate of spores germination and at the same time increase the activity of lignin degrading enzymes.

VIEWS 19

Abdel-Hamid AM, Solbiati JO, Cann IKO. 2013. Insights into lignin degradation and its potential industrial applications. Advances in Applied Microbiology 82, 1-28.

Bugg TDH, Rahmanpour R. 2015. Enzymatic conversion of lignin into renewable chemicals. Current Opinion in Chemical Biology 29, 10-17. PMID: 26121945 https://doi.org/10.1016/j.cbpa.2015.06.009

Bulseco MG, Abella E, Reyes RG. 2005. Morphogenesis of Schizophyllum commune, a wild edible mushroom of Mt. Nagpale, Abucay, Bataan, Philippines. Journal of Nature Studies 4(1), 20–28.

Butler MJ, Day AW. 1998. Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi. International Journal of Plant Sciences 159(6), 989-995.

D’enfert C. 1997. Fungal spore germination: Insights from the molecular genetics of Aspergillus nidulans and Neurospora crassa. Fungal Genetics and Biology 21(2), 163–172. http://dx.doi.org/10.1104/pp.47.5.729

Feofilova EP, Tereshina VM, Garibova LV, zavyalova LA, Memorsky AS, Maryshova NS. 2004. Basidiospores of Agaricus bisporus germination. Applied Biochemistry and Microbiology 40(2), 220-226.

Feofilova EP, Ivashechkin AA, Alekhin AI, Sergeeva Ia É. 2012. Fungal Spores: Dormancy, Germination, Chemical Composition, and Role in Biotechnology (Review). Prikladnaia Biokhimiia Mikrobiologiia 48(1), 5-17

Florez M, Carbonell MV, Martinez E. 2007. Exposure of maize seeds to stationary magnetic fields: effects on germination and early growth. Environmental and Experimental Botany 59, 68-75.

Forsythe A, Vogan A, Xua J. 2016. Genetic and environmental influences on the germination of basidiospores in the Cryptococcus neoformans species complex Scientific Report 6, 33828. http://dx.doi.org/10.1038/srep33828

Fries N. 1984. Spore germination in the higher Basidiomycetes. Proceedings of the Indian Academy of Sciences. (Plant Science.) 93(3), 205-222.

Fries N. 1987. Ecological and evolutionary aspects of spore germination in the higher basidiomycetes. Transaction of the British Mycological Society 88, 1– 7. http://dx.doi.org/10.1016/S0007-1536(87)80179-1

Giovannozzi-Sermanni G, Cappelletto P, Stazi S, Perani C, Porri A, Falesiedi G. 2001. Paper pulps from agricultural residues by a biotechnological process. http://www.unitus.it/lignocellulose/lignocellulose.htm

Gonzalo G, de Colpa DI, Habib MHM, Fraaije MW.2016. Bacterial enzymes involved in lignin degradation. Journal of Biotechnology 236, 110-119.

Hammel KE, Cullen D. 2008. Role of fungal peroxidases in biological ligninolysis. Current opinion in plant biology 11, 349–355.

Hatakka A. 1994. Lignin-modifying enzymes from selected white-rot fungi – production and role in lignin degradation. FEMS Microbiology Review 13, 125–135.

Hatakka A, Hammel KE. 2010. Fungal biodegradation of lignocellulose. In Hofrichter M (ed.): Industrial Application (2nd Ed.) The Mycota, Vol. X. Springer: Berlin, p 319–340.

Hilton RN. 1961. Sporulation of Fomes lignosus, Fomes noxius and Ganoderma pseudoferreum. Proceedings of the Natural Rubber Research Conference, Kuala Lumpur 1960, p 496–502.

Iqbal M, Haq Z, Jamil Y, Ahmad MR. 2012. Effect of presowing magnetic treatment on properties of pea. International Agrophysics 26, 25-31.

Jamil Y, Haq Z, Iqbal M, Perveen T, Amin N. 2012. Enhancement in growth and yield of mushroom using magnetic field treatment. International Agrophysics 26, 375-380. http://dx.doi.org/10.2478/v10247-012-0052-4

Jaqueline M, Mendonça Maciel M, Silva AC, Ribeiro HC. 2010.  Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota. Electronic Journal of Biotechnology, 13(6). http://dx.doi.org/10.2225/vol13-issue6-fulltext-2

Kadowaki K, Leschen RAB, Beggs JR. 2010. Periodicity of spore release from individual Ganoderma fruiting bodies in a natural forest. Australian Mycology 29, 17–23.

Kalmis E, Kalyoncu F. 2006. Variations in the isolates obtained from basidiospores of commercial mushroom Lentinula edodes (Shiitake). International Journal of Science & Technology 1(2), 99-103.

