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Effect of media amendments on invivo root and shoot organogenesis of stevia (Stevia rebaudiana L.)

Research Paper | April 1, 2019

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Abdul Basit, Inayat Ullah, Syed Tanveer Shah, Izhar Ullah, Noor Alam, Syed Abdul Qadir Gilani

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Int. J. Biosci.14( 4), 55-63, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.55-63


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Stevia, also known as sugar leaf or sweet weed, a highly valuable and medicinal plant domesticated recently in many countries of the world. Propagation of stevia usually by stem cuttings root easily, but require high labor inputs. Poor seed germination is one of the factors limiting large-scale cultivation. Therefore, an experimental study was carried out with objective to evaluate performance of nodal cutting of stevia under different media amendments (To = Garden Soil, T1 = IBA + Soil, T2 = FYM + soil (1:1), T3 = Leaf mold + soil (1:1). Randomized Complete Block Design (RCBD) with factorial arrangements having 4 treatments replicated 4 times were used during experimentation. Data analysis showed that highest value of survival percentage (91.75 %), plant height (36.18cm), number of branches (3.00), number of leaves (42.50), stem diameter (5.38mm), number of inflorescence (9.50), root length (10.00cm), fresh root weight (5.23g) and dry root weight (0.96g)  were recorded in treatment T1 (IBA+Soil). Whereas maximum number of days to spike emergence were taken by T0 (47.00). It was concluded from the research findings that indole butyric acid (IBA) of 150ppm could be used as an alternative for enhancing the growth attributes of stevia under the agro-climatic conditions of National Agricultural Research Centre Islamabad.


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Effect of media amendments on invivo root and shoot organogenesis of stevia (Stevia rebaudiana L.)

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