Effect of methyl jasmonate elicitation on biomass, gene expression and saponin accumulation in Bacopa monnieri
Paper Details
Effect of methyl jasmonate elicitation on biomass, gene expression and saponin accumulation in Bacopa monnieri
Abstract
Bacopa monnieri (known as brahmi) is an important medicinal plant in Thailand containing numerous bacoside-saponin compounds and widely used for pharmacological activities. Biosynthesis is regulated by many enzymes and also affected by cellular and environmental factors. The aim of this study was to enhance the biomass (dry weight), key gene expression, and production of saponin in brahmi through the process of methyl jasmonate (MeJA) elicitation. Results showed that a low concentration (100𝜇M) of MeJA treatment had minimal effect and slightly decreased the biomass (dry weight) of the plant (31.3±1.1mg/ plant), compared to untreated MeJA (38.1±2.7 mg/plant). By contrast, higher concentrations of MeJA treatment (200-800𝜇M) resulted in a highly significant decrease in plant biomass. Gene expression of BmAACT was significantly up-regulated at 1-3 hours but started to down-regulate at 6-168 hours after 100µM MeJA-treated brahmi, eventually reaching the normal level of untreated control. BmOSC expression was significantly up-regulated at 6-12 hours but started to down-regulate at 48 hours after 100µM MeJA-treated brahmi, returning to a normal level of untreated control. Bacoside contents (A3, II, X, and C) were significantly increased by low and high concentrations of MeJA treatments after both 7 and 14 days, compared to untreated control. Bacoside accumulations at 14 days after individual MeJA treatment were significantly higher than at 7 days. Results suggested that low concentrations (100-200µM) of exogenous MeJA application could provide a potential protocol for the enhancement of bacoside contents in brahmi seedlings.
Bansal M, Reddy MS, Kumar A. 2016. Seasonal variations in harvest index and bacoside A contents amongst accessions of Bacopa monnieri (L.) Wettst. Collected from wild populations. Physiology Molecular Biology Plants 22(3), 407-413. https://doi.org/10.1007/s12298-016-0366-y
Chen Q, Yan J, Meng X, Xu F, Zhang W, Liao Y, Qu J. 2017. Molecular Cloning, Characterization, and functional analysis of acetyl-coA C-acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from Ginkgo biloba. Molecules 22(1), 74. https://doi.org/10.3390/molecules22010074
Cui L, Wang ZY, Zhou XH. 2012. Optimization of elicitors and precursors to enhance valtrate production in adventitious roots of Valeriana amurensis Smir. ex Kom. Plant Cell, Tissue and Organ Culture 108, 411-420. https://doi.org/10.1007/s11240-011-0052-2
Deepak M, Sangli GK, Arun PC, Amit A. 2005. Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC. Phytochemical Analysis 16, 24-29. https://doi.org/ 10.1002/pca.805
Dhar N, Rana S, Razdan S, Bhat WW, Hussain A, Dhar RS, Vaishnavi S, Hamid A, Vishwakarma R, Lattoo SK. 2014. Cloning and Functional Characterization of Three Branch Point Oxidosqualene Cyclases from Withania somnifera (L.) The Journal of Biological Chemistry 289(24), 17249-7267. https://doi.org/10.1074/jbc.M114.57191
Faisal M, Alatara AA, El-Sheikha MA, Abdel-Salama EM, Qahtana AA. 2018. Thidiazuron induced in vitro morphogenesis for sustainable supply of genetically true quality plantlets of Brahmi. Industrial Crops & Products 118, 173-179. https://doi.org/10.1016/j.indcrop.2018.03.054
Gubbannavar JS, Harisha CR, Chandola HM. 2012. A comparative study of roots of Bacopa monnieri (L.) Pennel and Bacopa floribunda (R.Br.) Wettst. International Journal of Pharmacognosy and Phytochemical Research 4(1), 8-11.
Hebert LE, Weuve J, Scherr PA, Evans DA. 2013. Alzheimer disease in the United States (2010-2050) estimated using the 2010 census. Neurology 80, 1778-1783. https://doi.org/10.1212/wnl.0b013e3
Jansen R, Brogan B, Whitworth A, Okello E. 2014. Effects of five ayurvedic herbs on locomotor behaviour in a Drosophila melanogaster parkinson’s disease model. Phytotherapy Research 28, 1789-1795. https://doi.org/10.1002/ptr.5199
Jeena GS, Fatima S, Tripathi P, Upadhyay S, Shukla RK. 2017. Comparative transcriptome analysis of shoot and root tissue of Bacopa monnieri identifies potential genes related to triterpenoid saponin biosynthesis. BMC Genomics 18, 490. https://doi.org/10.1186/s12864-017-3865-5
Khojasteh A, Mirjalili MH, Palazon J, Eibl R, Cusido RM. 2016. Methyl-Jasmonate enhanced production of rosamarinic acid in cell cultures of Satureja khuzistanica in a bioreactor. Engineering in life science 16(8), 740-749. https://doi.org/10.1002/ elsc.201600064
Largia MJV, Pothiraj G, Shilpha J, Ramesh M. 2015. Methyl jasmonate and salicylic acid synergism enhances bacoside A content in shoot cultures of Bacopa monnieri (L.) Plant Cell Tissue and Organ Culture 122, 9-20 https://doi.org/10. 1007/s11240-015-0745-z
Liu H, Meng F, Miao H, Chen S, Yin T, Hu S, Shao Z, Liu Y, Gao L, Zhu C, Zhang B, Wang Q. 2018. Effects of postharvest methyl jasmonate treatment on main health-promoting components and volatile organic compounds in cherry tomato fruits. Food Chemistry 263, 194-200. https://doi.org/10.1016/j.foodchem.2018.04.124
Majumdar S, Garai S, Jha S. 2011. Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulated production of bacopasaponins in transformed calli and plants. Plant Cell Reports 30, 941-954. https://doi.org/10.1007/s00299-011-1035-9
Mendoza D, Cuaspud O, Arias JP, Ruiz O, Arias M. 2018. Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana. Biotechnology Reports 19, e00273. https://doi.org/10.1016/j.btre.2018.e00273
Ncube B, Finnie JF, Van Staden J. 2012. Quality from the field: The impact of environmental factors as quality determinants in medicinal plants. South African Journal of Botany 82, 11-20. https://doi.org/10.1016/j.sajb.2012.05.009
Pandareesh MD, Anand T, Bhat PV. 2014. Cytoprotective propensity of Bacopa monnieri against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells. Cytotechnology 68, 157-172. https://doi.org/10.1007/s10616-014-9767-3
Piyabhan P, Wetchateng T. 2014. Neuroprotective effects of Bacopa monnieri (brahmi) on novel object recognition and nmdar1 immunodensity in the prefrontal cortex, striatum and hippocampus of sub-chronic phencyclidine rat model of schizophrenia. Journal of the Medical Association of Thailand 97, S50-S55.