Kuhara F, Papinuttib L. 2014. Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers. Revista Argenti na D E Microbiología 46(2), 144-149

Leonowicz A, Matuszewska A, Luterek J. 1999. Biodegradation of lignin by white rot fungi. Fungal Genetics Biology 27(2-3), 175-185.

Lim TM. 1977. Production, germination and dispersal of basidiospores of Ganoderma pseudoferreum on Hevea. Journal of Rubber Research Institute of Malaysia 25, 93–99.

Marks N, Szecówka PS. 2010. Impact of variable magnetic field stimulation on growth of aboveground parts of potato plants. International Agrophysics 24, 165-170.

Mills CFL, Eilers FI. 1973. Factors Influencing the Germination of Basidiospores of Coprinus radiates. Journal of General Microbiology 77, 393-401.

Min BS, Nakamura N, Miyashiro H, Bae KW, Hattori M. 1998. Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chemical and Pharmaceutical Bulletin 46(10), 1607–1612. http://dx.doi.org/10.1248/cpb.46.1607

Moore D,  Robson GD, Trinci APJ. 2011. 21st Century Guidebook to Fungi (Kindle Edition) United Kingdom, Cambridge university press.

Nagy P, Fisch G. 2002. “Effect of UV and visible light irradiation on mycelial growth and sclerotium formation of Sclerotinia sclerotium”, Acta Ohytopathologica et Endemiological Hungarica 37, 83-89.

Owen RD. 1998. “MYC mRNA abundance is unchanged in subcultures of HL60 cells exposed to power line frequency magnetic fields”, Radiation Research 150, 23-30.

Page DE, Glen M, Ratkowsky DA, Beadle CL, Rimbawanto A, Mohammed CL. 2017. Ganoderma basidiospore germination responses as affected by spore density, temperature and nutrient media. Tropical Plant Pathology 42(5), 328-338.

Pandey NK, Maravi KK, Kerketta A, Taram L. 2016. Asimple technique for isolation of single basidiospore from Pleurotus flabellatus. Research Journal of Agriculture science 7(3), 703-704.

Pei-Yu H, Jiun-Liang CH, Hsing-Yu CH, Sien-Hung Y. 2012. Extract of sporoderm-broken germinating spores of Ganoderma lucidum activates human polymorphonuclear Neutrophils via the P38 mitogenactivated protein kinase pathway. Chang Gung Medical Journal 35(2), 140-148.

Poyedinok N, Negriyko A, Mikhailova O. 2015. Effects of light wavelengths and coherence on basidiospores germination.  Journal of Microbiology, Biotechnology and Food Science 4(4), 352-357.

Ruzic R, Gogala N, Jerman L. 1997. “Sinusoidal magnetic fields: Effects on growth and ergosterol content in mycorrhizal fungi”, Electro and Magneto Biology 16, 129-142.

Sigoillot JC, Berrin JG, Bey M. 2012.Fungal strategies for lignin degradation. In: Jouanin L, Lapierre C (eds.) Lignins: Biosynthesis, Biodegradation and Bioengineering vol. 61, London, UK: Academic Press, Elsevier, p 263–308.

Sugano Y. 2009. DyP-type peroxidases comprise a novel heme peroxidase family. Cellular and Molecular Life Sciences 66(8), 1387-1403.

Vashisth A, Nagarajan S. 2010. Effect on germination and early growth characteristic in sunflower seeds exposed to static magnetic field. Journal of Plant Physiology 167, 149-156.

Wasak A, Drozd R, Jankowiak D, Rakoczy R. 2019. Rotating magnetic field as tool for enhancing enzymes properties – laccase case study. Science report 9, 3707. http://dx.doi.org/10.1038/s41598-019-39198-y

Xin L, jian-ping Y, Xiao-Jun CH. 2002. Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum. Cancer Letters 182(2), 155–161. http://dx.doi.org/10.1016/S0304-3835(02)00080-0

Zhu HS, Yang XL, Wang LB, Zhao DX, Chen L. 2000. Effects of extracts from sporoderm-broken spores of Ganoderma lucidum on HeLa cells. Cell Biology and Toxicology 16(3), 201–206. http://dx.doi.org/10.1023/A:1007663006548

Anggoro B, Pakpahan PM, Kusnoaji FD, Sirait KT. 1999. Influence of 50 hz magnetic field on growth of mushroom species: shitake (lentinus edodes) and oyster (pleurotus astreatus). High Voltage Engineering Symposium, 22-27 August 1999 Conference Publication No. 467, 1.359.P6.

Adey WR. 1992. Frequency and power windowing in tissue interaction with weak electromagnetic fields. Proceedings of the Institute of Electrical and Electronics Engineers (IEEE) 68(1), 119-125.