Paper Details

Effect of melatonin on in vitro maturation of bovine oocyte

Beheshti

DOI: https://dx.doi.org/10.12692/ijb/4.10.31-35

Int. J. Biosci. 4(10), 31-35. May, 2014. (PDF)

Abstract:

This study was aimed at determining the effect of melatonin supplement during in vitro maturation of bovine oocytes. Ovaries were obtained from local abattoir shortly after slaughter and transported to the laboratory in phosphate buffer solution plus 100 IU/ml potassium penicillin G and 100 μg/ml streptomycin sulfates at 35 °C, within 2 hours from slaughter. Cumulus-oocyte complexes (COCs) from abattoir ovaries were collected in were matured in vitro in Hepes- TCM199 supplemented 0 (control), 50 or 100 μmol/ml of melatonin for 24 hours. When COCs matured in TCM 199 media with 50 μmol/ml melatonin, the rate of maturation were increased none significantly as compared with control group (P>0.05). The expansion rate of cumulus oopherus in TCM199 with 50 and 100 μmol/ml melatonin were not increased significantly. Also, the rates of degenerated oocytes in treatment groups lower than control group that it is not significant. In conclusion, melatonin can not increased the bovine oocyte maturation in the TCM199 medium.