Rauf K, Subhan F, Abbas M, Ali SM, Ali G, Ashfaq M, Abbas G. 2014. Inhibitory effect of bacopasides on spontaneous morphine withdrawal induced depression in mice. Phytotherapy Research 28, 937-939.
Saeed S, Ali H, Khan T, Kayani W, Khan MA. 2017. Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant. Physiology and Molecular Biology of Plants 23(1), 229-237.
Sangeetha N, Ganesh D. 2011. Optimization of protocol for in vitro polyploidization in genetic improvement of Bacopa monnieri L. International Journal of Biological Technology 2(2), 28-34.
Sharma P, Yadav S, Srivastava A, Shrivastava N. 2013. Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopa monnieri. Biotechnology Letters 35, 1121-1125.
Singh A, Dwivedi P. 2018. Methyl-jasmonate and salicylic acid as potent elicitors for secondary metabolite production in medicinal plants: A review. Journal of Pharmacognosy and Phytochemistry 7(1), 750-757.
Singh R, Ramakrishna R, Bhateria M, Bhatta RS. 2014. In vitro evaluation of Bacopa monnieri extract and individual constituents on human recombinant monoamine oxidase enzymes. Phytotherapy Research 28, 1419-1422.
Sukito A, Tachibana S. 2016. Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba. Bioresources and Bioprocessing 3, 24.
Thiem B, Kikowska M, Maliński MP, Kruszka D, Napierała M, Florek E. 2017. Ecdysteroids: production in plant in vitro cultures. Phytochemistry Reviews 16(4), 603-622.
Tripathi N, Chouhan DS, Saini N, Tiwari S. 2012. Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from central India using RAPD and ISSR analysis. 3 Biotech. 2, 327-336.
Vishwakarma RK, Ruby, Singh S, Sonawane PD, Srivastava S, Kumari U, Kumar RJS, Khan BM. 2013a. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene (AACT) of Brahmi (Bacopa monniera). Plant Molecular Biology Reporter 31, 547-557.
Vishwakarma RK, Sonawane PD, Singh S, Kumari U, Ruby, Khan BM. 2013b. Molecular characterization and differential expression studies of an oxidosqualene cyclase (OSC) gene of Brahmi (Bacopa monniera). Physiology and Molecular Biology of Plants 19(4), 547–553.
Yadav A, Ahmad J, Chaudhary AA, Ahmad A. 2012. Development of Sequence Characterized Amplified Region (SCAR) Marker for the Authentication of Bacopa monnieri (L.) Wettst. European Journal of Medicinal Plants 2(3), 186-198.
Yin J, Li C, Zhan Y, Sun H, Gong Y, Zhang M, Xiao J. 2016. The response of physiological characteristics, expression of OSC genes, and accumulation of triterpenoids in Betula platyphylla Sukto MeJA and SA treatment. Plant Molecular Biology Reporter 34(2), 427-39.
Yin J, Li CX, Sun HR, Wang ZH, Xiao JL, Zhan YG, Zhang MY. 2014. The Physiological Characteristics, Expression of Oxidosqualene cyclase genes and accumulation of triterpenoids in white birch (Betula platyphylla suk) Saplings by SA and MeJA treatment. Journal of Plant Biochemistry & Physiology 2(2), 1000129.
Zhan C, Li X, Zhao Z, Yang T, Wang X, Luo B, Zhang Q, Hu Y, Hu X. 2016. Comprehensive analysis of the triterpenoid saponins Biosynthetic Pathway in Anemone flaccida by Transcriptome and Proteome Profiling. Frontiers in Plant Science 7, 1094.
Zhao JL, Zhou LG, Wu JY. 2010. Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Applied Microbiology and Biotechnology 87, 137-144.
Waranya Bunjan, Kawee Sujipuli, Surisak Prasarnpun (2018), Effect of methyl jasmonate elicitation on biomass, gene expression and saponin accumulation in Bacopa monnieri; IJB, V13, N4, October, P369-377
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