Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae)
Paper Details
Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae)
Abstract
Insect growth regulators (IGRs) belong to a class of compounds which interfere with normal growth, development and reproduction of insects. Through greater selectivity of action IGRs have less undesirable effects on man, wild life and environment. Many of the IGRs mimic the action of insect hormones, ecdysone or juvenile hormone (JH). Methoxyfenozide is a potent non-steroidal ecdysone agonist developed as an insecticide and is effective against lepidopteran pests. The effects of methoxyfenozide (RH-2485) on reproduction of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), an important pest in stored products worldwide, were evaluated under laboratory conditions. Treatment at LD50 (0.01 μg/pupa) and LD90 (0.37 μg/pupa) was made by topical applications on newly ecdysed female pupae. Data showed that methoxyfenozide, significantly affected reproductive parameters in the ovaries in treated females such as amounts of proteins, vitellogenin and vitellin as compared to the control series. In addition, electrophoretic separation of ovarian proteins by sodium dodecyl sulfate polyacrylamide slab gels (SDS-PAGE) showed that the treatment with the ecdysteroid agonist resulted in reduction in the number and the intensity of some proteins bands compared to controls. The major proteins in the ovaries of lepidopteran insects are members of the storage protein family and as storage proteins are crucial for insect development they may be targeted for developing better insect control strategies.
Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principe of protein-dye binding. Analytical Biochemistry (72), 248-254.
Dhadialla TS, Retnakaran A, Smagghe G. 2005.Insect growth and development disrupting insecticides. In: Gilbert, L.I., Kostas, I. and Gill, S. (eds.) Comprehensive Insect Molecular Science. Pergamon Press, New York, NY (6), 55-116.
Engelmann F. 1979. Insect vilellogenin: identification, biosynthesis and role in vitellogenesis. In: Treherne J E., Berridge M J., Wigglesworth V B. (Eds). Advances in Insect Physiology, New York, Academic Press, 49-108.
Fabre MC, Descamps M, Baert JL. 1992. Identification and partial characterization of vitellin and vitellogenin from Scolopendra cingulata Latreille (Myriapoda Chilopoda). In: Meyer, E., Thaler, K. & Schedl, W. (Eds) Advances in Myriapodology 117-121.
Kanost M, Kawooya JK, Lan RO, Van Heudsden MC, Ziegler R. 1990. Insect hemolymph proteins. Advances In Insect Physiology (22), 299-396.
Laemmli VK. 1970. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature (227), 680-685.
Lafont R, Dauphin-Villemant C, Warren JT, Rees HH. 2005. Ecdysteroid chemistry and biochemistry. In: Gilbert LI, Iatrou K, Gill SS, editors. Comprehensive Molecular Insect Science.Oxford: Elsevier; 125-95 p.
Meskache R, Soltani-Mazouni N. 2013. Activité comparée de quatre agonistes de l’hormone de mue chez Ephestia kuehniella: composition biochimique des testicules et potentiel reproducteur. Bulletin de la Société Zoologique de France 138 (1-4), 177-187.
Postlethwait JH, Bownes M, Jowett T. 1980. Sexual phenotype and vitellogenin synthesis in Drosophila melanogaster. Developmental Biology 79(2), 379-387.
Rajathi A, Pandiarajan J, Krishnan M. 2010. Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori. Biologia (65), 903-913. https://doi.org/10.2478/s11756-010-0104-9.
Resmitha C, Reshma RM, Bindu P, Kannan VM. 2014. The ecdysone mimic, methoxyfenozide, alters the level of major haemolymph proteins in the larvae of Spodoptera mauritia Boisd (Lepidoptera: Noctuidae). ActaBiologicaIndica3(2), 726-730.
Riddiford LM. 1994. Cellular and molecular actions of juvenile hormone I. In: PD Evans. Advances in Insect Physiology. Academic Press. pp. 213-274.
Rodríguez–Enríquez CL, Pineda S, Figueroa JI, Schneider MI, Martínez AM. 2010. Toxicity and sublethal effects of methoxyfenozide on Spodoptera exigua (Hũbner) (Lepidoptera: Noctuidae). Journal of Economic Entomology 103(3), 662-667. https://doi.org/10.1603/EC09244.
Sadeghi A, Van Damme EJM Smagghe G. 2009. Evaluation of the susceptibility of the pea aphid, Acyrthosiphon pisum, to a selection of novel biorational insecticides using an artificial diet. Journal of Insect Science volume 9 (65), 522-529. https://doi.org/10.1673/031.009.6501
Sappington TW, Raikhel AS.1998. Molecular characteristics of insect vitellogenin and vitellogenin receptor. Insect Biochemistry and Insect Molecular Biology (28), 277-300. https://doi.org/10.1016/S0965-1748(97)00110-0.
Soltani-Mazouni N, Hami M, Gramdi H. 2012.Sublethal effects of methoxyfenozide on reproduction of the Mediterranean flour moth, Ephestia Kuehniella Zeller. Invertebrate Reproduction and Development, 56 (2), 157-163. https://doi.org/1080/07924259.2011.582695.
Soltani N, Soltani-Mazouni N, Quennedey B, Dela chambre J. 1996. Protein synthesis in developing ovaries of mealworm under in vivo and in vitro conditions: Effects of diflubenzuron. Journal of Stored Products Research 32 (3), 205-212. https://doi.org/10.1016/S0022-474X(96)00020-3
Sridhara S, Lee Vaughan H. 2013. Tebufenozide disrupts ovarian development and function in silkmoths. Insect Biochemistry and Molecular Biology 43(12), 1087-1099. https://doi.org/10.1016/j.ibmb.2013.09.002
Telfer WH. 2009. Egg formation in Lepidoptera. Journal of Insect Science 9 (50), 1-21. https://doi.org/10.1673/031.009.5001
Vallae D. 1993. Vitellogenesis in insects and other groups A review, Member Institute. Oswaldo, Cruz (88), 1-26.
Wyatt G, Pan M. 1978. Insect plasma proteins. Annual Review of Biochemistry (47), 779-817.
Kirane-Amrani Leila, Bakli Djihen, TazirAsma, Soltani Noureddine (2018), Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae); IJB, V12, N4, April, P16-22
https://innspub.net/effect-of-methoxyfenozide-on-synthesis-of-major-proteins-in-ovaries-of-ephestia-kuehniella-zell-lepidoptera-pyralidae/
Copyright © 2018
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0